p-tolyl acetate

Endpoint summary

Description of key information

Skin Irritation:

The p-tolyl acetate (CAS No. 140-39-6) tested at 4% in petrolatum produced no irritation after a 48-hr closed-patch test in 25 human subjects.

Thus, based on the human experience data it was concluded that p-tolyl acetate (CAS No. 140-39-6) was non-Irritating to the skin of human subjects under the experimental conditions tested and being classified as “Category- Not Classified” as per CLP Regulation.

Eye Irritation:

The ocular irritation potential of p-tolyl acetate (CAS No: 140-39-6) was estimated using OECD QSAR toolbox version 3.4 with log Kow as the primary descriptor. p-tolyl acetate was estimated to be not irritating to the eyes of rabbits.Based on the estimated result, p-tolyl acetate (CAS No: 140-39-6) can be considered to be notirritating to the eyes and being classified as "Unclassified" as per CLP regulation.

Key value for chemical safety assessment

Skin irritation / corrosion

Link to relevant study records

Reference

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

data is from peer-reviewed journal

Qualifier:

no guideline available

Principles of method if other than guideline:

Skin irritation potentials of p-tolyl acetate (CAS No. 140-39-9) were investigated in rabbits.

GLP compliance:

not specified

Specific details on test material used for the study:

Name of test material (as cited in study report): p-cresyl acetate
Molecular formula: C9H10O2
Molecular weight: 150.176 g/mol
Substance Type: Organic
Physical State: Liquid

Species:

rabbit

Strain:

not specified

Details on test animals or test system and environmental conditions:

no data available

Type of coverage:

not specified

Preparation of test site:

other: intact and abraded

Vehicle:

unchanged (no vehicle)

Controls:

not specified

Amount / concentration applied:

full strength

Duration of treatment / exposure:

no data available

Observation period:

no data available

Number of animals:

no data available

Details on study design:

no data available

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

other: no data available

Score:

0

Reversibility:

not specified

Remarks on result:

no indication of irritation

Irritant / corrosive response data:

No irritation was observed.

Interpretation of results:

other: not irritating

Conclusions:

The substance p-tolyl acetate (CAS No.140-39-6) when administered to rabbits by the dermal route, is to be considered ―non-irritant for the skin.

Executive summary:

Skin irritation potentials of p-tolyl acetate (CAS No. 140-39-9) were investigated in rabbits. p-tolyl acetate (CAS No.140-39-6) applied full strength on intact or abraded rabbit skin and effects were observed (duration not specified), produced no skin irritation. Hence, p-tolyl acetate (CAS No.140-39-6) was classified as non-irritating to skin.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Eye irritation

Link to relevant study records

Reference

Endpoint:

eye irritation: in vivo

Type of information:

(Q)SAR

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

results derived from a valid (Q)SAR model and falling into its applicability domain, with limited documentation / justification

Justification for type of information:

Data is from OECD QSAR toolbox v3.4 and the supporting QMRF report has been attached.

Qualifier:

no guideline available

Principles of method if other than guideline:

Prediction is done using OECD QSAR Toolbox version 3.4 with respect to the descriptor log Kow.

GLP compliance:

not specified

Specific details on test material used for the study:

Name of test material (as cited in study report): 4-methylphenyl acetate
Molecular formula: C9H10O2
Molecular weight: 150.176 g/mol
Substance Type: Organic
Physical State: Liquid

Species:

rabbit

Strain:

not specified

Details on test animals or tissues and environmental conditions:

no data available

Vehicle:

not specified

Controls:

not specified

Amount / concentration applied:

0.1 ml

Duration of treatment / exposure:

7 days

Observation period (in vivo):

7 days

Duration of post- treatment incubation (in vitro):

no data available

Number of animals or in vitro replicates:

3

Details on study design:

no data available

Irritation parameter:

overall irritation score

Basis:

mean

Time point:

7 d

Score:

0

Reversibility:

not specified

Remarks on result:

no indication of irritation

Irritant / corrosive response data:

No indication of irritation.

