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EC number: 269-616-7 | CAS number: 68307-94-8
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
The effect of the test item on the growth of Pseudokirchneriella subcapitata has been investigated over a 72-Hour period and gave the following results:
Growth rate:
EL50: >100 mg/l Loading Rate WAF
Yield:
EL50: 1.1 mg/l Loading Rate WAF
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 100 mg/L
- EC10 or NOEC for freshwater algae:
- 1 mg/L
Additional information
Introduction.
A study was performed to assess the effect of the test item on the growth of the green alga Pseudokirchneriella subcapitata. The method followed was designed to be compatible with the OECD Guidelines for Testing of Chemicals (2006) No 201, "Freshwater Alga and Cyanobacteria, Growth Inhibition Test" referenced as Method C.3 of Commission Regulation (EC) 761/2009.
Methods.
Following preliminary range-finding tests, Pseudokirchneriella subcapitata was exposed to Water Accommodated Fractions (WAFs) of the test item over a range of nominal loading rates of 1.0, 3.2, 10, 32 and 100 mg/l (three replicate flasks per concentration) for 72 hours, under constant illumination and shaking at a temperature of 24 ± 1°C.
Samples of the algal populations were removed daily and cell concentrations determined for each control and treatment group, using a Coulter Multisizer Particle Counter.
Results.
Exposure of Pseudokirchneriella subcapitata to the test item gave the following results:
Response Variable |
EL*50 |
No Observed Effect Loading Rate (NOEL) (mg/l) |
Lowest Observed Effect Loading Rate (LOEL) (mg/l) |
Growth Rate |
>100 |
<1.0 |
1.0 |
Yield |
1.1 |
<1.0 |
1.0 |
*EL = Effective Loading Rate
Chemical analysis of the test preparations at 0 hours showed measured test concentrations to range from 0.30 mg/l at 1.0 mg/l loading rate WAF through to 2.4 mg/l at 100 mg/l loading rate WAF. A decline in measured test concentration was observed at 72 hours in the range of less than the limit of quantitation (LOQ) of the analytical method employed (which was determined to be 0.031 mg/l) at 1.0 mg/l loading rate WAF through to 1.1 mg/l at 100 mg/l loading rate WAF.
Given that the toxicity cannot be attributed to a single component or a mixture of components but to the test item as a whole the results were based on nominal loading rates only.
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