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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Description of key information

The 48h EC50 (immobilisation) value for Coagulant 122 (solid) with Daphnia magna is >210 mg/l.
The study was conducted in accordance with EEC Methods for Determination of Ecotoxicity Annex to Directive 92/69/EEC (O.J. No. L383A, 29.12.92) Part C, Method 2 "Acute toxicity to Daphnia" and the OECD Guideline for Testing of Chemicals No. 202, Part I "Daphnia Acute Immobilisation Test".

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
210 mg/L

Additional information

Cumulative immobilisation results expressed in terms of mean measured concentration. Measured concentrations ranged from 90-108 % of nominal at 0 hours and 30-90 % of nominal at 48 hours.

 Nominal concentration (mg/l)  Number of samples analysed  Mean measured concentration (mg/l)  % Nominal
 Control 2 - -
 10 2  3.8  38*
 22 2  5.7  26*
 46  2  25  54
 100  2  82  82
 220  211  96

* 48 hr values for mean measured calculations assumed to 1.6 mg/l (limit of detection).

Analysis of the immobility data gave the following results:

 Time (h)  EC50 (mg/l)  95% confidence limits (mg/l)
 24  >210 -
 48  >210  -

Highest test concentration resulting in 0% immobilisation: ≥210 mg/1

Lowest test concentration resulting in 100% immobilisation: >210 mg/1

Individual pH, temperature and dissolved oxygen values remained within acceptable limits throughout

the duration of the study (table represent mean values):

 Nominal concentration (mg/l)     Measured concentration (mg/l)     Mean values      
 pH  mgO2/l  T°C
Control Control 7.6 8.6 21
 10  3.8  7.6  8.5  21
 22  5.7  7.3  8.3  21
 46  25  7.5  7.8  21
 100  82  7.5  7.8  21
 220  210  7.8  7.7  21