Other effects:

no data available

Estimation method: Takes mode value from the 5 nearest neighbours
Domain  logical expression:Result: In Domain

(((((((("a" or "b" or "c" or "d" or "e" )  and ("f" and ( not "g") )  )  and ("h" and ( not "i") )  )  and ("j" and ( not "k") )  )  and ("l" and ( not "m") )  )  and ("n" and ( not "o") )  )  and "p" )  and ("q" and "r" )  )

Domain logical expression index: "a"

Referential boundary: The target chemical should be classified as Esters (Acute toxicity) by US-EPA New Chemical Categories

Domain logical expression index: "b"

Referential boundary: The target chemical should be classified as Acetoxy AND Alkyl arenes AND Aryl AND Carboxylic acid ester by Organic Functional groups

Domain logical expression index: "c"

Referential boundary: The target chemical should be classified as Alkyl arenes AND Carboxylic acid ester AND Overlapping groups by Organic Functional groups (nested)

Domain logical expression index: "d"

Referential boundary: The target chemical should be classified as Aliphatic Carbon [CH] AND Aliphatic Carbon [-CH2-] AND Aliphatic Carbon [-CH3] AND Aromatic Carbon [C] AND Carbonyl, aliphatic attach [-C(=O)-] AND Ester, aromatic attach [-C(=O)O] AND Miscellaneous sulfide (=S) or oxide (=O) AND Olefinic carbon [=CH- or =C<] AND Oxygen, one aromatic attach [-O-] AND Oxygen, two olefinic attach [-O-] by Organic functional groups (US EPA)

Domain logical expression index: "e"

Referential boundary: The target chemical should be classified as Aromatic compound AND Carbonic acid derivative AND Carboxylic acid derivative AND Carboxylic acid ester by Organic functional groups, Norbert Haider (checkmol)

Domain logical expression index: "f"

Referential boundary: The target chemical should be classified as AN2 AND AN2 >> Shiff base formation after aldehyde release AND AN2 >> Shiff base formation after aldehyde release >> Specific Acetate Esters AND SN1 AND SN1 >> Nucleophilic attack after carbenium ion formation AND SN1 >> Nucleophilic attack after carbenium ion formation >> Specific Acetate Esters AND SN2 AND SN2 >> Acylation AND SN2 >> Acylation >> Specific Acetate Esters AND SN2 >> Nucleophilic substitution at sp3 Carbon atom AND SN2 >> Nucleophilic substitution at sp3 Carbon atom >> Specific Acetate Esters by DNA binding by OASIS v.1.4

Domain logical expression index: "g"

