Registration Dossier
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EC number: 279-815-0 | CAS number: 81782-77-6
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
Key study: 96 h LC50 3 mg a.i./L, Pimephales promelas, OECD 203, EPA OPPTS 850.1075, Broughter et al. 2013a
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 3 mg/L
Additional information
The acute toxicity of the test material to fish was investigated in Pimephales promelas (fathead minnow) in a GLP study which was conducted in accordance with standardised guidelines OECD 203 and EPA OPPTS 850:1075. During the study the fish were exposed to the test material under flow through conditions at nominal test concentrations of 0.38, 0.75, 1.5, 3.0 and 6.0 mg a.i./L. The mean measured concentrations of test material ranged from 70 to 95% of the nominal values and were respectively 0.36, 0.65, 1.3, 2.5 and 4.2 mg a.i./L. All fish in the negative control, solvent control, 0.36 and 0.65 mg a.i./L treatment groups appeared normal throughout the test, with no mortalities or signs of acute toxicity observed. Percent mortality in the 2.5 and 4.2 mg a.i./L treatment groups at test termination was 15 and 100 %, respectively. Lethargy was observed among fish in the 1.3 mg a.i./L treatment group at the 96 hour interval. Signs of toxicity observed among fish in the 2.5 mg a.i./L treatment group included lying on the bottom test chamber, surfacing, lethargy, and loss of equilibrium. Fish were also observed lying on the bottom of the test chamber in the 4.2 mg a.i./L treatment group, prior to 100 % mortality in this group. Under the conditions of the study, the 96 hour LC50 value based on mean measured concentrations was 3.0 mg a.i./L, with a 95 % confidence interval of 2.5 to 4.2 mg a.i./L. The no mortality concentration was 1.3 mg a.i./L and the No Observed Effect Concentrations was 0.65 mg a.i./L.
The study was performed in line with GLP and accepted standardised guidelines with a high standard of reporting. The study was assigned a reliability score of 1 in accordance with the principles for assessing data quality as defined in Klimisch (1997).
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