Registration Dossier
Registration Dossier
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EC number: 929-513-6 | CAS number: 1400584-86-2
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)
- Version / remarks:
- 1992
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-D (Determination of the "Ready" Biodegradability - Manometric Respirometry Test)
- Version / remarks:
- 1992
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- sodium (5s,8s)-8-methoxy-2,4-dioxo-1,3-diazaspiro[4.5]decan-3-ide
- EC Number:
- 929-513-6
- Cas Number:
- 1400584-86-2
- Molecular formula:
- C9H13N2O3 · Na
- IUPAC Name:
- sodium (5s,8s)-8-methoxy-2,4-dioxo-1,3-diazaspiro[4.5]decan-3-ide
- Test material form:
- solid: bulk
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- sewage, predominantly domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): predominantly domestic sewage (Wupper area water authority, STP Odenthal)
- Concentration of sludge: 30 mg/L ss
- Storage conditions: the inoculum was stored at room temperature under continuous shaking with aeration until use for max. 3 days. - Duration of test (contact time):
- 28 d
Initial test substance concentration
- Initial conc.:
- 100 mg/L
- Details on study design:
- TEST CONDITIONS
- Test temperature: 22 °C
- pH: 7.4 - 8.4
- pH adjusted: no
Pre-treatment of the test item
25 mg of the test item were weighed out on aluminium foil. Test item and aluminium foil were added to the test flasks, which had already been filled with 200 mL of mineral medium. Afterwards the flask content was made up to 250 mL with mineral medium containing inoculum to give a test concentration of 100 mg test item/L.
Exposure conditions
Test volume: 250 mL
Test apparatus: Oxi Top System (WTW)
Mixing: 1 magnetic stirrer per test vessel
Incubation time: 28 days
Incubation temperature: 22 ± 1 °C
Pre-treatment of the inoculum:
- the sludge was washed twice by adding mineral medium, centrifuging (10 min at 2000 rpm and 20 °C) and decanting off the supernatant
- 1.066 g of the wet sludge was dried in order to calculate the amount of wet sludge to achieve a concentration of activated sludge of 3 g/L (dry wet) suspended solids
- the calculated amount of sludge was first dissolved in mineral medium and then filled up to a defined end volume
- the inoculum was stored at room temperature under continuous shaking with aeration until use for max. 3 days.
Reference substance
- Reference substance:
- benzoic acid, sodium salt
Results and discussion
% Degradation
- Key result
- Parameter:
- % degradation (O2 consumption)
- Value:
- 0
- Sampling time:
- 28 d
- Details on results:
- The reference compound sodium benzoate showed 84.5 % degradation after 14 days.
BOD5 / COD results
- Results with reference substance:
- 84.5 % degration after 14 days
Any other information on results incl. tables
The test item showed:
0.0 % degradation after 7 days
0.0 % degradation after 14 days
0.0 % degradation after 21 days
0.0 % degradation after 28 days
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- under test conditions no biodegradation observed
- Conclusions:
- The substance is considered to be "Not Ready Biodegradable".
- Executive summary:
The study was conducted in accordance with the Council Directive 92/69/EEC Method C.4-D "Manometric Respirometry Test" (1992). This test method is in all essential parts identical with OECD Guideline 301 F.
A solution of the item in a mineral medium was inoculated and incubated for 28 d under aerobic conditions. During this period, degradation was followed by BOD determinations.
The test item showed:
0.0 % degradation after 7 days
0.0 % degradation after 14 days
0.0 % degradation after 21 days
0.0 % degradation after 28 days
Therefore, the test item is considered to be "Not Readily Biodegradable".
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