Tetrasodium hydrogen 2-phosphonatobutane-1,2,4-tricarboxylate

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

11/93

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Qualifier:

according to guideline

Guideline:

other: "Prolonged Toxicity Study with Daphnia magna: Effects on Reproduction" (proposal for an update of OECD guideline 202, Part II; Draft 6/5/1991)

Deviations:

no

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Deviations:

yes

Remarks:

, the light intensity was slightly too high (25 instead of max. 20 µE/m2*s), CSB was determined.

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

The test concentrations were analysed at t= 0h at the start of the experiment (1993-11-03) and after 48h after start of the experiment.
After renewal of the medium at the third day (1993-11-05) of the experiment the test concentrations were analysed at t= 0h and after 72h after renewal of the medium.

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

- Daphnia magna Straus
- parthenogenetic females- Source: West-Aquarium (Bad Lauterberg; Germany)
- Strain: clone breeding of the Federal Health Office, Berlin
- Source: Laborzucht der Bayer AG, Leverkusen (in-house breeding, Bayer AG)
- Age: 6 - 24 hours

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Hardness:

Not indicated

Test temperature:

ca. 20°C +/-2°C; per single test: 20°C +/-1°C

pH:

Controls: 7.6 - 8.1
1000 mg/L: 7.9-8.6

Dissolved oxygen:

Test vessels containing controls (%): 98.5-121.2
Test vessels containing 1000 mg/L (%): 100.2-129.1

Salinity:

Not indicated

Nominal and measured concentrations:

Nominal concentrations in mg/L.: 10; 31.6; 100; 316; 1000
Measured concentrations in mg/L: 10-11; 31-34; 99-109; 318-337; 991-1159

Details on test conditions:

TEST SYSTEM
- Photoperiod (hrs dark / hrs light): 8 / 16 hours rhythm
- Light intensity: ca.25 µE/m2*s; 1700 Lux
- Test medium:100 mL, non-aerated
- Vehicle: no
No. of organisms per vessel:
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
Feed: algae "Scenedesmus subspicatus"

Reference substance (positive control):

no

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 1 071 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

immobilisation

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 329 - < 1 071 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

reproduction

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

104 mg/L

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: DUNNETT-TEST

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

329 mg/L

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: DUNNETT-TEST

Details on results:

The results are expressed in terms of the arithmetic mean of measured concentrations. Effective concentrations ranged from 98.1 to 115.9 % of nominal values during the experiment.

Reported statistics and error estimates:

The DUNETT test was performed for NOEC (reproduction) and LOEC (reproduction).

Validity criteria fulfilled:

yes

Conclusions:

EC 50 (immobilisation, 21d): > 1071 mg/L
EC 50 (reproduction, 21d): > 329 - < 1071 mg/L
NOEC (reproduction, 21d): 104 mg/L
LOEC (reproduction, 21d): 329 mg/L

Executive summary:

A study was performed to assess the effects of 2-phosphonobutane-1,2,4-tricarboxylic acidon the reproductive output of parthenogenetic Daphnia magna STRAUS under semi static conditions. The study was conducted in accordance with the proposal for an update of OECD guideline 202, Part II; Draft 6/5/1991: “Prolonged Toxicity Study with Daphnia magna: Effects on Reproduction" After a 21d-exposure period, the total number of living offspring produced per parent animal alive at the end of the test was assessed. The reproductive output of the Daphnia exposed to the test substance was compared to that of the control in order to determine the Lowest Observed Effect Concentration (LOEC) and the No Observed Effect Concentration (NOEC) according to the Dunnett-Test Procedure. A NOEC of 104 mg/l and a LOEC of 329 mg/l for the endpoint reproduction were obtained. The results relate to the arithmetic mean of measured concentrations. The stability of the substance during the experiment was confirmed by analytical monitoring. The recovery rate was between 98.1% and 115.9%.

This toxicity study is classified as acceptable and satisfies the guideline requirements for the chronic toxicity study to aquatic invertebrates (Daphnia magna).

Reference 2

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

11/93

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

comparable to guideline study

Justification for type of information:

Please find the Justification attached to Chapter 13.2.

