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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

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Description of key information

The test substance is considered as 'not readily biodegradable' based on the results of a CO2 evolution test according to OECD Guideline 301B and EU Method C.4-C.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

A study was conducted to assess the ready biodegradability of the test substance according to OECD Guideline 301B and EU Method C.4-C, in compliance with GLP. The substance was evaluated at the equivalent of 20 mg organic carbon/ L (corresponding to 29.5 mg test substance/L) in test medium for 28 d. Activated sludge was used as inoculum (concentration: 25.0 mg dry matter/L). Aniline was chosen as positive control. The degradation was assessed in the form of CO2 evolution. Biodegradation of the test substance at the end of the study was 6%. In the toxicity control, a mean of 42% biodegradation was noted within 14 d of incubation. The test substance was not inhibitory to activated sludge at the applied concentration level. All validity criteria were met. Under the study conditions, the test substance was considered as 'not readily biodegradable' (Muckle M, 2015a).