Referential boundary: The target chemical should be classified as AN2 >>  Michael-type addition, quinoid structures OR AN2 >>  Michael-type addition, quinoid structures >> Quinoneimines OR AN2 >> Michael-type addition on alpha, beta-unsaturated carbonyl compounds OR AN2 >> Michael-type addition on alpha, beta-unsaturated carbonyl compounds >> Four- and Five-Membered Lactones OR AN2 >> Michael-type conjugate addition to activated alkene derivatives OR AN2 >> Michael-type conjugate addition to activated alkene derivatives >> Alpha-Beta Conjugated Alkene Derivatives with Geminal Electron-Withdrawing Groups OR AN2 >> Nucleophilic addition to alpha, beta-unsaturated carbonyl compounds OR AN2 >> Nucleophilic addition to alpha, beta-unsaturated carbonyl compounds >> Alpha, Beta-Unsaturated Aldehydes OR AN2 >> Schiff base formation OR AN2 >> Schiff base formation >> Alpha, Beta-Unsaturated Aldehydes OR AN2 >> Schiff base formation by aldehyde formed after metabolic activation OR AN2 >> Schiff base formation by aldehyde formed after metabolic activation >> Geminal Polyhaloalkane Derivatives OR AN2 >> Shiff base formation for aldehydes OR AN2 >> Shiff base formation for aldehydes >> Haloalkane Derivatives with Labile Halogen OR No alert found OR Non-covalent interaction OR Non-covalent interaction >> DNA intercalation OR Non-covalent interaction >> DNA intercalation >> DNA Intercalators with Carboxamide and Aminoalkylamine Side Chain OR Non-covalent interaction >> DNA intercalation >> Organic Azides OR Radical OR Radical >> Radical mechanism by ROS formation OR Radical >> Radical mechanism by ROS formation >> Organic Azides OR Radical >> Radical mechanism via ROS formation (indirect) OR Radical >> Radical mechanism via ROS formation (indirect) >> Geminal Polyhaloalkane Derivatives OR Radical >> Radical mechanism via ROS formation (indirect) >> Nitroarenes with Other Active Groups OR Radical >> Radical mechanism via ROS formation (indirect) >> Nitrophenols, Nitrophenyl Ethers and Nitrobenzoic Acids OR Radical >> Radical mechanism via ROS formation (indirect) >> p-Substituted Mononitrobenzenes OR Radical >> Radical mechanism via ROS formation (indirect) >> Single-Ring Substituted Primary Aromatic Amines OR Radical >> Radical mechanism via ROS formation (indirect) >> Thiols OR Radical >> ROS formation after GSH depletion (indirect) OR Radical >> ROS formation after GSH depletion (indirect) >> Quinoneimines OR SN1 >> Carbenium ion formation OR SN1 >> Carbenium ion formation >> Alpha-Haloethers OR SN1 >> Nucleophilic attack after diazonium or carbenium ion formation OR SN1 >> Nucleophilic attack after diazonium or carbenium ion formation >> Nitroarenes with Other Active Groups OR SN1 >> Nucleophilic attack after nitrene formation OR SN1 >> Nucleophilic attack after nitrene formation >> Organic Azides OR SN1 >> Nucleophilic attack after nitrenium ion formation OR SN1 >> Nucleophilic attack after nitrenium ion formation >> Single-Ring Substituted Primary Aromatic Amines OR SN1 >> Nucleophilic attack after reduction and nitrenium ion formation OR SN1 >> Nucleophilic attack after reduction and nitrenium ion formation >> Nitroarenes with Other Active Groups OR SN1 >> Nucleophilic attack after reduction and nitrenium ion formation >> Nitrophenols, Nitrophenyl Ethers and Nitrobenzoic Acids OR SN1 >> Nucleophilic attack after reduction and nitrenium ion formation >> p-Substituted Mononitrobenzenes OR SN2 >> Acylation involving a leaving group  OR SN2 >> Acylation involving a leaving group  >> Haloalkane Derivatives with Labile Halogen OR SN2 >> Acylation involving a leaving group after metabolic activation OR SN2 >> Acylation involving a leaving group after metabolic activation >> Geminal Polyhaloalkane Derivatives OR SN2 >> Alkylation OR SN2 >> Alkylation >> Alkylphosphates, Alkylthiophosphates and Alkylphosphonates OR SN2 >> Alkylation, direct acting epoxides and related OR SN2 >> Alkylation, direct acting epoxides and related >> Epoxides and Aziridines OR SN2 >> Alkylation, nucleophilic substitution at sp3-carbon atom OR SN2 >> Alkylation, nucleophilic substitution at sp3-carbon atom >> Haloalkane Derivatives with Labile Halogen OR SN2 >> Alkylation, nucleophilic substitution at sp3-carbon atom >> Sulfonates and Sulfates OR SN2 >> Alkylation, ring opening SN2 reaction OR SN2 >> Alkylation, ring opening SN2 reaction >> Four- and Five-Membered Lactones OR SN2 >> Direct acting epoxides formed after metabolic activation OR SN2 >> Direct acting epoxides formed after metabolic activation >> Quinoline Derivatives OR SN2 >> Nucleophilic substitution at sp3 carbon atom after thiol (glutathione) conjugation OR SN2 >> Nucleophilic substitution at sp3 carbon atom after thiol (glutathione) conjugation >> Geminal Polyhaloalkane Derivatives OR SN2 >> SN2 at an activated carbon atom OR SN2 >> SN2 at an activated carbon atom >> Quinoline Derivatives OR SN2 >> SN2 at sp3-carbon atom OR SN2 >> SN2 at sp3-carbon atom >> Alpha-Haloethers OR SN2 >> SN2 attack on activated carbon Csp3 or Csp2 OR SN2 >> SN2 attack on activated carbon Csp3 or Csp2 >> Nitroarenes with Other Active Groups by DNA binding by OASIS v.1.4

Domain logical expression index: "h"

Referential boundary: The target chemical should be classified as (!Undefined)Group All Lipid Solubility < 0.01 g/kg by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "i"

Referential boundary: The target chemical should be classified as (!Undefined)Group CN Lipid Solubility < 0.4 g/kg OR (!Undefined)Group CNHal Lipid Solubility < 400 g/kg OR Group All Aqueous Solubility < 0.000005 g/L OR Group All Aqueous Solubility < 0.00002 g/L OR Group All log Kow > 9 OR Group All Melting Point > 200 C OR Group C Aqueous Solubility < 0.0001 g/L OR Group C Aqueous Solubility < 0.0005 g/L OR Group C Melting Point > 55 C OR Group C Molecular Weight > 380 g/mol OR Group CHal log Kow > 4.5 OR Group CHal Melting Point > 65 C OR Group CHal Molecular Weight > 280 g/mol OR Group CHal Molecular Weight > 370 g/mol OR Group CN Aqueous Solubility < 0.1 g/L OR Group CN Molecular Weight > 290 g/mol by Eye irritation/corrosion Exclusion rules by BfR