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

other: "Prolonged Toxicity Study with Daphnia magna: Effects on Reproduction" (proposal for an update of OECD guideline 202, Part II; Draft 6/5/1991)

Deviations:

no

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

Deviations:

yes

Remarks:

, the light intensity was slightly too high (25 instead of max. 20 µE/m2*s), CSB was determined.

GLP compliance:

yes

Analytical monitoring:

yes

Details on sampling:

The test concentrations were analysed at t= 0h at the start of the experiment (1993-11-03) and after 48h after start of the experiment.
After renewal of the medium at the third day (1993-11-05) of the experiment the test concentrations were analysed at t= 0h and after 72h after renewal of the medium.

Vehicle:

no

Test organisms (species):

Daphnia magna

Details on test organisms:

- Daphnia magna Straus
- parthenogenetic females- Source: West-Aquarium (Bad Lauterberg; Germany)
- Strain: clone breeding of the Federal Health Office, Berlin
- Source: Laborzucht der Bayer AG, Leverkusen (in-house breeding, Bayer AG)
- Age: 6 - 24 hours

Test type:

semi-static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

21 d

Hardness:

Not indicated

Test temperature:

ca. 20°C +/-2°C; per single test: 20°C +/-1°C

pH:

Controls: 7.6 - 8.1
1000 mg/L: 7.9-8.6

Dissolved oxygen:

Test vessels containing controls (%): 98.5-121.2
Test vessels containing 1000 mg/L (%): 100.2-129.1

Salinity:

Not indicated

Nominal and measured concentrations:

Nominal concentrations in mg/L.: 10; 31.6; 100; 316; 1000
Measured concentrations in mg/L: 10-11; 31-34; 99-109; 318-337; 991-1159

Details on test conditions:

TEST SYSTEM
- Photoperiod (hrs dark / hrs light): 8 / 16 hours rhythm
- Light intensity: ca.25 µE/m2*s; 1700 Lux
- Test medium:100 mL, non-aerated
- Vehicle: no
No. of organisms per vessel:
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
Feed: algae "Scenedesmus subspicatus"

Reference substance (positive control):

no

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 1 071 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

immobilisation

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 329 - < 1 071 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

test mat.

Basis for effect:

reproduction

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

104 mg/L

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: DUNNETT-TEST

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

329 mg/L

Conc. based on:

test mat.

Basis for effect:

reproduction

Remarks on result:

other: DUNNETT-TEST

Details on results:

The results are expressed in terms of the arithmetic mean of measured concentrations. Effective concentrations ranged from 98.1 to 115.9 % of nominal values during the experiment.

Reported statistics and error estimates:

The DUNETT test was performed for NOEC (reproduction) and LOEC (reproduction).

Validity criteria fulfilled:

yes

Conclusions:

EC 50 (immobilisation, 21d): > 1071 mg/L
EC 50 (reproduction, 21d): > 329 - < 1071 mg/L
NOEC (reproduction, 21d): 104 mg/L
LOEC (reproduction, 21d): 329 mg/L

Executive summary:

As there is no data available for tetrasodium hydrogen 2-phosphonatobutane-1,2,4-tricarboxylate a read-across approach with the corresponding parent acid 2-phosphonobutane-1,2,4-tricarboxylic acid is proposed.

In aqueous media, tetrasodium hydrogen 2-phosphonatobutane-tricarboxylate and 2-phosphono-butane-1,2,4-tricarboxylic acid dissociate into the corresponding anion (2-phosphonatobutane-tricarboxylate ion) and the sodium ion and hydrogen ion (proton), respectively. Fate, behavior and the ecotoxicological properties of 2-phosphonobutane-1,2,4-tricarboxylic acid and its tetrasodium salt are thought to be an effect of the phosphonato-carboxylate ion rather than of the sodium ion or the hydrogen ion (proton), which are normal constituents in environmental systems and have no relevant ecotoxic properties in low concentrations.

Therefore a read-across between tetrasodium hydrogen 2-phosphonatobutane-tricarboxylate and 2-phosphonobutane-1,2,4-tricarboxylic acid is justified.

