3,3'-(hexane-1,6-diyldiimino)dipropanenitrile

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Testing was conducted between 03 June 2009 and 10 July 2009

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Fully GLP and test guideline compliant study.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2009

Report date:

2009

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

3,3'-(hexane-1,6-diyldiimino)dipropanenitrile

EC Number:

700-129-9

Cas Number:

2004-62-8

Molecular formula:

C12H22N4

IUPAC Name:

3,3'-(hexane-1,6-diyldiimino)dipropanenitrile

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples were taken at the start and end of the exposure period.

Test solutions

Vehicle:

yes

Details on test solutions:

For the purpose of the definative test the test material was dissolved directly in reconstituted water.
200 mg of the test material was dissolved in 2 litres of water to give a 100 mg/l test concentration from which a series of dilutions was made to give further test concentrations of 58, 32, 10 and 100 mg/l.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The test was carried out using 1st instar Daphnia magna derived from in-house laboratory cultures.

Juveniles used for testing were less than 24 hours old.
Cultures were maintained in re-constituted water.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Hardness:

250 mg/l as CaCO3

Test temperature:

21 to 22oC

pH:

7.9 to 8.2

Dissolved oxygen:

7.9 to 8.7 mg O2/l (89 to 100 %ASV (Air Saturation Value))

Salinity:

Not applicablr

Nominal and measured concentrations:

Nominal test concentrations: 58, 32, 10 and 100 mg/l.
Measured test concentrations ranged from 82 to 106% of nominal value.

Details on test conditions:

Measured environmental values were all in expected ranges.

Reference substance (positive control):

yes

Remarks:

Potassium dichromate reference material.

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

63 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Remarks on result:

other: 95% CL 55-72 mg/l

Effect concentrations 2

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

32 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Results with reference substance (positive control):

The results from the positive control with potassium dichromate were within the normal range for this reference material. The mean 48-hour value calculated from all positive controls was 0.78 mg/l (sd = 0.21).

Reported statistics and error estimates:

The results were based on observation of the data.

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Executive summary:

The acute toxicity of the test material to the freshwater invrtebrate Daphnia magna has been investigated and gave a 48 -hour EC50 value of 63 mg/l with 95% confidence limites of 55 -72 mg/l. The No Observed Effect Concentration at 48 hours was 32 mg/l.