Amides, C16-18, N-[3-(dimethylamino)propyl]

Toxicity to soil macroorganisms except arthropods

Description of key information

28 d NOEC (mortality, biomass and reproduction, Eisenia fetida) = 250 mg/kg soil DW; OECD guideline 222, RL1, GLP; read-across from C20/22 ATQ

Key value for chemical safety assessment

Long-term EC10, LC10 or NOEC for soil macroorganisms:

250 mg/kg soil dw

Additional information

No experimental data are available for C16-18 DMAPA amidoamine. However, studies are available with the source substance C20/22ATQ and with C22-trimethylammonium chloride. For justification for read-across see endpoint summary "Terrestrial toxicity".

Effects of C20/22 ATQ on mortality, biomass and reproduction of the earthworm species Eisenia fetida (Savigny) were determined according to the guidelines DIN ISO 11268-2 (1998), OECD 222 (2004) and BBA (1994). The study was conducted under static conditions over 8 weeks with three different application rates of 250 – 500 - 1000 mg/kg soil dry weight (DW), which were thoroughly mixed with artificial soil containing 10 % peat. Four replicates per control and test item concentration were examined. Replicates contained 10 test organisms with an individual weight of 0.30 to 0.60 g each.

After 28 days no significant mortality and no significant pathological symptoms or changes in the behaviour of adult earthworms were observed at all tested substance concentrations compared to the control. However, compared to the control the increase in body weight of the adult earthworms was significantly reduced at the highest test item concentration of 1000 mg/kg soil dry weight. After further four weeks the reproduction rate (average number of juveniles) came to 420 in the untreated control group and ranged between 211 and 391 in the test item groups. Compared to the control the reproduction was significantly reduced at the test item concentrations of 500 and 1000 mg/kg soil DW.

Under the conditions of this study, C20/22 ATQ had significant effects on biomass and reproduction of adult earthworms at the test item concentrations of 500 and 1000 mg/kg soil DW.

The No Observed Effect Concentration of C20/22 ATQ with respect to mortality, biomass and reproduction of Eisenia fetida was observed after 8 weeks of exposure to artificial soil (containing 10 % peat) at the application rate of 250 mg/kg soil DW.

 

The acute effects of C22-trimethylammonium chloride on the earthworm Eisenia fetida (Savigny 1826) were determined according to the OECD Guideline 207 and DIN ISO 11268-1 The study was conducted with the nominal concentration of 1000 mg test substance/kg soil dry weight (DW) over a time of 14 days. 40 test organisms with an individual weight between 0.30 - 0.60 g, divided into four replicates, were tested per concentration and control in artificial soil as described by the guideline. No significant mortality was observed at the limit test item concentration 1000 mg test substance/kg soil DW after 14 days of exposure. No pathological symptoms or changes in behaviour of the earthworms were seen neither in the control group nor in the tested limit concentration. Mean body weight loss during the study was below 20% in both groups and thus, biologically not significant.