Benzamide, N-[[4-[(cyclopropylamino)carbonyl]phenyl]sulfonyl]-2-methoxy-

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

29 Mar - 02 Apr 2004

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)

Qualifier:

according to guideline

Guideline:

other: FIFRA Guideline 72-1

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Sampling method: Water samples were collected from all test vessels and were analyzed at 0 hours (new solutions), and 96 hours (old solutions) to measure actual exposure concentrations. The 10 ml test solution samples for AE 0001789 analysis were collected from mid-depth in each aquarium using a glass pipette.
- Sample storage conditions before analysis: Test solution samples were extracted on the same day as collected.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method:
All 100 mg a.i./L replicates (A, B, and C) were prepared in 38-L glass aquaria that were randomly placed in a 22 ±1°C water bath by filling each aquarium with approximately 25 L of dilution water. Each tank then received approximately 3.08 g (adjusted for 97.4% purity) of the test substance. The solutions were then mixed simultaneously, at room temperature, using electric stirrers. After approximately 30 minutes of mixing, the aquaria were brought to 30 L with dilution water. The solutions were then mixed simultaneously using electric stirrers for approximately 67 hours, no precipitates were visible in any of the aquaria and mixing was stopped. No technical AE 0001789 was added to any of the control tanks.
The mixing technique used was based on solubility work performed prior to study initiation.

- Controls: Yes

Test organisms (species):

Lepomis macrochirus

Details on test organisms:

TEST ORGANISM
- Common name: Bluegill sunfish
- Source: Osage Catfisheries, Inc., Osage Beach, Missouri; fish were received on February 19, 2004
- Length at study initiation: 25.0 to 37.5 mm
- Weight at study initiation: 0.32 to 1.16 g wet weight
- Other: The fish were healthy and no treatments for disease were administered.

ACCLIMATION
- Acclimation period: 48 hours
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: Not feed during this period.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

51 (46-54) mg/L as CaCO3

Test temperature:

22.1 to 22.7 °C

pH:

7.5 to 8.0

Dissolved oxygen:

7.8 to 9.3 mg/L, representing 89 to 106% saturation at 22°C

Nominal and measured concentrations:

Nominal concentrations: 0 (Control) and 100 mg/L
Measured concentrations: <0.61 and 109 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 38 L glass aquaria containing 30 liters of test solution with a water depth of 23.8 cm (aquarium dimensions 49.5 cm(L)x25.4 cm(W)x30.5 cm (H))
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- Biomass loading rate: 0.23 g fish tissue per liter

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Intervals of water quality measurement: Hardness, conductivity, and alkalinity were measured in all test vessels at 0 and 96 hours.
Dissolved oxygen and pH were measured in all test vessels at 0, 48, and 96 hours.

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16-hours light and 8-hours dark
- Light intensity: 58.1 to 67.6 foot candles (mean =691 Lux)

EFFECT PARAMETERS MEASURED: Mortality but sublethal and behavioral effects were also assessed during the course of the study.

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 109 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 109 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: No
- Mortality of control: No
- Any observations: No toxic effects were observed in the dilution water control or the 109 mg a.i./L test level during the exposure period.
- Effect concentrations exceeding solubility of substance in test medium: No undissolved test substance was observed in the test chambers.

Sublethal observations / clinical signs:

Observations:

No treatment related effects were seen at any of the test levels through the course of the study.

Conclusion:

The NOEC, LOEC and LC50 in the 96-Hour exposure of the Bluegill Sunfish (Lepomis macrochirus) to AE 0001789 technical were 109, >109 and >109 mg aiJL, respectively.

 

Table 1: Cumulative Mortality and Behavioral Observations of the Bluegill Sunfish Exposed to AE 0001789 Technical

Mean Measured Conc. (mg a.i./L)	0 Hour		24 Hour		48 Hour		72 Hour		96 Hour
	Dead	Obs	Dead	Obs	Dead	Obs	Dead	Obs	Dead	Obs
Control	0	30 N	0	30 N	0	30 N	0	30 N	0	30 N
109	0	30 N	0	30 N	0	30 N	0	30 N	0	30 N

Obs = Observations (number of individuals observed plus observation).

