N-ethyl-N-(3-methylphenyl)propionamide

Short-term toxicity to fish

Administrative data

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

21 July 1996 to 26 July 1996

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1996

Report date:

1996

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Deviations:

no

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

N-ethyl-N-(3-methylphenyl)propionamide

EC Number:

423-640-6

EC Name:

N-ethyl-N-(3-methylphenyl)propionamide

Cas Number:

179911-08-1

Molecular formula:

C12H17NO

IUPAC Name:

N-ethyl-N-(3-methylphenyl)propanamide

Test material form:

liquid

Sampling and analysis

Analytical monitoring:

yes

Details on sampling:

Samples for analysis were taken at 0, 24, 48, 72 and 96 hours old and new for all concentrations.

Test solutions

Vehicle:

no

Details on test solutions:

At -24 hours (21/07/96) 1.51 g of test item (S032) was weighed out and added to 15 litres of carbon filtered tap water (CFTW). This solution was stirred overnight to give a 100 mg/I stock (nominal concentration). This stock was prepared daily and test media prepared by appropriate dilution of the stock as 0, 10, 18, 32, 56 and 100 mg/l

Test organisms

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: Zebra fish
- Strain: Brachydanio rerio
- Length at study initiation (length definition, mean, range and SD): 0.21g
- Weight at study initiation (mean and range, SD): 3.1 cm
- Method of breeding: fed on live and proprietary fish food (Artemis and Tetramin)
- The test fish were isolated 72 hours prior to the start of the test

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Hardness:

111 as mg/l CaCo3

Test temperature:

26.1°C

pH:

7.9

Dissolved oxygen:

7.1

Nominal and measured concentrations:

Nominal: 0, 10, 18, 32, 56 and 100 mg/l
Measured concentrations (geometric mean): 0.0, 5.37, 11.69, 22.39, 43.40 and 100.97 mg/l

Details on test conditions:

TEST SYSTEM
- Test vessel: 6 litre capacity all glace tank
- Type (delete if not applicable): open
- Material, size, headspace, fill volume: 6 litres
- Aeration: Yes
- Renewal rate of test solution (frequency/flow rate): renewal of test medium every 24 hours
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): No replicates
- No. of vessels per control (replicates): none
- No. of vessels per vehicle control (replicates): 1


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : abnormalities in swimming, loss of balance and mortality

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

66 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Conclusions:

The effect concentrations calculated by the binomial method were:

LC50 95% conservative confidence interval (mg/l)
mg/l
24 hours 65 42 - 101
96 hours 66 43 - 101
The lowest concentration causing 100 % mortality at 96 hours was 101 mg/I.
The highest concentration causing 0 % mortality at 96 hours was 43 mg/I.
The 96 hour NOEC (swimming activity) is between 0.0 and 5.4 mg/I.

Executive summary:

The acute toxicity of the test item to the fresh-water fish Brachydanio rerio (zebra fish) was determined according to a standard method conforming to OECD Guideline 203 and EEC Directive 92/69/EEC L383.

 

The test was conducted under static renewal conditions with renewal of test media every 24 hours.

 

Effect concentrations are based on the geometric mean of measured values. The effect concentrations calculated by the binomial method  were:

L h t		LC50	95%conservative confidence interval (mg/I)
		(mg/I)
	24 hours	65	42 - 101
	96 hours	66	43 - 101

 

 

The lowest concentration causing 100% mortality at 96 hours was 101 mg/l.

The highest concentration causing 0 %mortality at 96 hours was 43 mg/l. 

The 96 hour NOEC (swimming activity) is between 0.0  and 5.4 mg/l.