[1,1'-Bianthracene]-9,9',10,10'-tetrone, 4,4'-bis[[4,6-bis[[3-(diethylamino)propyl]amino]-1,3,5-triazin-2-yl]amino]-

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

Jan 2014 - Feb 2015

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: Study conducted according to OECD guideline under GLP

Cross-reference

Cross-reference 1

Reason / purpose for cross-reference:

reference to same study

Cross-reference 2

Reason / purpose for cross-reference:

reference to other study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2015

Report date:

2015

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Principles of method if other than guideline:

Not relevant

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

308 AQN

IUPAC Name:

308 AQN

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Name of test material (as cited in study report): 308 AQN

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
PHYSICO-CHEMICAL PROPERTIES
- Water solubility (under test conditions): Poorly water soluble

Sampling and analysis

Analytical monitoring:

no

Details on sampling:

- Concentrations: 1000 mg/L
The test item was introduced into the test solution on one occasion.

Test solutions

Vehicle:

no

Details on test solutions:

The test item was tested for toxicity towards Daphnia magna STRAUS at different concentrations
(aqueous eluates/”WAF”2) from different amounts of the poorly water soluble test item) dissolved in
medium M4 acc. Elendt, as physiological conditions for the daphnids are necessary after the
preparation of the test solutions.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

The in-house cultivation of the daphnids is performed so that the animals are transferred into new
test medium (see below) at an interval of 1 to 3 days followed by feeding with Desmodesmus
subspicatus- and/or Chlorella sp.cells. Daphnids used for the test were less than 24 hours old. In
order to ensure the correct age, daphnids are transferred into fresh medium one day before the
study start in order to ensure the test animals were less than 24 hours old. Daphnids were not fed
during the study.

Study design

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Post exposure observation period:

not performed

Test conditions

Test temperature:

21.5 (t0) - 20.6 (48h) °C

pH:

9.28 (t0) - 7.95 (48h)

Dissolved oxygen:

6.3 (t0) - 7.0 (48h) mg/L

Nominal and measured concentrations:

No analytical method was found to be suitable for quantifying the test item in the aqueous phase.
Based on this, the reported effect concentrations (EL values) should refer to nominal
concentrations of the test item (as “WAF”).

Details on test conditions:

The test item was applied as follows: The test item was tested for toxicity towards daphnia as aqueous extracts (“WAF”) from up to 1000 mg test item/L (final nominal concentration in the main test). For this purpose, different amounts of the test item were dispersed
in dilution water. The elution of the water soluble part (WAF) of the test item took place on a shaking machine at 130 rpm speed over 18 hr3) at room
temperature, respectively. During that time an equilibration between the test item and the water was considered to be achieved. Thereafter, the
suspensions were filtered through glass fibre filters and membrane filters in order to eliminate nondissolved parts of the test item. The eluates were
used for the test without further dilution steps.
Four parallels were prepared for the test treatment with the test item and for the control without anytest item, respectively, using 5 animals each
parallel (main test). Two parallels were prepared for each concentration and the control without any test item, respectively, using 5 animals each
parallel in the screening test.

Reference substance (positive control):

yes

Remarks:

potassium dichromate

Results and discussion

Effect concentrations

Duration:

48 h

Dose descriptor:

EL50

Effect conc.:

> 1 000

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

Under the conditions used for the test, no immobilisation of the daphnids was observed at concentrations representing the water
soluble part (hereafter abbreviated “WAF”, water accommodated fraction) of the test item at
concentrations up to 1000 mg/L (only screening-test). The main test was performed as a limit test using the WAF from 1000 mg/L “308 AQN”.

Results with reference substance (positive control):

The value of the EL50 (24 hour) in the period of the present test was 0.81 mg/L potassium dichromate. This value is slightly lower than the range
of 0.9-1.9 indicated in the study plan but fully in accordance with the recommendations given in OECD Guideline 202 (Daphnia sp., Acute
Immobilisation Test) being in the range 0.6 – 2.1 mg/L.

Any other information on results incl. tables

In the control without any test item no immobilisation of the animals was observed. In the test solutions the oxygen concentration did not drop below a value of 60% of the saturation value (» 5 mg O2/L).

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

Under the conditions used for the test, no immobilisation of the daphnids was observed at concentrations representing the water
soluble part (hereafter abbreviated “WAF”, water accommodated fraction) of the test item at concentrations up to 1000 mg/L.

Executive summary:

“308 AQN” was tested for acute toxicity towards daphnia according to OECD-Test Guideline 202 (version dated 13-Apr-2004). In order to investigate the influence of the test item towards the daphnids, the swimming behaviour of the animals was recorded. Under the conditions used for the test, no immobilisation of the daphnids was observed at concentrations representing the water soluble part (hereafter abbreviated “WAF”, water accommodated fraction) of the test item at concentrations up to 1000 mg/L (only screening-test). The main test was performed as a limit test using the WAF from 1000 mg/L “308 AQN”. The following EL-values (effective loading rate) with “308 AQN” are assumed according to the results of this test (definitive test): EL50 >1000 mg/L; NOEL ≥1000 mg/L.