N = Normal

 

 

Table 2: Measured Test Concentrations During the 96-hour Exposure of the Bluegill Sunfish to AE 0001789 Technical

	Measured AE 0001789 Concentrations (mg a.i./L)
Nominal Conc. (mg a.i./L)	0 Hour (new)	96 Hour (old)	Mean ± Standard Deviation*	Mean Percent of Nominal*
Control A	ND	ND	---	---
Control B	ND	ND
Control C	ND	ND
100 A	113.0	109.1	109±2.5	109
100 B	110.0	106.8
100 C	106.6	107.0

ND = Not detected at or above the validated limit of quantitation (0.61 mg/L).

Conc. = Concentration.

*Calculations for mean, standard deviation, and percent of nominal concentration are based on recoveries from 0 and 96 hours.

Table 3: Validity criteria for OECD 203 (2019)

Criterion from the guideline	Outcome	Validity criterion fulfilled
The mortality in the control(s) should not exceed 10% (or one fish if less than ten are used) at the end of the test.	No mortality in the control(s) was observed at the end of the test.	yes
The dissolved oxygen concentration must have been at least 60% of the air saturation value throughout the test.	The dissolved oxygen concentration was at≥60% of the air saturation value throughout the test (≥7.8 mg/L after 96 hours).	yes
There must be evidence that the concentration of the substance being tested has been satisfactorily maintained, and preferably it should be at least 80% of the nominal concentration throughout the test. If the deviation from the nominal concentration is greater than 20%, results should be based on the measured concentration.	There is evidence that the test concentration was maintained throughout the test (109% of nominal after 96 hours).	yes

 

Validity criteria fulfilled:

yes

Remarks:

See Table 3 in "Any other information on results incl. tables".

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

26 - 30 Mar 2004

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Sampling method: Water samples were collected from all test vessels and were analyzed at 0 hours (new solutions), and 96 hours (old solutions) to measure actual exposure concentrations. The 10 ml test solution samples for AE 0001789 analysis were collected from mid-depth in each aquarium using a glass pipette.
- Sample storage conditions before analysis: Test solution samples were extracted on the same day as collected.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method:
All 100 mg a.i./L replicates (A, B, and C) were prepared in 22-L glass aquaria by filling each aquarium with approximately 15 L of dilution water. Each tank then received approximately 1.85 g (adjusted for 97.4% purity) of the test substance. The solutions were then mixed simultaneously, at room temperature, using electric stirrers. After approximately 30 minutes of mixing, the aquaria were brought to 18 L with dilution water. The solutions were then mixed simultaneously using electric stirrers for approximately 41 hours, no precipitates were visible in any of the aquaria and mixing was stopped. The test solutions were then pumped into aquaria placed in a 12 ± 1°C water bath. The control aquaria (replicates A, B and C) received 18 L of dilution water, while immersed in a 12 ± 1°C test bath, and were stirred with electric mixers for approximately 41 hours. No technical AE 0001789 was added to any of the control tanks. Once mixed, the aquaria containing test solutions were allowed to come to temperature (12 ± 1°C) for approximately 24 hours.
The mixing technique used was based on solubility work performed prior to study initiation. These experiments showed that the material could be solubilized at room temperature (approximately 20°C) after extensive mixing. The solution could then be chilled to 12 ± 1°C with the test material staying in solution. Attempts to prepare 100 mg a.i./L solutions directly in 12 ± 1°C dilution water proved unsuccessful.

- Controls: Yes

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

TEST ORGANISM
- Common name: Rainbow Trout
- Source: Troutlodge, Inc., Sumner, Washington; fish were received on February 12, 2004
- Length at study initiation: 29.0 to 39.0 mm
- Weight at study initiation: 0.34 to 0.83 g wet weight
- Other: The fish were healthy and no treatments for disease were administered.

ACCLIMATION
- Acclimation period: 72 hours
- Acclimation conditions (same as test or not): same as test
- Type and amount of food: Not feed during this period.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

52 (48-54) mg/L as CaCO3

Test temperature:

11.2 to 12.1 °C

pH:

7.4 to 8.0

Dissolved oxygen:

9.0 to 12.4 mg/L, representing 84 to 112% saturation at 12° C

Nominal and measured concentrations:

Nominal concentrations: 0 (Control) and 100 mg/L
Measured concentrations: <0.61 and 101 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 22 L glass aquaria containing 18 liters of test solution with a water depth of 24.3 cm (aquarium dimensions 34.4 cm (L) x 21.6 cm (W) x 30.5 cm (H))
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- Biomass loading rate: 0.30 g fish tissue per liter

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to guideline
- Intervals of water quality measurement: Hardness, conductivity, and alkalinity were measured in all test vessels at 0 and 96 hours.
Dissolved oxygen and pH were measured in all test vessels at 0, 48, and 96 hours.

