bis(2-ethylhexyl) 4,4’-{6-[4-tert-butylcarbamoyl)anilino]-1,3,5-triazine-2,4-diyldiimino}dibenzoate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1997

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

GLP compliance:

yes

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Chemical name of vehicle (organic solvent: Dimethylsulfoxide (DMSO)
- Concentration of vehicle in test medium: 0.1 mL DMSO/L

Test organisms (species):

Daphnia magna

Details on test organisms:

Every week a number of cultures is started with ca. 125 daphnias of the same age (about one day) in ca. 4 litres of dilution water. The cultures are fed daily with ca. 4x10^9 algal cells (chlorella) and ca. 0.13 grams of yeast per 4 litre. The medium is completely replaced at least once a week; at the same time all young born are removed. The cultures are kept at 20+/-1°C under a 16h light and 8h dark regime with transition periods of ca. 30 minutes. After 4 weeks the cultures are discarded.

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

48 h

Hardness:

202 mg/L

Test temperature:

The temperature in one of the control vessels was measured at the beginning and at the end of the test. The temperature in the test vessels was 19.9 at the beginning and 20.6 °C at the end of the test.

pH:

The pH concentration was measured at the beginning and at the end of the test. pH concentration was also measured after 24h in all test media, in which all animals were immobile. The pH varied between 8.0 and 8.3.

Dissolved oxygen:

The oxygen concentration was measured at the beginning and at the end of the test. Oxygen concentration was also measured after 24h in all test media, in which all animals were immobile. The lowest value measured was 8.2 mg/L.

Details on test conditions:

The test was carried out under a 16h light- 8h dark regime with transition periods of ca. 30 minutes in a temperature controlled room.

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 1.88 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

> 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Conclusions:

After 48h a few daphnias were dead at dosed amounts of 3.2, 5.6 and 10 mg/L. This mortality (10% or less) can most likely be attributed to the presence of undissolved material, causing physical hampering of the test organisms. Indications for this phenomenon were obtained in the range finding test, although this could not be visually assessed in the final test. Furthermore, because 10% immobility (in the control) is allowed according to the OECD guideline no. 202, this mortality is not taken into account. It is concluded that the test substance is not acutely toxic to Daphnia magna, with respect to mobility and condition, when exposed at its aqueous solubility level.

Description of key information

Freshwater, 48-h EC50 > 1.88 mg/L (measured concentration), static, Daphnia magna, mobility, OECD TG 202 and EU method C.2, 1997

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Dose descriptor:

EC50

Effect concentration:

> 1.88 mg/L

Additional information

An OECD TG 202 and EU method C.2 followed GLP study is available for this endpoint and used as key study representing freshwater. The 48-hour EC50 for mobility was determined to be > 1.88 mg/L based on measured concentrations (Reliability 1).