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EC number: 269-616-7 | CAS number: 68307-94-8
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
The acute toxicity of the test item to the freshwater invertebrate Daphnia magna has been investigated and gave a 48-Hour EL50 (effective loading rate) value of greater than 100 mg/l loading rate WAF. The No Observed Effect Loading rate was 56 mg/l loading rate WAF.
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Effect concentration:
- 100 mg/L
Additional information
Introduction
A study was performed to assess the acute toxicity of the test item to Daphnia magna. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (April 2004) No 202, "Daphnia sp, Acute Immobilisation Test" referenced as Method C.2 of Commission Regulation (EC) No. 440/2008.
Methods
Following a preliminary range-finding test, twenty daphnids (2 replicates of 10 animals) were exposed to Water Accommodated Fractions (WAFs) of the test item over a range of nominal loading rates of 10, 18, 32, 56 and 100 mg/l for 48 hours at a temperature of 22°C to 23ºC under static test conditions. The number of immobilised Daphnia were recorded after 24 and 48 hours.
Results
The 48-Hour EL50(effective loading rate) for the test item to Daphnia magna based on nominal loading rates was greater than 100 mg/l loading rate WAF. The No Observed Effect Loading rate was 56 mg/l loading rate WAF.
Analysis of the 56 and 100 mg/l loading rate WAFs at 0 hours showed measured concentrations of 12.0 and 8.36 mg/l respectively. It was considered that the result for the 56 mg/l loading rate WAF was erroneous given the results from the 100 mg/l loading rate WAF. Analysis of the duplicate sample, stored at approximately -20°C prior to analysis, showed a measured concentration of 6.08 mg/l indicating the initial result was erroneous. Analysis of the 56 and 100 mg/l loading rate WAFs at 48 hours showed measured concentrations of 7.22 and 8.09 mg/l respectively.
The dissolved test item may have been one or several components of the test item. Given that toxicity cannot be attributed to a single component or mixture of components but to the test item as a whole, the results were based on nominal loading rates only.
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