Strontium bis[2-chloro-5-[(2-hydroxy-1-naphthyl)azo]toluene-4-sulphonate]

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

disregarded due to major methodological deficiencies

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: Guideline study, however DMSO was used as solubliser

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1994

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: OECD guideline 202

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Calcium 3-hydroxy-4-[(4-methyl-2-sulphonatophenyl)azo]-2-naphthoate

EC Number:

226-109-5

EC Name:

Calcium 3-hydroxy-4-[(4-methyl-2-sulphonatophenyl)azo]-2-naphthoate

Cas Number:

5281-04-9

IUPAC Name:

calcium 3-hydroxy-4-[(4-methyl-2-sulfonatophenyl)diazenyl]-2-naphthoate

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): DMSO

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Feeding during test: daily
- Food type: green algae

Study design

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Test conditions

Test temperature:

20 - 22 ± °1C

Nominal and measured concentrations:

Nominal test concentrations: 3-300 mg/l, control of DMSO:HCO-40= 9:1 (100 mg/kg) and laboratory water control.

Details on test conditions:

TEST SYSTEM
- Test vessel: beaker (500 ml)
- Fill volume: 400 ml
- Renewal rate of test solution (frequency/flow rate): renewal of test substance every 48 hours.
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
- Biomass loading rate:

OTHER TEST CONDITIONS
- Photoperiod: 8:16 h day night regime

VEHICLE CONTROL PERFORMED: yes

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

14 d

Dose descriptor:

EC50

Effect conc.:

4.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: The test is classified as disregarded study since DMSO was used as solubiliser. DMSO is known to increase the toxicity of substances.

Effect concentrations 2

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

9.1 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: The test is classified as disregarded study since DMSO was used as solubiliser. DMSO is known to increase the toxicity of substances.

Effect concentrations 3

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

3 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: mobility and reproduction

Remarks on result:

other: The test is classified as disregarded study since DMSO was used as solubiliser. DMSO is known to increase the toxicity of substances.

Effect concentrations 4

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

9.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: mortality and reproduction

Remarks on result:

other: The test is classified as disregarded study since DMSO was used as solubiliser. DMSO is known to increase the toxicity of substances.