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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
supporting study
Study period:
Start: 2016-06-06 End: 2016-06-08
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
no analytical monitoring

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2016
Report date:
2016

Materials and methods

Principles of method if other than guideline:
- Principle of test: 48-hour Immobilization test
- Short description of test conditions:
Test organism: Daphnia Magna( Clone A)
Exposure conditions
Exposure duration: 48 hours
Test type: static regime
Test concentration: 100, 10, 1 mg/L as nominal concentration
Environmental conditions
Dilution water: dechlorinated tap water
Temperature: 20°C +/- 1°
N° of organismes: 20 daphnids/test level

- Parameters analysed / observed:
Immobility
Dissolved oxygen
pH
GLP compliance:
not specified
Remarks:
Study conducted in Japan, not for REACH purposes

Test material

Constituent 1
Chemical structure
Reference substance name:
4-(2-{2,4-dihydroxy-5-[2-(4-hydroxyphenyl)propan-2-yl]phenyl}propan-2-yl)phenyl 6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalene-1-sulfonate
Molecular formula:
C34H30N2O7S
IUPAC Name:
4-(2-{2,4-dihydroxy-5-[2-(4-hydroxyphenyl)propan-2-yl]phenyl}propan-2-yl)phenyl 6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalene-1-sulfonate
Constituent 2
Chemical structure
Reference substance name:
4-[2-(2,4-dihydroxy-5-{2-[4-({[6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalen-1-yl]sulfonyl}oxy)phenyl]propan-2-yl}phenyl)propan-2-yl]phenyl 6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalene-1-sulfonate
Molecular formula:
C44H34N4O10S2
IUPAC Name:
4-[2-(2,4-dihydroxy-5-{2-[4-({[6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalen-1-yl]sulfonyl}oxy)phenyl]propan-2-yl}phenyl)propan-2-yl]phenyl 6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalene-1-sulfonate
Test material form:
solid
Details on test material:
Batch PW-9F-29

Test solutions

Vehicle:
no
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test item and dilution water were mixed to prepare each nominal concentration and stirred for 48 h under shading. Then the suspension was filtered with a glass fiber filter (GB-140, 0.4 µm pore size, Toyo Roshi) by suction to prepare the test solution. The test item was treated under yellow fluorescent light.
- Evidence of undissolved material: Appearance of test solution: colorless without insoluble matter (at the start of exposure: visual)

Test organisms

Test organisms (species):
Daphnia magna

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h

Test conditions

Test temperature:
20 ± 1 °C
pH:
7.8-7.9
Dissolved oxygen:
8.8-8.9
Nominal and measured concentrations:
nominal: 1.0, 10, 100 mg/l and control
Details on test conditions:
TEST SYSTEM
- Test vessel: 100mL glass beaker
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water


OTHER TEST CONDITIONS

- Light intensity: shading conditions

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility

Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility

Any other information on results incl. tables

 Test Level (mg/L)        Immobility (%)
24 hours 48 hours
Control 0
1.0  10 
10 5 15 
100  30 

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
EC50 > 100 mg/L