1,2-Naphtoquinonediazide-5-sulfonylchloride (or sulfonic acid) esters with 4,6-bis(1-(4-hydroxyphenyl)-1-methylethyl)-1,3-dihydroxybenzene

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

June 10, 2016 - June 13, 2016

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

No analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Principles of method if other than guideline:

Algae growth inhibition test

GLP compliance:

not specified

Remarks:

Study conducted in Japan, not for REACH purposes

Test material

Test material information

Constituent 1

Reference substance name:

4-[2-(2,4-dihydroxy-5-{2-[4-({[6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalen-1-yl]sulfonyl}oxy)phenyl]propan-2-yl}phenyl)propan-2-yl]phenyl 6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalene-1-sulfonate

Molecular formula:

C44H34N4O10S2

IUPAC Name:

4-[2-(2,4-dihydroxy-5-{2-[4-({[6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalen-1-yl]sulfonyl}oxy)phenyl]propan-2-yl}phenyl)propan-2-yl]phenyl 6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalene-1-sulfonate

Constituent 2

Reference substance name:

4-(2-{2,4-dihydroxy-5-[2-(4-hydroxyphenyl)propan-2-yl]phenyl}propan-2-yl)phenyl 6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalene-1-sulfonate

Molecular formula:

C34H30N2O7S

IUPAC Name:

4-(2-{2,4-dihydroxy-5-[2-(4-hydroxyphenyl)propan-2-yl]phenyl}propan-2-yl)phenyl 6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalene-1-sulfonate

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test item and medium were mixed to prepare each nominal concentration and stirred for 48 h in the preparation container under shading. Then the suspension was filtered with a glass fiber filter (GB-140, 0.4 µm pore size, Toyo Roshi) by suction to prepare the test solution. The test item was treated under yellow fluorescent light.
- Appearance of test solution: colorless without insoluble matter (at the start of exposure: visual)

Test organisms

Test organisms (species):

Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Study design

Test type:

not specified

Water media type:

freshwater

Remarks:

OECD medium

Limit test:

no

Total exposure duration:

72 h

Test conditions

Test temperature:

21-24°C

pH:

at the start: 7.9
at the end: 8.1

Nominal and measured concentrations:

Nominal: 1.0, 10, 100 mg/l and control

Details on test conditions:

TEST SYSTEM
- Test vessel: 300 mL erlenmeyer flask with gas-permeable silicon rubber plug
- Fill volume: 100 ml
- No. of vessels per concentration (replicates): 3

EFFECT PARAMETERS MEASURED
- Biomass: Chlorophyll fluorescence value was measured

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Duration:

72 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Basis for effect:

growth rate

Any other information on results incl. tables

Test Level (mg/L)	Growth Inhibition rate (%)
	(Growth Rate 0-3d)
Control	-----
1.0	2.9
10	-0.69
100	4.6