OTHER TEST CONDITIONS
- Adjustment of pH: No
- Photoperiod: 16-hours light and 8-hours dark
- Light intensity: 56.9 to 75.6 foot candles (mean = 725 lux)

EFFECT PARAMETERS MEASURED: Mortality but sublethal and behavioral effects were also assessed during the course of the study

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 101 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 101 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities: No
- Mortality of control: No
- Any observations: No toxic effects were observed in the dilution water control or the 101 mg a.i./L test level during the exposure period.
- Effect concentrations exceeding solubility of substance in test medium: No undissolved test substance was observed in the test chambers.

Sublethal observations / clinical signs:

Observations:

No treatment related effects were seen at any of the test levels through the course of the study.

Conclusion:

The NOEC, LOEC and LC50 in the 96 -hour exposure of the Rainbow Trout (Oncorhynchus mykiss) to the test substance were 101, ≥101 and >101 mg a.i./L, respectively.

Table 1: Cumulative Mortality and Behavioral Observations of the Rainbow Trout Exposed to AE 0001789 Technical 

Mean Measured Conc. (mg a.i./L)	0 Hour		24 Hour		48 Hour		72 Hour		96 Hour
	Dead	Obs	Dead	Obs	Dead	Obs	Dead	Obs	Dead	Obs
Control	0	30 N	0	30 N	0	30 N	0	30 N	0	30 N
101	0	30 N	0	30 N	0	30 N	0	30 N	0	30 N

Obs = Observations (number of individuals observed plus observation).

N = Normal

 

Table 2: Measured Test Concentrations During the 96-hour Exposure of the Rainbow Trout to AE 0001789 Technical 

	Measured AE 0001789 Concentrations (mg a.i./L)
Nominal Conc. (mg a.i./L)	0 Hour (new)	96 Hour (old)	Mean ± Standard Deviation*	Mean Percent of Nominal*
Control A	ND	ND	---	---
Control B	ND	ND
Control C	ND	ND
100 A	100.4	98.9	101±2.4	101
100 B	105.2	102.7
100 C	99.1	102.5

ND = Not detected at or above the validated limit of quantitation (0.61 mg/L).

Conc. = Concentration.

*Calculations for mean, standard deviation, and percent of nominal concentration are based on recoveries from 0 and 96 hours.

Table 3: Validity criteria for OECD 203 (2019)

Criterion from the guideline	Outcome	Validity criterion fulfilled
The mortality in the control(s) should not exceed 10% (or one fish if less than ten are used) at the end of the test.	No mortality in the control(s) was observed at the end of the test.	yes
The dissolved oxygen concentration must have been at least 60% of the air saturation value throughout the test.	The dissolved oxygen concentration was at≥60% of the air saturation value throughout the test (≥9.0 mg/L after 96 hours).	yes
There must be evidence that the concentration of the substance being tested has been satisfactorily maintained, and preferably it should be at least 80% of the nominal concentration throughout the test. If the deviation from the nominal concentration is greater than 20%, results should be based on the measured concentration.	There is evidence that the test concentration was maintained throughout the test (101% of nominal after 96 hours).	yes

 

Validity criteria fulfilled:

yes

Remarks:

See Table 3 in "Any other information on results incl. tables".

Reference 3

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

24 Jan - 28 Jan 2005

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

adopted 1992

Qualifier:

according to guideline

Guideline:

EPA OPPTS 850.1075 (Freshwater and Saltwater Fish Acute Toxicity Test)

Version / remarks:

Draft, 1996

Qualifier:

according to guideline

Guideline:

other: FIFRA Guideline Number 72-4

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: All test vessels were sampled at test start and test end
- Sampling method: Samples were taken at mid-depth
- Sample storage conditions before analysis: instant analysis

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: The test substance was directly brought into 25 L of water per aquarium and the test solutions were mixed using electric stirrers for approx. 30 min. After the addition of 5 L, the solutions received 25 h of additional mixing with electric stirrers.

