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Diss Factsheets
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EC number: 302-766-4 | CAS number: 94134-01-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28/02/2005 - 11/04/2005
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Specific details on test material used for the study:
- T
- Analytical monitoring:
- no
- Details on sampling:
- - Concentrations: 100, 320, 560, 1000, 1800 mg/L
- Sampling method: Population mortality % measured over time. - Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Test material added by water accommodated fractions
- Controls: Negative control (0 mg/L test material) - Test organisms (species):
- other: Scophthalmus maximus
- Details on test organisms:
- TEST ORGANISM
- Common name: Turbot
- Length at study initiation (length definition, mean, range and SD): 38.6 - 42.2 g
- Weight at study initiation (mean and range, SD): 1.671 - 2.272 g
ACCLIMATION
- Acclimation period: 12 days
- Acclimation conditions (same as test or not): No
- Type and amount of food during acclimation: pellet food form
- Feeding frequency during acclimation: at least twice a day
- Health during acclimation (any mortality observed): Mortality observations conducted daily
- Test type:
- semi-static
- Water media type:
- saltwater
- Limit test:
- yes
- Total exposure duration:
- 96 h
- Remarks on exposure duration:
- Measurements taken at 0, 24, 48, 72 and 96 hours
- Test temperature:
- 13.9-15.1 °C
- pH:
- 6.67-8.11 over 96 hours
- Dissolved oxygen:
- 99-103%
- Conductivity:
- 51.4-55.0 mS/cm
- Nominal and measured concentrations:
- Nominal concentrations for test substance were 100, 320, 560, 1000, 1800 mg /L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: soda-lime glass tank
- Material, size, headspace, fill volume: 14 L capacity
- Aeration: gentle aeration
- No. of organisms per vessel: 7
- No. of vessels per concentration (replicates): 1
- No. of vessels per control (replicates): 1
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: Natural seawater from Scapa Flow, Orkney.
- Total organic carbon:
- Particulate matter: Sand filtered to 1 µM
- Alkalinity: pH 7.98
- Intervals of water quality measurement: Daily
OTHER TEST CONDITIONS
- Photoperiod: Constant illumination
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : Mortality in test population
TEST CONCENTRATIONS
- Test concentrations: Nominal concentrations used were 100, 320, 560, 1000 and 1,800 mg/ L-1 - Reference substance (positive control):
- no
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 800 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 800 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 72 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 800 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Key result
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- > 1 800 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- other: LC90
- Effect conc.:
- 1 800 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 1 800 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- - Behavioural abnormalities:
- Mortality of control: Cumulative mortality (0.52%)
- Effect concentrations exceeding solubility of substance in test medium: Effect concentration exceeded maximum concentration tested. - Validity criteria fulfilled:
- yes
- Conclusions:
- The study concludes that the LC50 for 24, 48, 72 and 96 hours was > 1800 mg/L, the LC90 for 96 hours was >1800 mg/L and the NOEC for 96 hours was > 1800 mg/L
- Executive summary:
The study assessed the short-term toxicity of Monoethanolamine Salt of Nitrilotriacetic Acid to marine fish. The study was performed in compliance with GLP and in accordance with the testing guidelines OSPARCOM (1995): Protocol for a fish acute toxicity test. The test organisms used in this study was Scophthalmus maximus and all validity criteria were met. The results from the definitive test looked at 5 different concentrations of the test material (100, 320, 560, 1000 and 1800 mg/L) over 4 different time points (24, 48, 72 and 96 hours) with 1 replicate per concentration (7 organisms per replicate). The definitive test concentrations were derived from the allied Skeletonema toxicity test. Monoethanolamine Salt of Nitrilotriacetic Acid exhibited no effect at 1800 mg/L (direct addition) to the marine fish Scophthalmus maximus in the water phase after 96h.
Reference
Description of key information
The study assessed the short-term toxicity of Monoethanolamine Salt of Nitrilotriacetic Acid to marine fish. The study was performed in compliance with GLP and in accordance with the testing guidelines OSPARCOM (1995): Protocol for a fish acute toxicity test. The test organisms used in this study was Scophthalmus maximus and all validity criteria were met. The results from the definitive test looked at 5 different concentrations of the test material (100, 320, 560, 1000 and 1800 mg/L) over 4 different time points (24, 48, 72 and 96 hours) with 1 replicate per concentration (7 organisms per replicate). The definitive test concentrations were derived from the Skeletonema toxicity test. Monoethanolamine Salt of Nitrilotriacetic Acid exhibited no effect at 1800 mg/L (direct addition) to the marine fish Scophthalmus maximus in the water phase after 96h.
Key value for chemical safety assessment
Marine water fish
Marine water fish
- Effect concentration:
- 1 800 mg/L
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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