N-(4-{[3-(dimethylamino)propyl]sulfamoyl}phenyl)-2-[(2-methoxy-4-nitrophenyl)diazenyl]-3-oxobutanamide

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

16.-18.03.2009

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP - Guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Test guideline 3

Qualifier:

according to guideline

Guideline:

ISO 6341 (Water quality - Determination of the Inhibition of the Mobility of Daphnia magna Straus (Cladocera, Crustacea))

Test guideline 4

Qualifier:

according to guideline

Guideline:

other: Guidance on aquatic toxicity testing of difficult substances and mixtures, OECD series on testing and assessment number 23 (2000)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

N-(4-{[3-(dimethylamino)propyl]sulfamoyl}phenyl)-2-[(2-methoxy-4-nitrophenyl)diazenyl]-3-oxobutanamide

EC Number:

700-222-4

Cas Number:

1065519-44-9

Molecular formula:

C22H28N6O7S

IUPAC Name:

N-(4-{[3-(dimethylamino)propyl]sulfamoyl}phenyl)-2-[(2-methoxy-4-nitrophenyl)diazenyl]-3-oxobutanamide

Details on test material:

- Name of test material (as cited in study report): NO.408 YELLOW
- Physical state: powder
- Analytical purity: 98.6%
- Lot/batch No.: 081105-1
- Expiration date of the lot/batch: 04. November 2009
- Storage condition of test material: at room temperature in the dark
- Other: solubility in water: 0.38 mg/L

Sampling and analysis

Analytical monitoring:

yes

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Preparation of test solutions started with a stock solution prepared at a loading rate of 100 mg/L. A 15-minute treatment period with ultrasonic waves was followed by a magnetic stirring period of 45 h to accelerate the dissolving of the test substance in the test medium. This resulted in a solution that still ocntained some undissolved material, which was consequently removed by filtration through a 0.45 μm membrane filter (Whatman, RC55).
- Controls: Test medium without test substance or other additives.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Strain: Straus, 1820
- Source: In-house laboratory culture with a known history
- Age at study initiation (mean and range, SD): < 24 h
- Feeding during test: no

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

180 mg CaCO3/L

Test temperature:

20.3 °C - 21.2 °C

pH:

see table below

Dissolved oxygen:

see table below

Nominal and measured concentrations:

Nominal concentrations: 1.0, 10 and 100% of a 0.45 μm filtered solution prepared at a loading rate of 100 mg/L
Measured concentrations: Analysis of the samples taken from the filtrate at the start of the combined limit/range-finding test showed a measure concentration of 0.256 mg/L. During the exposure period the measured concentration decreased to 0.113 mg/L. The average exposure concentration being the geometric mean was calculated to correspond to 0.17 mg/L. This concentration exceeded the maximum solubility that was limited to 19.8 μg/L.

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 100 mL all-glass test vessels containing 80 mL of test solution
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4 (highest concentration) and 2 (remaining concentrations)
- No. of vessels per control (replicates): 4


OTHER TEST CONDITIONS
- Photoperiod: 16 h light, 8 h dark


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility was monitored daily


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 10
- Justification for using less concentrations than requested by guideline: study is a combined limit/range-finding test

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 0.17 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

- Mortality of control: 0%
- Effect concentrations exceeding solubility of substance in test medium: The average exposure concentration of 0.17 mg/L exceeded the maximum solubility that was limited to 19.8 μg/L.

Results with reference substance (positive control):

- EC50: 0.30 mg/L with a 95% confidence interval between 0.25 and 0.35 mg/L

Any other information on results incl. tables

Table 2: Acute immobilisation of daphnids after 24 and 48 hours in the combined limit/range-finding test

Test group NO.408 YELLOW (% of filtrate)	Vessel number	Number of Daphnia exposed	Response at 24 h		Response at 48 h
			Number	Total %	Number	Total %
Control	A B C D	5 5 5 5	0 0 0 0	0	0 0 0 0	0
1.0	A B	5 5	0 0	0	0 0	0
10	A B	5 5	0 0	0	0 0	0
100 (0.17 mg/L^1)	A B C D	5 5 5 5	0 0 0 0	0	1 1 1 1	20

¹Average exposure concentration