Test organisms (species):

Cyprinodon variegatus

Details on test organisms:

TEST ORGANISM
- Common name: Sheepshead minnow
- Lot No.: ABS121504
- Source: Aquatic Biosystems Inc., Fort Collins Co, USA; receiving date 15 Dec 2004
- Length at study initiation (length definition, mean, range and SD): mean: 26.0 mm, range: 20.0 - 31.0 mm, SD 2.9 mm

ACCLIMATION
- Acclimation period: at least 14 days prior to in-life initiation.
- Acclimation conditions (same as test or not): The fish were maintained in culture water at a salinity of 17 to 19 parts per thousand (ppt), pH of 7.7 to 8.0 and temperature of 22 ± 2.0°C in a 16 h daylight fotoperiod
- Type and amount of food: Newly hatched brine shrimp and/or a commercial fish food (Tetramin™, Trout Chow)
- Health during acclimation (any mortality observed): no mortalities, fish were healthy, no treatments for disease were administered

Test type:

static

Water media type:

saltwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

no data

Test temperature:

21.7 - 22.6 °C

pH:

7.6 - 7.9

Dissolved oxygen:

5.4 - 7.4 mg/L
68 - 93% saturation

Salinity:

17 ppt

Nominal and measured concentrations:

Nominal: 0 and 100.0 mg a.i./L
Mean measured (day 0 and 4): < 0.61 and 106.0 mg a.i./L

Details on test conditions:

TEST SYSTEM
- Test vessel: Aquarium
- Material, size, headspace, fill volume: glass, size: 49.5 cm (L) x 25.4 cm (W) x 30.5 cm (H), fill volume: 30 L
- Aeration: Intense aeration of artificial saltwater before use. Gentle aeration of approx. 500 bubbles/min after 48 h for the rest of the study period
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3
- Biomass loading rate: 0.17 g/L

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Water used for estuarine organism studies and culturing in the Bayer CropScience Aquatic Toxicology Laboratory consists of artificial sea salts (HW Marinemix Professional from Hawaiian Marine Imports) mixed with either reverse osmosis water or blended soft water to produce a salinity of approximately 17 ppt. Blended soft water consists of reverse osmosis water blended with spring water to produce soft (40 to 60 mg/L) water. The spring water is collected from a spring box, passed through a multimedia filter, a 5 micron bag filter, granular activated carbon filters, a 1 micron cartridge filter and finally an ultraviolet sterilizer. Reverse osmosis water consists of municipal water that has been dechlorinated with sodium metabisulfite, passed through a multimedia filter, granular activated carbon units, demineralized by conventional softeners, passed through a 5 micron cartridge filter, and treated by double pass reverse osmosis. The automated system constantly monitors the municipal water after dechlorination via a Wallace-Tiernan Micro/2000 flow-through analyzer to ensure that the level of residual chlorine is less than 3 ug/L (ppb). The in-line chlorine analyzer is spot checked for accuracy against a Wallace & Tiernan Amperometric Titrator. Reverse osmosis water and blended water are passed through an ultraviolet sterilizer prior to use in the laboratory and are stored in polypropylene or PVC holding tanks. Both water types are screened weekly for residual chlorine using a Wallace & Tiernan Amperometric Titrator.
- Particulate matter: < 1 mg/L
- Pesticides: Organophosphate pesticides and Atrazine were < 0.02 µg/L, organochlorine pesticides were < 0.05 µg/L, PCBs were < 0.04 µg/L, Chlorinated herbizides in the range of <10 to < 0.2 µg/L
- Chlorine: < 0.003 mg/L
- Ca/Mg ratio: Spring water 13:1, reverse osmosis water approx. < 1000:< 500
- Culture medium different from test medium: no
- Intervals of water quality measurement: Salinity, pH and dissolved oxygen were measured daily in all test vessels. Temperature was controlled hourly.

OTHER TEST CONDITIONS
- Photoperiod: 16 h light/8 h dark
- Light intensity: A 30-minute transition period of lower incandescent lighting was provided at the initiation and termination of each light period to simulate dawn and dusk. The light intensity was adjusted to 50 to 100 foot candles during the light phase (measured mean 606 lux).

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Daily observations were made for mortality and sublethal effects.

TEST CONCENTRATIONS
- Results used to determine the conditions for the definitive study: Based on preliminary data of AE 0001789 no toxicity was expected at 100 mg a.i./L. Therefore this test was run as a limit test with one treatment level in addition to the control group.

