m-toluidinium chloride

Toxicity to microorganisms

Administrative data

Endpoint:

activated sludge respiration inhibition testing

Type of information:

experimental study

Adequacy of study:

key study

Study period:

not available

Reliability:

3 (not reliable)

Justification for type of information:

As m-toluidine hydrochloride dissociates into m-toluidine and hydrochloride the studies concerning the ecotoxicology of m-toludine are considered relevant for the registration according to REACH.

Data source

Reference

Reference Type:

publication

Title:

Screening-level hazard characterization: Monocyclic aromatic amines category

Author:

U.S. Environmental Protection Agency

Year:

2009

Bibliographic source:

U.S. Environmental Protection Agency, September 2009, pp1-32

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test

Deviations:

no

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

m-toluidine

EC Number:

203-583-1

EC Name:

m-toluidine

Cas Number:

108-44-1

Molecular formula:

C7H9N

IUPAC Name:

m-toluidine

Test material form:

not specified

Sampling and analysis

Analytical monitoring:

no

Test organisms

Test organisms (species):

other: other bacteria: activated sludge (from 3L laboratory plant, OECD)

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

3 h

Test conditions

Test temperature:

20.2 to 21.6 °C

pH:

7.4 to 8.6

Nominal and measured concentrations:

100 mg/L, 1,000 mg/L, 10,000 mg/L (nominal)

Details on test conditions:

Aquatic

Reference substance (positive control):

yes

Remarks:

3,5-dichlrophenol

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

3 h

Dose descriptor:

EC10

Effect conc.:

580 mg/L

Basis for effect:

inhibition of total respiration

Effect concentrations 2

Key result

Duration:

3 h

Dose descriptor:

EC50

Effect conc.:

1 572 mg/L

Basis for effect:

inhibition of total respiration

Effect concentrations 3

Duration:

3 h

Dose descriptor:

other: EC5

Effect conc.:

437 mg/L

Basis for effect:

inhibition of total respiration

Results with reference substance (positive control):

3,5-dichlorphenol:
concentration 1.0 mg/L =7.1% inhibition,
concentration 20 mg/L = 78.6% inhibition

Reported statistics and error estimates:

method: probit analysis

Applicant's summary and conclusion

Validity criteria fulfilled:

no

Conclusions:

An EC10 (3h) of 580 mg/L and an EC50(3h) of 1572 mg/L was observed on the respiration of activated sludge.

Executive summary:

As m-toluidine HCl dissociates in aqueous solution into m-toluidine and hydrochloride the studies concerning the ecotoxicology of m-toludine are considered relevant for the registration according to REACH.

The toxicity of m-toluidine towards microorganisms was investigated observing the res-piration of activated sludge. On that basis, an EC10(3h) of 580 mg/L and an EC50(3h) of 1,572 mg/L were determined.

The study has been conducted according to an OECD TG and is not publicly available. As it is not well documented, the study has been rated as Klimisch 3 (not reliable).