Domain logical expression index: "j"

Referential boundary: The target chemical should be classified as No alert found by in vitro mutagenicity (Ames test) alerts by ISS

Domain logical expression index: "k"

Referential boundary: The target chemical should be classified as alpha,beta-unsaturated aliphatic alkoxy group by in vitro mutagenicity (Ames test) alerts by ISS

Domain logical expression index: "l"

Referential boundary: The target chemical should be classified as Group 14 - Carbon C AND Group 16 - Oxygen O by Chemical elements

Domain logical expression index: "m"

Referential boundary: The target chemical should be classified as Group 15 - Nitrogen N OR Group 15 - Phosphorus P OR Group 17 - Halogens Br OR Group 17 - Halogens F,Cl,Br,I,At by Chemical elements

Domain logical expression index: "n"

Referential boundary: The target chemical should be classified as Aromatic compound AND Carbonic acid derivative AND Carboxylic acid derivative AND Carboxylic acid ester by Organic functional groups, Norbert Haider (checkmol)

Domain logical expression index: "o"

Referential boundary: The target chemical should be classified as Carboxylic acid anhydride OR Dialkylether OR Ether OR Heterocyclic compound by Organic functional groups, Norbert Haider (checkmol)

Domain logical expression index: "p"

Similarity boundary:Target: Cc1ccc(OC(C)=O)cc1
Threshold=30%,
Dice(Atom centered fragments)
Atom type; Count H attached; Hybridization

Domain logical expression index: "q"

Parametric boundary:The target chemical should have a value of log Kow which is >= 1.74

Domain logical expression index: "r"

Parametric boundary:The target chemical should have a value of log Kow which is <= 2.57

Interpretation of results:

other: not irritating

Conclusions:

p-tolyl acetate was estimated to be not irritating to the rabbit eyes.

Executive summary:

The ocular irritation potential of p-tolyl acetate (CAS No: 140-39-6) was estimated using OECD QSAR toolbox version 3.4 with log Kow as the primary descriptor. p-tolyl acetate was estimated to be not irritating to the eyes of rabbits. Based on the estimated result, p-tolyl acetate (CAS No: 140-39-6) can be considered to be not irritating to the eyes and being classified as "Unclassified" as per CLP regulation.

Endpoint conclusion

Endpoint conclusion:

no adverse effect observed (not irritating)

Respiratory irritation

Endpoint conclusion

Endpoint conclusion:

no study available

Additional information

Skin Irritation:

In different studies, p-tolyl acetate has been investigated for potential for dermal irritation to a greater or lesser extent. The studies are based on in vivo experiments in rabbits along with human data for target chemical p-tolyl acetate and its functionally similar read across substances benzyl propionate (CAS No. -122-63-4) and benzyl acetate(CAS: 140-11-4). The predicted data using the OECD QSAR toolbox has also been compared with the experimental data.

 

Various studies for p-tolyl acetate were summarized in Food and Cosmetics Toxicology, Volume 12, Issue 3, June 1974, Page 391 to assess the irritation in humans and rabbits.

p-tolyl acetate applied at full strength on intact or abraded rabbit skin, produced no irritation. Also, when tested in 25 human subjects at 4% in petrolatum in a 48-hr closed-patch test, p-tolyl acetate produced no irritation.

 

In a prediction done by SSS (2017), the skin irritation potential of p-tolyl acetate (CAS No: 140-39-6) was estimated using OECD QSAR toolbox version 3.4 with log Pow as the primary descriptor and considering the seven closest read across substances. The substance p-tolyl acetate was estimated to be not irritating to the skin of New Zealand White rabbits. 

