Benzene, C15-16-alkyl derivs.

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

migrated information: read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

Nov. 3, 1998-Nov. 19, 1998

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: GLP guideline study for read-across source substance (European commercial linear alkyl benzene (LAB), CAS No. 67774-74-7) done according to OECD guideline 204.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1999

Report date:

1999

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Benzene, C10-13-alkyl derivs.

EC Number:

267-051-0

EC Name:

Benzene, C10-13-alkyl derivs.

Cas Number:

67774-74-7

IUPAC Name:

undecylbenzene

Constituent 2

Reference substance name:

Undecylbenzene

EC Number:

229-806-2

EC Name:

Undecylbenzene

Cas Number:

6742-54-7

IUPAC Name:

undecylbenzene

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Sampling was done on test solutions on days 1 and 14 of the test. Samples were taken from the test solution both before treatment, and after 24 hrs of treatment.

Test solutions

Details on test solutions:

1 g/L of LAB was added to reconstituted water; this was stirred for 24 hours and then transferred to a separatory funnel and allowed to stand 4 hours.  The lower portion was slowly filtered (0.45 um HV Millipore) and used for testing and for dilutions (2:1 and 1:1).

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Source: YARA Tropical Fish
- Length at study initiation (length definition, mean, range and SD): 3 cm average
- Weight at study initiation (mean and range, SD): 0.27 g average
- Feeding during test
- Food type: Mangime SERA DISKUS
- Amount: 2% per initial fish weight
- Frequency: daily


ACCLIMATION
- Acclimation period: 14 days
- Health during acclimation (any mortality observed): no mortality after 1st 48 hrs

Study design

Test type:

semi-static

Total exposure duration:

14 d

Test conditions

Hardness:

246 mg/L CaCO3

Test temperature:

24.2 degree C

pH:

7.4

Dissolved oxygen:

8.0 mg/L

Nominal and measured concentrations:

Mean measured concentration: before treatment (0 hrs) 5.53, 6.64, and 10.18 ug/L
after treatment (24 hrs) 0.43, 1.37, and 2.68 ug/L
Nominal concentration: 0, 5, 6.5, and 10 ug/L

Details on test conditions:

TEST SYSTEM
- Test vessel: glass beakers
- Material, size, headspace, fill volume: 5 L
- Renewal rate of test solution (frequency/flow rate): daily
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 2
- No. of vessels per control (replicates): 2
- No. of vessels per vehicle control (replicates):
- Biomass loading rate: 0.54 g fish/L


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water
- Ca/mg ratio: 4:1



OTHER TEST CONDITIONS
- Photoperiod: 16 hrs light/8 hrs dark
- Light intensity: artificial light


EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Daily observations were made of swimming behaviour, reaction to stimuli, discoloration, food intake, and mortality. All fish were weighed and measured before the test. Survivors were weighed at test termination.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

> 10 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Effect concentrations 2

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 10 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

No effects were observed for the concentrations tested. Both the LOEC and NOEC were > 10 ug/L. A decrease in the test substance concentration was observed within 24 hours (day 1 and day 14), but the daily renewal of test solutions guaranteed the exposure to the test substance.

Any other information on results incl. tables

Sublethal observations / clinical signs:

Body weight (mean)              

Concentration	0 (ug/L)	5 (ug/L)	7.5 (ug/L)	10 (ug/L)
Initial (g)	0.20	0.4	0.20	0.28
Final (g)	0.20	0.41	0.21	0.28

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The 14-day NOEC for fish was > 10 ug/L.

Executive summary:

In cases where no data were available on the target substance, Benzene, C15 -16 -alkyl derivs., data were read across from a structurally related material (the test substance).

No study regarding toxicity to fish was available for benzene, C15 -16 -alkyl derivs.; however, a valid 14 -day study performed according to OECD guideline 204 determined the long-term toxicity to fish of the read-across source substance (European commercial linear alkyl benzene (LAB)) . Fish were exposed for 14 days to a water accommodated fraction of 0, 5, 6.5, and 10 ug/L of test substance. The test medium was renewed daily. Fish were monitored daily for toxicity during the study. No adverse effects were seen during the study period. The NOEC for fish is > 10 ug/L. The LC50 was > 10 ug/L/day.