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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1996
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study without detailed documentation

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 201 (Freshwater Alga and Cyanobacteria, Growth Inhibition Test)
Version / remarks:
Directive 92/69/EEC, C.3
OECD 201 (modified algae growth inhibition test for
dyestuffs).
Deviations:
yes
Remarks:
The test method was modified to quantify not only the algicidal effect of the test item, but also the growth inhibition effect caused by reduced light intensities in the colored test solutions.
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
N,N'-bis{6-chloro-4-[6-(4-vinylsulfonylphenylazo)-2,7-disulfonicacid 5-hydroxy-napht-4-ylamino]-1,3,5-triazin-2-yl}-N-(2-hydroxyethyl)-ethane-1,2-diamine, sodium salt
EC Number:
419-500-9
EC Name:
N,N'-bis{6-chloro-4-[6-(4-vinylsulfonylphenylazo)-2,7-disulfonicacid 5-hydroxy-napht-4-ylamino]-1,3,5-triazin-2-yl}-N-(2-hydroxyethyl)-ethane-1,2-diamine, sodium salt
Cas Number:
171599-85-2
Molecular formula:
C46H38Cl2N14O19S6
IUPAC Name:
tetrasodium 5-[(4-chloro-6-{[2-({4-chloro-6-[(7-{2-[4-(ethenesulfonyl)phenyl]diazen-1-yl}-8-hydroxy-3,6-disulfonatonaphthalen-1-yl)amino]-1,3,5-triazin-2-yl}(2-hydroxyethyl)amino)ethyl]amino}-1,3,5-triazin-2-yl)amino]-3-{2-[4-(ethenesulfonyl)phenyl]diazen-1-yl}-4-hydroxynaphthalene-2,7-disulfonate
Test material form:
solid: particulate/powder

Sampling and analysis

Analytical monitoring:
not specified

Test organisms

Test organisms (species):
Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)

Study design

Test type:
not specified
Water media type:
not specified
Limit test:
no
Total exposure duration:
72 h

Results and discussion

Effect concentrationsopen allclose all
Duration:
72 h
Dose descriptor:
EC50
Remarks:
EbC50 (72h) = 46 mg/l
Effect conc.:
ca. 46 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC50
Remarks:
EbC50 (72h) = 80 mg/l
Effect conc.:
ca. 80 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Duration:
72 h
Dose descriptor:
EC50
Remarks:
EuC50 (72h) > 100 mg/l
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
a toxic effect
of the test item on the algal cells can be excluded up to
the highest test item concentration of 100 mg/L.