bis(2-ethylhexyl) 4,4’-{6-[4-tert-butylcarbamoyl)anilino]-1,3,5-triazine-2,4-diyldiimino}dibenzoate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

October-November 1997

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

yes

Remarks:

0.01 mL/L DMSO

Details on test solutions:

The dilution water was DSWL, prepared from ground water.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Brachydanio rerio
- Source: M.B.Ruysbroek B.V. (Noordvliet 159, Maassluis)
- Length at study initiation: 1.95 +/- 0.11 cm
- Weight at study initiation: 0.06 +/- 0.01 cm
- Amount: 10 fish

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Hardness:

202 mg/L

Test temperature:

24.9-25.6 °C

pH:

7.7-8.3

Dissolved oxygen:

The lowest value was 7.5 mg/L

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 2.48 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 10 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

NOEC

Effect conc.:

>= 2.48 mg/L

Nominal / measured:

meas. (not specified)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Sublethal observations / clinical signs:

There was no mortality in the control medium. No adverse effects observed.

Conclusions:

The test item is not acutely toxic to the zebra fish Brachydanio rerio, with respect to survival and condition, when exposed at its aqueous solubility level.

Description of key information

Freshwater, 96-h LC50 > 2.48 mg/L (measured concentrations), semi-static, Danio rerio, mortality, OECD TG 203 and EU method C.1, 1997

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Dose descriptor:

LC50

Effect concentration:

> 2.48 mg/L

Additional information

An OECD TG 203 and EU method C.1 followed GLP limit study is available for this endpoint and used as the key study representing freshwater (Reliability 1). Ten zebrafish (Danio rerio) per group were exposed to a solvent control (DMSO) and a nominal test substance concentration of 10 mg/L (measured concentration: 2.48 mg/L) in a semi-static system. The test conditions were as follows: temperature 24.9-25.6 °C, pH 7.7-8.3, the lowest dissolved oxygen concentration 7.5 mg/L. The 96-h LC50 was determined to be > 2.48 mg/L, based on measured concentrations.