A study was performed to assess the effects of 2-phosphonobutane-1,2,4-tricarboxylic acidon the reproductive output of parthenogenetic Daphnia magna STRAUS under semi static conditions. The study was conducted in accordance with the proposal for an update of OECD guideline 202, Part II; Draft 6/5/1991: “Prolonged Toxicity Study with Daphnia magna: Effects on Reproduction" After a 21d-exposure period, the total number of living offspring produced per parent animal alive at the end of the test was assessed. The reproductive output of the Daphnia exposed to the test substance was compared to that of the control in order to determine the Lowest Observed Effect Concentration (LOEC) and the No Observed Effect Concentration (NOEC) according to the Dunnett-Test Procedure. A NOEC of 104 mg/l and a LOEC of 329 mg/l for the endpoint reproduction were obtained. The results relate to the arithmetic mean of measured concentrations. The stability of the substance during the experiment was confirmed by analytical monitoring. The recovery rate was between 98.1% and 115.9%.

This toxicity study is classified as acceptable and satisfies the guideline requirements for the chronic toxicity study to aquatic invertebrates (Daphnia magna).

Description of key information

As there is no data available for tetrasodium hydrogen 2-phosphonatobutane-1,2,4-tricarboxylate, results of the parent acid, 2-phosphonobutane-1,2,4-tricarboxylic acid are taken into account for this endpoint.
Prolonged toxicity with Daphnia magna was performed according to "Effects on Reproduction" (proposal for an update of OECD guideline 202, Part II; Draft 6/5/1991 covering OECD 211) and the following results were obtained:
EC 50 (immobilisation, 21d): > 1071 mg/L
EC 50 (reproduction, 21d): > 329 - < 1071 mg/L
NOEC (reproduction, 21d): 104 mg/L
LOEC (reproduction, 21d): 329 mg/L

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

NOEC

Remarks:

reproduction

Effect concentration:

104 mg/L

Additional information

As there is no data available for tetrasodium hydrogen 2-phosphonatobutane-1,2,4-tricarboxylate a read-across approach with the corresponding parent acid 2-phosphonobutane-1,2,4-tricarboxylic acid is proposed.

In aqueous media, tetrasodium hydrogen 2-phosphonatobutane-tricarboxylate and 2-phosphono-butane-1,2,4-tricarboxylic acid dissociate into the corresponding anion (2-phosphonatobutane-tricarboxylate ion) and the sodium ion and hydrogen ion (proton), respectively. Fate, behavior and the ecotoxicological properties of 2-phosphonobutane-1,2,4-tricarboxylic acid and its tetrasodium salt are thought to be an effect of the phosphonato-carboxylate ion rather than of the sodium ion or the hydrogen ion (proton), which are normal constituents in environmental systems and have no relevant ecotoxic properties in low concentrations.

Therefore a read-across between tetrasodium hydrogen 2-phosphonatobutane-tricarboxylate and 2-phosphonobutane-1,2,4-tricarboxylic acid is justified.

 

A study was performed to assess the effects of 2-phosphonobutane-1,2,4-tricarboxylic acid on the reproductive output of parthenogenetic Daphnia magna STRAUS under semi static conditions. The study was conducted in accordance with the proposal for an update of OECD guideline 202, Part II; Draft 6/5/1991: “Prolonged Toxicity Study with Daphnia magna: Effects on Reproduction" After a 21d-exposure period, the total number of living offspring produced per parent animal alive at the end of the test was assessed. The reproductive output of the Daphnia exposed to the test substance was compared to that of the control in order to determine the Lowest Observed Effect Concentration (LOEC) and the No Observed Effect Concentration (NOEC) according to the Dunnett-Test Procedure. A NOEC of 104 mg/l and a LOEC of 329 mg/l for the endpoint reproduction were obtained. The results relate to the arithmetic mean of measured concentrations. The stability of the substance during the experiment was confirmed by analytical monitoring. The recovery rate was between 98.1% and 115.9%.

This toxicity study is classified as acceptable and satisfies the guideline requirements for the chronic toxicity study to aquatic invertebrates (Daphnia magna).