 

Also these results are further supported by the experimental study conducted in an OECD GLP laboratory by Sustainability Support Services (Europe) AB for the functionally similar read across substance benzyl Propionate (CAS: 122-63-4).This study was performed as per OECD guideline No. 404. Three healthy young adult female rabbits were used for conducting acute dermal irritation study. Body weights were re­corded on day 0 (prior to application) and at termination. Rabbits with good intact skin were selected for the study. The hairs of all the rabbits were clipped at contralateral sites, approximately 24 hours prior to treatment. A dose of0.5 ml of test item (as such) was applied to the skin, over an area of approximately 6 x 6 cm clipped of hair on one side of rabbits. The other untreated side was kept as control area and 0.5 ml of distilled water was applied at this site. At the end of 4 hours, the gauze patch was removed and test item application site was wiped with water without altering the integrity of the epidermis. Initially, the test item was applied to the clipped area of skin of one rabbit. The test site was covered with gauze patch. After 4 hours of exposure in Animal No. 1, there was no erythema and oedema observed at 1, 24, 48 and 72 hours observation. Hence the confirmatory test was conducted on additional two rabbits (No. 2 and 3)to confirm the non irritant nature of the test item. In Animals No. 2 and 3 after post patch removal, revealed no erythema and oedema at 1, 24, 48 and 72 hours observation. The patch was removed after 4 hours and rabbits were observed for erythema and oedema at 1, 24, 48 and 72 hours after patch removal, evaluated and graded as per Draize method. The individual mean score at 24, 48 and 72 hours for Animal Nos. 1, 2 and 3 were 0.00, 0.00, 0.00 and 0.00, 0.00, 0.00, for erythema and oedema formation, respectively.  

Hence, it was concluded that “Benzyl propionate (CAS No. - 122 -63-4)” was Non-Irritating to the skin of Female New Zealand White rabbits under the experimental conditions tested.

 

The above results are further supported by the experimental study performed by P.J Frosch, et al., (Contact Dermatitis, vol 33,Pages 333-342,1995) for the functionally similar read across substance benzyl Acetate (CAS: 140-11-4). The patch test was performed on human subjects to assess the irritation potential of benzyl acetate (CAS No: 140 -11 -4). One hundred patients were patch tested with 1 or 5% benzyl acetate in petrolatum, using Finn Chambers on Scanpor applied for 2 days to the back. Scoring was done on the day the patch was removed and 1 or 2 days later. A weak positive response was seen in one patient given the 1% concentration. There were no effects in the 5% group. Thus, based on the human experience data it was concluded that benzyl acetate (CAS No. 140-11-4) was non-Irritating to the skin of human subjects under the experimental conditions tested.

On the basis of human and animal data for the target as well as it read across substances, it was concluded that p-tolyl acetate (CAS No. 140-39-6) was non-Irritating to the skin of human subjects and animals under the experimental conditions tested and being classified as “Category- Not Classified” as per CLP Regulation.

 

Eye Irritation: 

In different studies, p-tolyl acetate has been investigated for potential for ocular irritation to a greater or lesser extent. The studies are based on in vivo experiments in rabbits along with predicted data for target chemical p-tolyl acetate and its functionally similar read across substances benzyl propionate (CAS No. - 122-63-4) and n-butylacetate (CAS: 123-86-4). The predicted data using the OECD QSAR toolbox has also been compared with the experimental data.

The ocular irritation potential of p-tolyl acetate (CAS No: 140-39-6) was estimated by SSS(2017) using OECD QSAR toolbox version 3.4 with log Kow as the primary descriptor. p-tolyl acetate was estimated to be not irritating to the eyes of rabbits.

 

Also the above study is supported by the Acute Eye Irritation/Corrosion Study of functionally similar read across substance benzyl propionate (CAS no. - 122-63-4) in Rabbits, conducted by Sustainability Support Services (Europe) AB at SA-FORD (Sanctuary for Research and Development), Maharashtra, India. This study was performed as per OECD guideline No. 405.

Rabbits free from injury of eye were selected for the study. The eyes of all the rabbits were examined 24 hours prior to treatment. One eye of each rabbit served as control and other as treated. Control eye was left untreated whereas;0.1 ml of test item (as such)was instilled in the other (treated) eye of rabbits.The eye was observed at 1, 24, 48, 72 hours after test item instillation.Ophthalmoscope was used for scoring of eye lesions.

In the initial test,0.1 ml of test itemwas applied into the conjunctival sac of the right eye of Animal No.1. The left eye of the rabbit served as the control. Animal No. 1 presented ocular lesions at 1 hour observation period. Hence the confirmatory test was conducted on additional two rabbits (Animal No. 2 and 3);0.1 ml of test itemwas instilled into the conjunctival sac of right eye and left eye served as the control. Ocular lesions were observed at 1, 24 and 48 hour in Animal Number 2 whereas in Animal Number 3 ocular lesions presented only at 1 hour observation period.

