Dimethyl (p-methoxybenzylidene)malonate

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2011

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

Daphnia magna

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Reference substance (positive control):

yes

Remarks:

potassium dichromate p. a.

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

1.68 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 95 % confidence limits: 1.38 - 2.05 mg/L

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

1.25 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Duration:

48 h

Dose descriptor:

LOEC

Effect conc.:

2.5 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Validity criteria fulfilled:

yes

Conclusions:

Based on the nominal concentrations ( measured >80%) of the test item, the following results were obtained: EC50 (48 h) = 1.68 mg/L, NOEC (48 h) = 1.25 mg/L and LOEC (48 h) = 2.50 mg/L.

Executive summary:

In the acute immobilization test with Daphnia magna (STRAUS) the effects of the test item Hostavin PR-25 GR were determined according to OECD 202 (2004).Based on the measured concentrations ( measured >80%) of the test item, the 48 -h EC50 for Daphnia magna was estimated to be 1.68 mg/L (95 % confidence limits: 1.38 - 2.05 mg/L). The NOEC after 48 h was determined to be 1.25 mg/L as the highest tested concentration with no biologically significant effect (Immobilization rates of ≤ 10 % are seen to be not significant according to the guideline). The LOEC after 48 h was determined to be 2.50 mg/L as the lowest tested concentration with a biologically significant effect (Immobilization rates of > 10 % are seen to be significant according to the guideline).

Description of key information

48h-EC50 (Daphnia magna, immobilisation) = 1.68 mg/L, 48h-NOEC (Daphnia magna, immobilisation) = 1.25 mg/L; 48h-LOEC (Daphnia magna, immobilisation) = 2.50 mg/L (nominal, OECD 202, 2011)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

1.68 mg/L

Additional information

In a reliable key study, acute immobilisation of Daphnia magna caused by the submission substance was determined according to OECD 202 (2004) and in compliance with GLP (RL1). The study was conducted with five test concentrations (nominal 10, 5, 2.50, 1.25, 0.625 mg/L) under static test conditions during 48 hours. A control was run in parallel. The test concentrations and control were analytically verified by HPLC-DAD analysis. Derived effect concentrations were based on nominal test concentration. Observed effects followed a dose-response relationship with no effect at the lowest test concentration (0.625 mg/L), first occurrence of immobilisation at 1.25 mg/L and complete immobilisation at highest tests concentration (10 mg/L). Derived effect concentrations were: 48h-EC50 = 1.68 mg/L (95 % confidence limits: 1.38 - 2.05 mg/L), 48h-NOEC = 1.25 mg/L and 48h-LOEC = 2.50 mg/L. The study was considered reliable and adequate for the environmental hazard assessment for aquatic organisms.