Diethyl 1,2-cyclopentanedicarboxylate

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1994-11-19 to 1994-12-02

Reliability:

1 (reliable without restriction)

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1995

Report date:

1995

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Version / remarks:

and Commission Directive 92/69/EEC (C1, 31st July 1992)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Diethyl 1,2-cyclopentanedicarboxylate

EC Number:

291-751-5

EC Name:

Diethyl 1,2-cyclopentanedicarboxylate

Cas Number:

90474-13-8

Molecular formula:

C11H18O4

IUPAC Name:

1,2-diethyl cyclopentane-1,2-dicarboxylate

Test material form:

other: liquid

Details on test material:

- Name of test material (as cited in study report): 852 DIESTER
- Physical state: liquid
- Analytical purity: 96.8 %
- Purity test date: 1994-11-18
- Lot/batch No.: 878601/007 007/95
- Expiration date of the lot/batch: 1996-10-13
- Storage condition of test material: at room temperature and protected from light

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

- Concentrations: the stock solution was prepared by dissolving 100 mg of the test substance directly per litre of LC reconstituted water. Test solutions were prepared by further dilution of the stock solution with water to provide a geometric series of concontrations: 0, 1, 10 and 100 mg/l
- Sampling method and Sample storage conditions before analysis:
At T0 hours, 4 ml samples were taken from each test solution container and frozen at -20°C.
At T72 hours, 1.5 ml samples were taken from all 3 test replicates at each concentration, pooled and frozen. At the same time, 1 ml was taken from each of the controls and the samples pooled and frozen.

Test organisms

Test organisms (species):

Desmodesmus subspicatus (previous name: Scenedesmus subspicatus)

Details on test organisms:

TEST ORGANISM
- Strain: CCAP 276/20
- Source (laboratory, culture collection): Culture Centre of Algae and Protozoa, Institute of Freshwater Ecology, Far Sawrey, Ambleside, Cumbria, LA 22 OLP, UK
- Age of inoculum (at test initiation):
- Method of cultivation: cultured at C.I.T. ; algae is cultivated for 7 days before harvesting for use in a new culture. pH is verified at 7.5 +/- 0.3 ; conductivity is 260 +/- 20 microScm-1 ; temperature is between 21 and 25°C ; Illumination 24 hours per day at approximatively 2000 lux

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

72 h

Test conditions

Test temperature:

22.9-23.5°C

pH:

7.23-8.20

Nominal and measured concentrations:

nominal: 1 x 10e4 cells/ml

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 10 - < 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth rate

Effect concentrations 2

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 10 - < 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

biomass

Effect concentrations 3

Duration:

72 h

Dose descriptor:

NOEC

Effect conc.:

ca. 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Remarks:

test substance concentrations remain > 78 % of the nominal concentration throughout the test

Conclusions:

Under our laboratory conditions, the test substance, 852 DIESTER, was toxic to Scenedesmus subspicatus in a static test at 100 mg/l but non-toxic at 1 and 10 mg/l.
The NOEC is calculated to be 10 mg/l

852 DIESTER should not be classified.