Untreated eye of the treated rabbits was normal throughout the experimental period of 72 hours.

The following grading scores were observed in treated eye of tested rabbits.

Observation at 1 hour after instillation of test item revealed: Cornea-No ulceration or opacity in all 3 animals; Area of Opacity-Zero in all the animals;Iris:Normal in all the animals.Conjunctivae -Some blood vessels definitely hyperaemic (injected) in all the animals;Chemosis:Some swelling above normal (includes nictating membranes) were observed in animal number 1 and 3 whereas animal number 2 was normal.

Observation at 24 hours after instillation of test item revealed: Cornea-No ulceration or opacity in all the animals; Area of Opacity-Zero in all the animals;Iris:Normal in all the animals.Conjunctivae -Some blood vessels definitely hyperaemic (injected) was observed in animal no. 2. Animal no. 1 and 3 recovered to normal;Chemosis:No swelling was observed in all the Animals.

At 24 hours observation the rabbits were examined for corneal epithelium cell damage using sodium fluorescein strips and noticed 0 % damage in Animal Nos 1, 2 and 3, respectively.

Observation at 48 and 72 hours after instillation of test item revealed: Cornea-No ulceration or opacity in all the animals; Area of Opacity-Zero in all the animals;Iris:Normal in all the animals.Conjunctivae -Some blood vessels definitely hyperaemic (injected) was observed in animal no. 3 at 48 hours which was recovered to normal at 72 hours observation whereas blood vessels were normal in Animal Numbers 1 and 3 at 48 and 72 hours observation;Chemosis:No swelling was observed in all the animals. 

The individual mean score for Animal Nos. 1, 2 and 3at 24, 48, 72 hoursfor Corneal opacity, iris, conjunctiva, chemosis were found 0.00, 0.00, 0.00, 0.00 ; 0.00, 0.00, 0.67, 0.00 and 0.00, 0.00, 0.00, 0.00, respectively.

Under the experimental conditions tested, eye irritation and reversibility of effects on eyes of rabbits was observed at 72 hours. 

Hence under the experimental test conditions, Benzyl propionate (CAS No. - 122-63-4) is “Non Irritant” to New Zealand White Male rabbit eyes.

 

In addition to these studies, the experimental study was conducted by ECETOC (ECETOC Technical Report no. 48 (2), 1998) for the functionally similar read across substance n-butyl acetate (CAS: 123-86-4). Study was carried out according to OECD 405 “EYE IRRITATION” Guidelines. 0.1 ml of n-butyl acetate was instilled into the conjunctival sac of 4 New Zealand White rabbits. Observations were made after 1 hour, 4 hours and then 1, 2, 3 and 7 days after instillation of test chemical. The scoring was done according to the Draize method. Conjunctival redness and chemosis was observed in all animals after 1 day of observation. The MMAS (Maximum Modified Average Score) of n-butyl acetate was 7.5 after 1 day of observation. The effects were recovered fully after 7 days and the MMAS scores were 0.0.

Based on the classification of chemicals according to MMAS scores, n-butyl acetate can be considered to be not irritating to rabbit eyes

 

Moreover, these studies are further supported by the experimental study summarized in Concise International Chemical Assessment Document 64 - BUTYL ACETATES, World Health Organization, Geneva, 2005,for the functionally similar read across substance n-butyl acetate (CAS: 123-86-4). 0.1 ml of n-butyl acetate (123-86-4) was instilled into the eyes of 6 rabbits and the effects were observed. Iritis and minor to moderate conjunctivitis were observed which healed fully within 48 hours, but no corneal damage was observed. A maximum Draize score of 14.7 /110 (occurring at 4 hours) was noted when n-butyl acetate was tested in rabbits. Based on the Draize scores, n-butyl acetate can be considered to be not irritating to eyes.

 

On the basis of the above available data for the target as well as it read across substances; and by applying the weight of evidence approach, it can be concluded that p-tolyl acetate (CAS No: 140-39-6) can be considered to be not irritating to the eyes and being classified as "Unclassified" as per CLP regulation.

Justification for classification or non-classification

According to CLP Regulation EC No. 1272/2008 and based on the studies of skin and eye irritation, it is concluded that the substance p-tolyl acetate is not classified as skin and eye irritant.