Diethyl 1,2-cyclopentanedicarboxylate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994-12-07 to 1994-12-09

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Version / remarks:

31st July 1992

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Diethyl 1,2-cyclopentanedicarboxylate

EC Number:

291-751-5

EC Name:

Diethyl 1,2-cyclopentanedicarboxylate

Cas Number:

90474-13-8

Molecular formula:

C11H18O4

IUPAC Name:

1,2-diethyl cyclopentane-1,2-dicarboxylate

Test material form:

other: liquid

Details on test material:

- Name of test material (as cited in study report): 852 DIESTER
- Physical state: liquid
- Analytical purity: 96.8 %
- Purity test date: 1994-11-18
- Lot/batch No.: 878601/007 007/95
- Expiration date of the lot/batch: 1996-10-13
- Storage condition of test material: cool (ambient temperature) and away from light

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: the test solution was dissolved directly in water producing a solution of 100 mg/l. As the test substance is known to be stable in water, the solution was agitated for around 24 hours in an attempt to solubilise the test substance completely.
- Sampling method and sample storage conditions before analysis: a sample of 5 ml was taken from all test solution containers at the beginning of the test and frozen. A further sample totaling 4 ml was taken from the test vessels containing daphnids at each concentration at the end of the test and frozen until analysis.

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia Magna
- Strain: STRAUS-clone 5 (A)
- Source: Centre Technique du Bois et de l'Ameublement, 75012 Paris (France), breeding at CIT
- Age at study initiation (mean and range, SD): between 6 and 24 hours
- Feeding during test: no

ACCLIMATION
- Acclimation period: none. Animals were held in water of the same quality as the test water from birth, until use in the test

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Test conditions

Hardness:

between 294 and 336 mg/l as CaCO3

Test temperature:

between 17.9 and 19°C

pH:

between 7.47 and 8.21

Dissolved oxygen:

between 7.8 and 8.3 mg/l (>60 %) (after correction for barometric pressure)

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

> 10 - < 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

The test substance 852 DIESTER tested as a solution on Daphnia magna in a 48-hour static test resulted in immobilisation at a nominal concentration of 100 mg/l while the nominal concentrations of 1 and 10 mg/l (the latter measured at 6.04 mg/l) resulted in no immobilisation.
It is recommended that the concentrations should be based on nominal values and that the test substance should not be classified.