4-tert-butylbenzaldehyde

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Basic data given: comparable to standards

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1981

Report date:

1981

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Principles of method if other than guideline:

The study was conducted according to an internal BASF method.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

4-tert-butylbenzaldehyde

EC Number:

213-367-9

EC Name:

4-tert-butylbenzaldehyde

Cas Number:

939-97-9

Molecular formula:

C11H14O

IUPAC Name:

4-tert-butylbenzaldehyde

Details on test material:

- Name of test material (as cited in study report): 4-tert-butylbenzaldehyde (p-tert.butyl-benzaldehyde)
- Analytical purity: 98 %

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Fasting period before study: 15-20 h prior to application of the test material
- Diet: Herilan MRH Haltung (H Eggersmann KG), ad libitum
- Water: ad libitum

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

CMC (carboxymethyl cellulose)

Details on oral exposure:

Application volume: 10 ml/kg bw
Concentration of the test material in vehicle: 0.147, 0.215, 0.316, 0.464 and 0.681 % (w/v)

Doses:

14.7, 21.5, 31.6, 46.4 and 68.1 mg/kg bw

No. of animals per sex per dose:

5 animals per sex per dose

Control animals:

no

Details on study design:

Observation period: 14 d.
Body weight determined before the start of the study, after 2-4 days, 7 days and 9-13 days.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs

Statistics:

Statistics: following Finney D.J.: Probit analysis, Cambridge University Press, 3rd ed., 1971

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

50.6 mg/kg bw

Mortality:

For details on mortality, see "Remarks on results ...".

Clinical signs:

other: Symptoms observed - Dyspnoe - Apathy - Abnormal position - Staggering - Atony - Narcotic (high dose only) - No pain reflex (high dose only) - No corneal reflex (high dose only) - Spastic movement - Ruffled fur - Diarrhea - Cyanosis - Exsiccosis - Exophta

Gross pathology:

Dead animals:
- Heart: acute dilatation on the right side; acute congestion hyperemia.
- Liver: clay-like coloured due to slightly broadened Lobuli hepatici.
- Kidneys: brightened (nephrosis).

Sacrificed animals:
No substance-related effects.

Any other information on results incl. tables

Table 1: Mortality data
 
Dose        Mortality after
mg/kg       1 h    1 d    2 d   7 d   14 d
-----------------------------------------------
68.1  M     0/5    2/5    2/5   3/5   3/5  
      F     0/5    5/5    5/5   5/5   5/5
46.4  M     0/5    2/5    2/5   2/5   2/5
      F     0/5    2/5    2/5   2/5   2/5
31.6  M     0/5    0/5    0/5   0/5   0/5
      F     0/5    1/5    1/5   1/5   1/5    
21.5  M     0/5    0/5    0/5   0/5   0/5
      F     0/5    0/5    0/5   0/5   0/5
14.7  M     0/5    0/5    1/5   1/5   1/5
      F     0/5    0/5    0/5   0/5   0/5
------------------------------------------------
M = males, F = females