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Diss Factsheets
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EC number: 611-084-9 | CAS number: 54041-17-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- March-August 2014
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with generally accepted scientific standards and described in sufficient detail
- Qualifier:
- according to guideline
- Guideline:
- ISO 10253 (Water quality - Marine Algal Growth Inhibition Test with Skeletonema costatum and Phaeodactylum tricornutum)
- Qualifier:
- according to guideline
- Guideline:
- other: ISO 10253 2006 Water quality - Marine algal growth inhibition test with Skeletonema costatum
- GLP compliance:
- yes (incl. QA statement)
- Analytical monitoring:
- yes
- Remarks:
- HPLC-UV
- Details on sampling:
- - Concentrations: all test concentrations and control sampled
- Sample storage conditions before analysis: below 5 °C - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: FOE-hydroxy was characterised as poorly soluble and test solutions were therefore prepared by Water Accommodated Fractions (WAFs).
- Evidence of undissolved material (e.g. precipitate, surface film, etc): Floating particles - Test organisms (species):
- Skeletonema costatum
- Test type:
- static
- Water media type:
- saltwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 19.1-20.9 °C
- pH:
- 8.06-8.20
- Salinity:
- 36 ‰
- Nominal and measured concentrations:
- Range finding test: Nominal noncentration (mg/L): 1, 10, 100 and 1000, Analytical concentrations at 0 h(mg/L): <0,1, 9,2, 90 and 360, Analytical concentrations at 72h (mg/L): 1, 9, 91 and 930
Definitive test: Nominal concentrations (mg/L): 10, 32, 100, 320 and 1000 , Analytical concentrations at 0h(mg/L): 9, 30, 95, 320 and 1178, Analytical concentrations at 72h(mg/L): 9, 28, 95, 309 and 930 - Details on test conditions:
- TEST SYSTEM
The test was conducted in 100 mL borosilicate glass conical flasks, to which 50 mL of test medium seawater was added. Each treatment was inoculated with cells from the pre-cultures (in exponential growth phase) to give an initial cell density of approximately 10,000 cells per mL.
The initial inoculum was checked microscopically using a haemocytometer. Following inoculation, all flasks were loosely covered with aluminium foil caps and mounted on an orbital shaker at approximately 150 rpm. The illumination consisted of 40W cool white tubes (as specified in the ISO guidelines) which were mounted at a distance of approximately 40 cm directly above the test area. Light intensity values were measured daily during the test. Flasks were assigned positions on the shaker. The controlled temperature room temperature was 20 +-2 °C.
Range finding tests were conducted over 72h to determine the approximate concentrations at which effects were observed. Rangefinding tests employ four concentrations and one replicate per concentration with four controls.
Definitive tests were conducted over 72h at concentrations determined from the results of the Range finding tests. Definitive tests employ five concentrations and three replicates per concentration and six controls. A reference test with 3,5-Dichlorophenol was conducted within the previous three months.
In the definitive test, counts of algal cell numbers were carried out daily by microscope using either haemocytometer or Sedgewick-Rafter chamber, depending upon cell numbers present or by Fluorometer measurements. Three readings were performed on each test vessel
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: The seawater used for this study was supplied by a submersible pump situated on Sutherland’s pier on the west side of Flotta in Scapa Flow, Orkney, Scotland. The site can be referenced from Latitude 58°50'8.02"N, Longitude 3° 8'10.80"W. It is pumped continuously from a depth of two metres below low water spring tide level, before passing up 1.8 kilometres of plastic pipe to a 20,000 litre storage tank. Two smaller pumps move the water to three
settlement tanks situated nine metres above floor level. The seawater temperature varies between 6ºC in the winter and 15ºC in the summer. The salinity is between 36‰ and 38‰.
Culture medium is prepared from natural seawater supplied by pump from Scapa Flow, Orkney. All seawater was UV sterilised and filtered to 0.2 ìm. The filtered treated seawater was then enriched with nutrients and vitamins in accordance with ISO guidelines. The salinity of the enriched natural seawater at 0h was 36‰+- 4‰.
OTHER TEST CONDITIONS
- Sterile test conditions: no
- Adjustment of pH: At pre 0h or 0h, the pH of the culture medium was adjusted if required, by adding 1M HCl, or NaOH to give a pH of 8+-0.2. If, at 0h the pH of the test material stock(s) was outwith the pH range of 8+-0.2 then the pH was returned to within these limits by adjustment with either 1M HCl or NaOH as was appropriate. If the pH requires adjustment, a stirring period was required to ensure the pH remained constant.
- Photoperiod:
- Light intensity and quality: 6100-9250 Lux
- Salinity (for marine algae): 36‰
EFFECT PARAMETERS MEASURED (with observation intervals if applicable):
- Determination of cell concentrations: counts of algal cell numbers were carried out daily by microscope using either haemocytometer or Sedgewick-Rafter chamber, depending upon cell numbers present or by Fluorometer measurements.
TEST CONCENTRATIONS
- Range finding study yes
- Test concentrations: 1, 10, 100 and 1000 mg/L (nominal)
- Results used to determine the conditions for the definitive study: - Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 582 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 95 mg/L
- Nominal / measured:
- meas. (initial)
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Results with reference substance (positive control):
- - Results with reference substance valid? Yes
- EC50: 2.04 mg/L - Validity criteria fulfilled:
- yes
- Conclusions:
- In the definitive test after 72 h FOE-hydroxy exhibited an ErC50 value of 582 mg/L and a NOEC of 95 mg/L(Water Accommodated Fractions (WAFs)) to the marine phytoplankton Skeletonema costatum in the aqueous phase.
- Executive summary:
In the definitive test after 72 h FOE-hydroxy exhibited an ErC50 value of 582 mg/L and a NOEC of 95 mg/L(Water Accommodated Fractions (WAFs)) to the marine phytoplankton Skeletonema costatum in the aqueous phase.
Reference
Description of key information
In the definitive test after 72 h FOE-hydroxy exhibited a ErC50 value of 582 mg/L and a NOEC of 95 mg/L(Water Accommodated Fractions (WAFs)) to the marine phytoplankton Skeletonema costatum in the aqueous phase.
Key value for chemical safety assessment
- EC50 for marine water algae:
- 582 mg/L
- EC10 or NOEC for marine water algae:
- 95 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
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