Registration Dossier
Registration Dossier
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EC number: 410-440-9 | CAS number: 164058-20-2
Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- 131984-21-9
- Cas Number:
- 131984-21-9
- IUPAC Name:
- 131984-21-9
- Details on test material:
- - Name of test material (as cited in study report): Dimethylbenzylidenecyclopentanone 95 %
- Lot/batch No.: COD-000462
- Analytical purity: 96.6 %
Constituent 1
Test solutions
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was added to deionized water and the mixture was stirred for 22±2 hours. Subsequently synthetic medium was added.
added and the aeration was started.
Test organisms
- Test organisms (species):
- activated sludge, domestic
- Details on inoculum:
- Activated sludge taken from a municipal wastewater treatment plant of the city Mannheim, Germany
- Initial biomass concentration: 1.0 g/l
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 180 min
Test conditions
- Test temperature:
- 20 ± 2 °C
- pH:
- 7.0 - 7.7
- Nominal and measured concentrations:
- Nominal test substance concentration:
999 mg/l - Details on test conditions:
- TEST SYSTEM
- Test vessel: Erlenmeyer vessel (250 ml)
- Fill volume: 250 ml
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 3
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: 8 mL/vessel 100-fold concentrated OECD medium - Reference substance (positive control):
- yes
- Remarks:
- 3,5 dichlorophenole
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 180 min
- Dose descriptor:
- other: EC20
- Effect conc.:
- > 999 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Duration:
- 180 min
- Dose descriptor:
- EC50
- Effect conc.:
- > 999 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Results with reference substance (positive control):
- EC20 (180 min)ca. 3.5 mg/l
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
