Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
from 04 to 13 May 1994
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
Although the test was performed according to an internationally recognized guideline (but not under GLP compliance), no information was available about the purity of the test substance. There was no analytical monitoring performed during the study. However, the available water solubility data indicates that 2-fluoronitrobenzene is soluble (water solubility = 1607 mg/L, see IUCLID section 4.8) and thus, there should have been no solubility issue at the concentrations tested during this ecotoxicity study (maximum 100 mg/L).

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1994
Report Date:
1994

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Version / remarks:
1984
Deviations:
not specified
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
liquid
Details on test material:
Identity: 2-FLUORONITROBENZENE
CAS number: 1493-27-2

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: Test solutions were prepared by direct dispersion into test medium.
- Controls: Yes (test medium without test item).
No further data.

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Hardness:
270 mg/L CaCO3
Test temperature:
21°C
pH:
7.8 +/- 0.2 adjusted if necessary with NaOH or HCl (no information on whether such adjustment was actually performed)
Nominal and measured concentrations:
Nominal: 1.0, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessels: 200 mL
- Aeration: no
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Reconstituted water was prepared as follows:
a) CaCl2.2H2O 11.76 g/L
b) MgS04.7H2O 4.93 g/L
c) NaHC03 2.59 g/L
d) KCl 0.23 g/L
25 mL of each of solutions a) to d) were added to deionised water (conductivity < 5 µS/cm) to reach a final volume of 1 L.

EFFECT PARAMETERS MEASURED
Immobilization after 24 and 48 hours

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.8
- Range finding study: Yes
Test concentrations: 0, 0.1, 1, 10 and 100 mg/L
Results used to determine the conditions for the definitive study: 100 % mortality at 100 mg/L and 70% at 10 mg/L.
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
9.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
Other effect concentrations:
24h-EC50 = 26 mg/L.
24h-NOEC = 18 mg/L
48h-NOEC = 5.6 mg/L.

Reported statistics and error estimates:
Analysis of the immobility data was performed by the moving average method of Thompson (1947).

Any other information on results incl. tables

Cumulative immobilisation data for Daphnia magna exposed for 48 hours to 2-fluoronitrobenzene

Nominal Concentration

(mg/L)

Cumulative immobilized Daphnia

(initial population: 10 per replicate)

24 hours

48 hours

R1

R2

Total

%

R1

R2

Total

%

Control

0

0

0

0

0

0

0

0

1

0

0

0

0

0

0

0

0

1.8

0

0

0

0

0

0

0

0

3.2

0

0

0

0

0

0

0

0

5.6

0

0

0

0

0

0

0

0

10

0

0

0

0

6

8

14

70

18

0

0

0

0

9

8

17

85

32

8

9

17

85

10

10

20

100

56

10

10

20

100

10

10

20

100

100

10

10

20

100

10

10

20

100

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Remarks:
The criterion according to which there should be no immobility in the controls is fulfilled, but there is no information reported regarding the dissolved oxygen concentrations at the end of the test (which should be > 3 mg/L).
Conclusions:
A 48h-EC50 (Daphnia magna) of 9.7 mg/L was determined; indicating that 2-fluoronitrobenzene is toxic to aquatic invertebrates.
Executive summary:

The acute toxicity of 2-fluoronitrobenzene to Daphnia magna was evaluated according to the OECD 202 guideline, but not under GLP conditions. Groups of 10 daphnids were exposed for 48 hours under static conditions to a control treatment and to 2-fluoronitrobenzene concentrations of 1, 1.8, 3.2, 5.6, 10, 18, 32, 56 and 100 mg/L in duplicates. Mobility was observed after 24 and 48 hours. There was no analytical monitoring of the test item concentrations during the exposure. The 48h-EC50 was set equal to 9.7 mg/L; indicating that 2-fluoronitrobenzene was toxic to aquatic invertebrates under the tested experimental conditions.