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 106 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 106 mg/L

Nominal / measured:

meas. (arithm. mean)

Conc. based on:

act. ingr.

Basis for effect:

mortality (fish)

Details on results:

- Mortality of control: No mortalities or abnormalities in the control vessels
- Mortality in test vessels: No mortalities or abnormal behaviour were observed in any of the test vessels.

Sublethal observations / clinical signs:

Table 1: Cumulative Mortality and Behavioral Observations of the Sheephead Minnow Exposed to AE 0001789 Technical

Mean Measured Conc. (mg a.i./L)	0 Hour		24 Hour		48 Hour		72 Hour		96 Hour
	Dead	Obs	Dead	Obs	Dead	Obs	Dead	Obs	Dead	Obs
Control	0	30 N	0	30 N	0	30 N	0	30 N	0	30 N
106	0	30 N	0	30 N	0	30 N	0	30 N	0	30 N

Dead = Cumulative number dead

Obs = Observations (number of individuals observed plus observation).

N = Normal

 

 

Table 2: Measured Test Concentrations During the 96-hour Exposure of the Sheephead Minnow to AE 0001789 Technical

Nominal Concentration (mg/L)	Rep	Day 0 (new)	Day 4 (old)	Mean	Standard Deviation*	Mean Percent of Nominal*
Control A	A	ND	ND	-	-	-
Control B	B	ND	ND	-	-	-
Control C	C	ND	ND	-	-	-
100 A	A	107.7	106.8	107.3	0.64	-
100 B	B	104.7	106.8	105.8	1.48	-
100 C	C	104.1	106.1	105.1	1.41	-
100 ppm Mean				106.0	-	106%
Lab Spike (102.3)		99.0	93.2	96.1	4.10	94%

ND = Not detected at or above the validated limit of quantitation (0.61 mg/L).

Conc. = Concentration.

Rep = Replicate

*Calculations for mean, standard deviation, and percent of nominal concentration are based on recoveries from Day 0 and Day 4.

Table 3: Validity criteria for OECD 203 (2019)

Criterion from the guideline	Outcome	Validity criterion fulfilled
The mortality in the control(s) should not exceed 10% (or one fish if less than ten are used) at the end of the test.	No mortality in the control(s) was observed at the end of the test.	yes
The dissolved oxygen concentration must have been at least 60% of the air saturation value throughout the test.	The dissolved oxygen concentration was at≥60% of the air saturation value throughout the test (≥6.4 mg/L after 96 hours).	yes
There must be evidence that the concentration of the substance being tested has been satisfactorily maintained, and preferably it should be at least 80% of the nominal concentration throughout the test. If the deviation from the nominal concentration is greater than 20%, results should be based on the measured concentration.	There is evidence that the test concentration was maintained throughout the test (106% of nominal after 96 hours).	yes

 

Validity criteria fulfilled:

yes

Remarks:

See Table 3 in "Any other information on results incl. tables".

Description of key information

LC50 (96 h): >101 mg/L (arith. mean measured, Oncorhynchus mykiss, freshwater, OECD 203)

 

LC50 (96 h): >106 mg/L (arith. mean measured, Cyprinodon variegatus, marine water, OECD 203)

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

> 101 mg/L

Marine water fish

Marine water fish

Dose descriptor:

LC50

Effect concentration:

> 106 mg/L

Additional information

Three GLP short term toxicity studies on freshwater and marine fish were performed (M-251607-02-1, M-240253-01-2 and M-240252-01-1).

Oncorhynchus mykiss were exposed to Cyprosulfamide at a nominal limit concentration of 100 mg/L in a static test for 96 hours, following OECD 203. In the test solutions substance concentrations of 101 mg/L were measured by HPLC. No treatment related effects were observed resulting in a LC50 value of >101 mg/L, based on measured concentrations.

The marine species Cyprinodon variegatus was also exposed to the substance in a limit test for 96 hours, following OECD 203. The nominal concentration of 100 mg/L was verified by HPLC at days 0 and 4 (mean measured: 106.0 mg a.i./L). No treatment related effects were observed resulting in a LC50 value of >106 mg/L, based on measured concentrations.

These results are supported by another OECD 203 study, in which Lepomis macrochirus were exposed to 109 mg/L Cyprosulfamide (mean measured). A LC50 values of >109 mg/L was determined as no treatment related effects were observed.

Concluding from the reported results no acute effects of Cyprosulfamide to fish are expected.