Bis(4-methylbenzoyl)peroxide

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was performed under GLP according to protocol. It was reviewed in the HPV program and recorded in the OECD SIDS dossier as reliability 1.

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Oryzias latipes

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Nominal and measured concentrations:

Nominal: 35.0, 45.5, 59.2, 76.9 and 100 mg/L

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

64 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Validity criteria fulfilled:

yes

Conclusions:

This study is reliable. It is performed according to OECD guideline 203 under GLP and it was reviewed during the HPV process. The calculated endpoints are adequate for C&L and risk assessment purposes.

Executive summary:

An acute toxicity test of p-toluic acid was conducted for Oryzias latipes (MOE Japan, 2003). The exposure was undertaken under semi-static condition with every 24 hours renewal at the concentrations of 35.0, 45.5, 59.2, 76.9 and 100 mg/L in nominal with a blank control. No vehicle was used. Ten fish per concentration were used. The measured concentrations in each treatment at initial (just after renewal) and after a 24 hrs were 98.9 – 100.4 % and 99.3 – 102.4 % of the nominal, respectively. At the control and treatment groups up to 45.1 mg/L (in the mean measured concentration) no mortality were observed during a 96 h of test period. The mortalities at 58.9, 76.5 and 101.4 mg/L (mean measured concentrations) were 10, 100, and 100 %, respectively. Based on measured concentration the 96 h LC₅₀(95% confidence limits) of 64 (57 – 74) mg/L was calculated by a probit method. In the test the pH of test water of 76.5 and 101.4 mg/L were 5.3 and 4.7, respectively. Thus the item was stable under the experimental condition. At these treatment groups all fish were died within 24 hours.The test is regarded as high reliability because no deviations from the OECD Test Guideline 203 occurred, although adjustment of pH was not undertaken.

Description of key information

An acute toxicity test of p-toluic acid was conducted for Oryzias latipes (MOE Japan, 2003). The exposure was undertaken under semi-static condition with every 24 hours renewal at the concentrations of 35.0, 45.5, 59.2, 76.9 and 100 mg/L in nominal with a blank control. No vehicle was used. Ten fish per concentration were used. The measured concentrations in each treatment at initial (just after renewal) and after a 24 hrs were 98.9 – 100.4 % and 99.3 – 102.4 % of the nominal, respectively. At the control and treatment groups up to 45.1 mg/L (in the mean measured concentration) no mortality were observed during a 96 h of test period. The mortalities at 58.9, 76.5 and 101.4 mg/L (mean measured concentrations) were 10, 100, and 100 %, respectively. Based on measured concentration the 96 h LC₅₀(95% confidence limits) of 64 (57 – 74) mg/L was calculated by a probit method. In the test the pH of test water of 76.5 and 101.4 mg/L were 5.3 and 4.7, respectively. Thus the item was stable under the experimental condition. At these treatment groups all fish were died within 24 hours.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

64 mg/L

Additional information

Due to rapid hydrolysis of 895-85-2 (see van Dam, 2014), the aquatic results of p-toluic acid are considered most relevant.