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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
June 10, 2016 - June 13, 2016
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
No analytical monitoring

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2016
Report date:
2016

Materials and methods

Principles of method if other than guideline:
Algae growth inhibition test
GLP compliance:
not specified
Remarks:
Study conducted in Japan, not for REACH purposes

Test material

Constituent 1
Chemical structure
Reference substance name:
4-[2-(2,4-dihydroxy-5-{2-[4-({[6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalen-1-yl]sulfonyl}oxy)phenyl]propan-2-yl}phenyl)propan-2-yl]phenyl 6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalene-1-sulfonate
Molecular formula:
C44H34N4O10S2
IUPAC Name:
4-[2-(2,4-dihydroxy-5-{2-[4-({[6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalen-1-yl]sulfonyl}oxy)phenyl]propan-2-yl}phenyl)propan-2-yl]phenyl 6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalene-1-sulfonate
Constituent 2
Chemical structure
Reference substance name:
4-(2-{2,4-dihydroxy-5-[2-(4-hydroxyphenyl)propan-2-yl]phenyl}propan-2-yl)phenyl 6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalene-1-sulfonate
Molecular formula:
C34H30N2O7S
IUPAC Name:
4-(2-{2,4-dihydroxy-5-[2-(4-hydroxyphenyl)propan-2-yl]phenyl}propan-2-yl)phenyl 6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalene-1-sulfonate

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test item and medium were mixed to prepare each nominal concentration and stirred for 48 h in the preparation container under shading. Then the suspension was filtered with a glass fiber filter (GB-140, 0.4 µm pore size, Toyo Roshi) by suction to prepare the test solution. The test item was treated under yellow fluorescent light.
- Appearance of test solution: colorless without insoluble matter (at the start of exposure: visual)

Test organisms

Test organisms (species):
Pseudokirchneriella subcapitata (previous names: Raphidocelis subcapitata, Selenastrum capricornutum)

Study design

Test type:
not specified
Water media type:
freshwater
Remarks:
OECD medium
Limit test:
no
Total exposure duration:
72 h

Test conditions

Test temperature:
21-24°C
pH:
at the start: 7.9
at the end: 8.1
Nominal and measured concentrations:
Nominal: 1.0, 10, 100 mg/l and control
Details on test conditions:
TEST SYSTEM
- Test vessel: 300 mL erlenmeyer flask with gas-permeable silicon rubber plug
- Fill volume: 100 ml
- No. of vessels per concentration (replicates): 3

EFFECT PARAMETERS MEASURED
- Biomass: Chlorophyll fluorescence value was measured
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Basis for effect:
growth rate

Any other information on results incl. tables

Test Level (mg/L) Growth Inhibition rate (%)
(Growth Rate 0-3d)
Control -----
1.0 2.9
10 -0.69
100 4.6

Applicant's summary and conclusion