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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
skin sensitisation
Remarks:
in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
other: Body responsible for the test
Title:
Unnamed
Year:
1991
Report date:
1991

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: 84/449/EWG, B.6 (Meerschweinchen-Maximierungstest (GPMT):
GLP compliance:
yes
Type of study:
other: 84/449/EWG, B.6 (Meerschweinchen-Maximierungstest (GPMT)

Test material

Constituent 1
Chemical structure
Reference substance name:
2,3,4,5-tetrachlorobenzoylchloride
EC Number:
406-760-3
EC Name:
2,3,4,5-tetrachlorobenzoylchloride
Cas Number:
42221-52-3
Molecular formula:
C7HCl5O
IUPAC Name:
2,3,4,5-tetrachlorobenzoyl chloride
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder

In vivo test system

Test animals

Species:
other: gpg, Ibm:GOHI (SPF)

Study design: in vivo (non-LLNA)

Induction
Concentration / amount:
Concentration of test material and vehicle used at induction:
Intradermal: 0,1 ml
a) Testsubstanz verdünnt mit olivenöl auf 1 % (G/G)
b) Freund's complete Adjuvans 50:50 mit physiologischer
Kochsalzlösung.
c) Testsubstanz verdünnt auf 1 % durch Emulgieren in einem
50:50 Gemisch von Freund's Adjuvans und Olivenöl.
Epidermal: 0,5 ml / 15 % Testsubstanz
Concentration of test material and vehicle used for each challenge:
Epidermal:
Die Filterpapierstücke wurde gesättigt mit:
a) 2,5 % Testsubstanzlösung in Olivenöl
b) Vehikel
Challenge
Concentration / amount:
Concentration of test material and vehicle used at induction:
Intradermal: 0,1 ml
a) Testsubstanz verdünnt mit olivenöl auf 1 % (G/G)
b) Freund's complete Adjuvans 50:50 mit physiologischer
Kochsalzlösung.
c) Testsubstanz verdünnt auf 1 % durch Emulgieren in einem
50:50 Gemisch von Freund's Adjuvans und Olivenöl.
Epidermal: 0,5 ml / 15 % Testsubstanz
Concentration of test material and vehicle used for each challenge:
Epidermal:
Die Filterpapierstücke wurde gesättigt mit:
a) 2,5 % Testsubstanzlösung in Olivenöl
b) Vehikel
No. of animals per dose:
Number of animals in test group: 20
Number of animals in negative control group: 10

Results and discussion

In vivo (non-LLNA)

Resultsopen allclose all
Reading:
1st reading
Hours after challenge:
24
Group:
test chemical
Dose level:
2.5%
No. with + reactions:
20
Total no. in group:
20
Remarks on result:
other: Reading: 1st reading. . Hours after challenge: 24.0. Group: test group. Dose level: 2.5%. No with. + reactions: 20.0. Total no. in groups: 20.0.
Reading:
1st reading
Hours after challenge:
24
Group:
test chemical
Dose level:
0%
No. with + reactions:
0
Total no. in group:
20
Remarks on result:
other: Reading: 1st reading. . Hours after challenge: 24.0. Group: test group. Dose level: 0%. No with. + reactions: 0.0. Total no. in groups: 20.0.
Reading:
2nd reading
Hours after challenge:
48
Group:
test chemical
Dose level:
2.5%
No. with + reactions:
20
Total no. in group:
20
Remarks on result:
other: Reading: 2nd reading. . Hours after challenge: 48.0. Group: test group. Dose level: 2.5%. No with. + reactions: 20.0. Total no. in groups: 20.0.
Reading:
2nd reading
Hours after challenge:
48
Group:
test chemical
Dose level:
0%
No. with + reactions:
0
Total no. in group:
20
Remarks on result:
other: Reading: 2nd reading. . Hours after challenge: 48.0. Group: test group. Dose level: 0%. No with. + reactions: 0.0. Total no. in groups: 20.0.
Reading:
1st reading
Hours after challenge:
24
Group:
negative control
Dose level:
2.5%
No. with + reactions:
1
Total no. in group:
10
Remarks on result:
other: Reading: 1st reading. . Hours after challenge: 24.0. Group: negative control. Dose level: 2.5%. No with. + reactions: 1.0. Total no. in groups: 10.0.
Reading:
1st reading
Hours after challenge:
24
Group:
negative control
Dose level:
0%
No. with + reactions:
0
Total no. in group:
10
Remarks on result:
other: Reading: 1st reading. . Hours after challenge: 24.0. Group: negative control. Dose level: 0%. No with. + reactions: 0.0. Total no. in groups: 10.0.
Reading:
2nd reading
Hours after challenge:
48
Group:
negative control
Dose level:
2.5%
No. with + reactions:
1
Total no. in group:
10
Remarks on result:
other: Reading: 2nd reading. . Hours after challenge: 48.0. Group: negative control. Dose level: 2.5%. No with. + reactions: 1.0. Total no. in groups: 10.0.
Reading:
2nd reading
Hours after challenge:
48
Group:
negative control
Dose level:
0%
No. with + reactions:
0
Total no. in group:
10
Remarks on result:
other: Reading: 2nd reading. . Hours after challenge: 48.0. Group: negative control. Dose level: 0%. No with. + reactions: 0.0. Total no. in groups: 10.0.

Any other information on results incl. tables

Maximum concentration not causing irritating effects in preliminary test: 2.5 %

Signs of irritation during induction: 8 von 10 Tieren der Kontrollgruppe zeigten nach der Applikation eine leichte Hautrötung.

Alle Tiere der Testgruppe zeigten nach der Applikation eine leichte Hautrötung.

"ENGLISH"

8 out of 10 animals from the control group exhibited slight skin skin redness after application.

All of the animals from the test group exhibited skin redness after application.

Evidence of sensitisation of each challenge concentration: 2,5 %: 20/20 Tiere

Other observations:

Es wurden keine systemischen Symptome beobachtet.

Keine Todesfälle.

"ENGLISH"

None

Applicant's summary and conclusion

Interpretation of results:
other: irritant