Dimethyl (p-methoxybenzylidene)malonate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2000

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2000

Report date:

2000

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 423 (Acute Oral toxicity - Acute Toxic Class Method)

GLP compliance:

yes

Test type:

acute toxic class method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Dimethyl (p-methoxybenzylidene)malonate

EC Number:

231-185-8

EC Name:

Dimethyl (p-methoxybenzylidene)malonate

Cas Number:

7443-25-6

Molecular formula:

C13H14O5

IUPAC Name:

1,3-dimethyl 2-[(4-methoxyphenyl)methylidene]propanedioate

Test material form:

solid

Test animals

Species:

rat

Strain:

other: Hanlbm: WIST (SPF)

Sex:

male/female

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

polyethylene glycol

Remarks:

PEG 300

Details on oral exposure:

The animals received a single dose of the test item on a 2000 mg/kg body weight basis by oral gavage following fasting for approximately 17 hours, but with free access to water. Food was provided again 3 hours after dosing.
Dose / kg body weight: 2000 mg
Application volume: 10 ml
Rationale: Oral administration was used as this is one possible route of human exposure during manufacture, handling and use of the test item.

Doses:

2000 mg/kg bw

No. of animals per sex per dose:

3 males or 3 females
Total number of animals: 3 males, 3 females
Age when treated: Males & females: 8 - 10 weeks
Body weight range when treated: Males: 230.4 - 258.2 g, Females: 165.1 - 179.3 g

Control animals:

no

Results and discussion

Effect levels

Key result

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Based on:

test mat.

Remarks on result:

other: after single oral administration to rats of both sexes, observed over a period of 14 days

Mortality:

No death occurred during the study.

Clinical signs:

other: Slight ruffled fur was observed in one female animal (no. 1) from 1 to 5 hours after the administration of the test item. Hunched posture was noted additionally in the same female at the 3- and 5-hour observation. No clinical signs were observed during th

Other findings:

Macroscopic findings: No macroscopic findings were observed at necropsy.

Applicant's summary and conclusion

Interpretation of results:

other: not classified

Conclusions:

The LD50 of the test substance was determined to be LD50(rat) > 2000 mg/kg body weight.