Magnesium dihydrogen di-L-aspartate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

no guideline followed

Principles of method if other than guideline:

- Principle of test:
Determination of oxygen uptake/oxidation of the compound using the Warburg respirometer
- Short description of test conditions:
Exposure of individual chemical compounds at a concentration of 500 mg/l to activated sludge solids at 2,500 mg/l in the Warburg respirometer with oxygen uptake as the measure of oxidation of the compound. The theoretical oxygen demand (TOD) is defined as the concentration of oxygen in mg/L required to oxidize 500 mg/L of substrate completely, i.e., to CO2, water, nitrate, sulfate, and chlorate.

GLP compliance:

no

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Duration of test (contact time):

24 h

Initial conc.:

500 mg/L

Based on:

test mat.

Parameter followed for biodegradation estimation:

O2 consumption

Remarks:

percent from TOD

Reference substance:

other: no

Key result

Parameter:

% degradation (O2 consumption)

Remarks:

percent of TOD

Value:

28.8

Sampling time:

24 h

Validity criteria fulfilled:

not applicable

Interpretation of results:

readily biodegradable

Conclusions:

In the present study of Malaney and Gerhold the biological oxygen demand, i.e. the concentration of oxygen in mg/L required to oxidize 500 mg/L of substrate completely, of activated sludge incubated with DL-aspartic acid was determined. The result reveals that 28.8 % of the compound were degraded within 24 h, therefore DL-aspartic acid is considered to be readily biodegradable.

Description of key information

Based on the published data from Malaney (1969) aspartic acid is readily biodegradable. The additionally contained magnesium in the test item is not considered to have any impact on biodegradability. Furthermore, according to Regulation (EC) No. 1907/2006 (REACh) there is no need for biodegradability testing of magnesium because the susbtance in an inorganic.

Additional information

Based on the published data from Malaney (1969) aspartic acid is readily biodegradable. The additionally contained magnesium in the test item is not considered to have any impact on biodegradability. Furthermore, according to Regulation (EC) No. 1907/2006 (REACh) there is no need for biodegradability testing of magnesium because the susbtance in an inorganic.