1,2-Naphtoquinonediazide-5-sulfonylchloride (or sulfonic acid) esters with 4,6-bis(1-(4-hydroxyphenyl)-1-methylethyl)-1,3-dihydroxybenzene

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

Start: 2016-06-06 End: 2016-06-08

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

guideline study with acceptable restrictions

Remarks:

no analytical monitoring

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2016

Report date:

2016

Materials and methods

Principles of method if other than guideline:

- Principle of test: 48-hour Immobilization test
- Short description of test conditions:
Test organism: Daphnia Magna( Clone A)
Exposure conditions
Exposure duration: 48 hours
Test type: static regime
Test concentration: 100, 10, 1 mg/L as nominal concentration
Environmental conditions
Dilution water: dechlorinated tap water
Temperature: 20°C +/- 1°
N° of organismes: 20 daphnids/test level

- Parameters analysed / observed:
Immobility
Dissolved oxygen
pH

GLP compliance:

not specified

Remarks:

Study conducted in Japan, not for REACH purposes

Test material

Test material information

Constituent 1

Reference substance name:

4-(2-{2,4-dihydroxy-5-[2-(4-hydroxyphenyl)propan-2-yl]phenyl}propan-2-yl)phenyl 6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalene-1-sulfonate

Molecular formula:

C34H30N2O7S

IUPAC Name:

4-(2-{2,4-dihydroxy-5-[2-(4-hydroxyphenyl)propan-2-yl]phenyl}propan-2-yl)phenyl 6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalene-1-sulfonate

Constituent 2

Reference substance name:

4-[2-(2,4-dihydroxy-5-{2-[4-({[6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalen-1-yl]sulfonyl}oxy)phenyl]propan-2-yl}phenyl)propan-2-yl]phenyl 6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalene-1-sulfonate

Molecular formula:

C44H34N4O10S2

IUPAC Name:

4-[2-(2,4-dihydroxy-5-{2-[4-({[6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalen-1-yl]sulfonyl}oxy)phenyl]propan-2-yl}phenyl)propan-2-yl]phenyl 6-(-lambda5-diazynylidene)-5-oxo-5,6-dihydronaphthalene-1-sulfonate

Test material form:

solid

Details on test material:

Batch PW-9F-29

Test solutions

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test item and dilution water were mixed to prepare each nominal concentration and stirred for 48 h under shading. Then the suspension was filtered with a glass fiber filter (GB-140, 0.4 µm pore size, Toyo Roshi) by suction to prepare the test solution. The test item was treated under yellow fluorescent light.
- Evidence of undissolved material: Appearance of test solution: colorless without insoluble matter (at the start of exposure: visual)

Test organisms

Test organisms (species):

Daphnia magna

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Test conditions

Test temperature:

20 ± 1 °C

pH:

7.8-7.9

Dissolved oxygen:

8.8-8.9

Nominal and measured concentrations:

nominal: 1.0, 10, 100 mg/l and control

Details on test conditions:

TEST SYSTEM
- Test vessel: 100mL glass beaker
- Aeration: no
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water


OTHER TEST CONDITIONS

- Light intensity: shading conditions

EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Any other information on results incl. tables

Test Level (mg/L)	Immobility (%)
	24 hours	48 hours
Control	0	0
1.0	0	10
10	5	15
100	0	30

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

EC50 > 100 mg/L