Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 471-980-9 | CAS number: 1016986-95-0
Hydrolysis
Administrative data
- Endpoint:
- hydrolysis
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- Testing phase 2005-09-01 to 2005-10-13. Final report completed and signed 2005-11-30
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Meets the criteria for classification as Reliable without restriction according to Klimisch et al (1997)
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 005
- Report date:
- 2005
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: 92/69/EEC, C7
- Deviations:
- no
- GLP compliance:
- yes
Test material
- Reference substance name:
- -
- EC Number:
- 471-980-9
- EC Name:
- -
- Cas Number:
- 1016986-95-0
- Molecular formula:
- Hill formula: C42 H24 Lim N9 Nan O15 S6 m+n=5 3=
- IUPAC Name:
- 7-{2-[2-(2-{5-cyano-4-methyl-2,6-bis[(4-sulfophenyl)amino]pyridin-3-yl}diazen-1-yl)-4-(naphthalen-2-yl)-1,3-thiazol-5-yl]diazen-1-yl}naphthalene-1,3,5-trisulfonic acid trilithium hydride disodium hydride
Constituent 1
- Radiolabelling:
- no
Study design
- Analytical monitoring:
- yes
- Details on sampling:
- Aliquots of the sample solutions were taken from the flasks at various times and the pH of each solution recorded. The concentration of the sample solution was determined by HPLC
- Buffers:
- - pH: 4
- Composition of buffer: 0.01 mol dm3 Potassium hydrogen phthalate
- pH: 7
- Composition of buffer: 0.006 mol dm3 Disodium hydrogen orthophosphate (anhydrous), 0.004 mol dm3 Potassium dihydrogen orthophosphate, 0.004 mol dm3 Sodium chloride
- pH: 9
- Composition of buffer: 0.002 mol dm3 Disodium tetraborate, 0.004 mol dm3 Sodium chloride.
All buffer solutions were filtered through a 0.2 um membrane filter to ensure they were sterile. The solutions were subjected to ultrasonication and degassing with nitrogen to minimise dissolved oxygen content. - Details on test conditions:
- Sample solutions were prepared in stoppered glass flasks at a nominal concentration of 1 g/l in the three buffer solutions. The solutions were shielded from light whilst maintained at the test temperature. Sample solutions at pH 4, 7 and 9 were maintained at 50.0 +/- 0.5 C for a period of 5 days. Aliquots of the sample solutions were taken from the flasks at various times and the pH of each solution recorded. The concentration of the sample solution was determined by HPLC.
Duration of testopen allclose all
- Duration:
- 5 d
- pH:
- 4
- Temp.:
- 50 °C
- Initial conc. measured:
- 0.846 g/L
- Duration:
- 5 d
- pH:
- 7
- Temp.:
- 50 °C
- Initial conc. measured:
- 0.848 g/L
- Duration:
- 5 d
- pH:
- 9
- Temp.:
- 50 °C
- Initial conc. measured:
- 0.861 g/L
- Number of replicates:
- 2
- Positive controls:
- no
- Negative controls:
- no
Results and discussion
- Transformation products:
- not measured
Total recovery of test substance (in %)open allclose all
- % Recovery:
- 101
- pH:
- 4
- Temp.:
- 50 °C
- Duration:
- 2.4 h
- % Recovery:
- 98.1
- pH:
- 4
- Temp.:
- 50 °C
- Duration:
- 120 h
- % Recovery:
- 100
- pH:
- 7
- Temp.:
- 50 °C
- Duration:
- 2.4 h
- % Recovery:
- 98.6
- pH:
- 7
- Temp.:
- 50 °C
- Duration:
- 120 h
- % Recovery:
- 101
- pH:
- 9
- Temp.:
- 50 °C
- Duration:
- 2.4 h
- % Recovery:
- 98.3
- pH:
- 9
- Temp.:
- 50 °C
- Duration:
- 120 h
Dissipation DT50 of parent compoundopen allclose all
- pH:
- 4
- Temp.:
- 25 °C
- DT50:
- > 1 yr
- pH:
- 7
- Temp.:
- 25 °C
- DT50:
- > 1 yr
- pH:
- 9
- Temp.:
- 25 °C
- DT50:
- > 1 yr
Any other information on results incl. tables
At each of pH = 4, 7 and 9, less than 10% hydrolysis occurred after 5 days at 50°C, equivalent to a half-life greater than 1 year at 25°C
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- Less than 10% hydrolysis occurred after 5 days at 50 C at pH 4, 7 and 9. Therefore, the estimated half life at 25 C was >1 year at pH 4, 7 and 9.
- Executive summary:
Introduction
This study was undertaken to assess hydrolysis as a function of pH. This was carried out using Method C7 of Commission Directive 92/69/EEC (which constitutes Annex V of Council Directive 67/548/EEC).
Results & Conclusions
At pH 4, 7 and 9 less than 10% hydrolysis was detected after 5 days at 50 C. This is equivalent to a half life greater than 1 year at 25 C.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
