Bis(pentane-2,4-dionato-O,O')zinc

Eye irritation

Administrative data

Endpoint:

eye irritation: in vitro / ex vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 492 (Reconstructed Human Cornea-like Epithelium (RhCE) Test Method for Identifying Chemicals Not Requiring Classification and Labelling for Eye Irritation or Serious Eye Damage)

GLP compliance:

yes (incl. QA statement)

Test material

Test material information

Constituent 1

Reference substance name:

Bis(pentane-2,4-dionato-O,O')zinc

EC Number:

237-860-3

EC Name:

Bis(pentane-2,4-dionato-O,O')zinc

Cas Number:

14024-63-6

Molecular formula:

C10H14O4Zn

IUPAC Name:

zinc bis(4-oxopent-2-en-2-olate)

Test material form:

solid: particulate/powder

Test animals / tissue source

Details on test animals or tissues and environmental conditions:

Triplicate tissues were treated with 30 mg of the test item for 10 minutes. The tissues were dosed at regular timed intervals to allow for the period taken to rinse each insert following exposure and to ensure each tissue received an equal exposure time. The plates were incubated at 37°C, 5% C02 in air during the exposure time.

Test system

Vehicle:

water

Controls:

yes, concurrent positive control

yes, concurrent negative control

Amount / concentration applied:

30 mg of the test

Duration of treatment / exposure:

10 minutes

Duration of post- treatment incubation (in vitro):

The plates were incubated at 37°C, 5% C02 in air during the exposure time.

Number of animals or in vitro replicates:

3 replicates

Results and discussion

In vitro

Results

Irritation parameter:

in vitro irritation score

Run / experiment:

The optical density was measured (quantitative measurement of tissue viability) at 540nm (00540)

Value:

1.4

Vehicle controls validity:

not applicable

Negative controls validity:

valid

Positive controls validity:

valid

Any other information on results incl. tables

Assessment of Eye Irritation Potential - Viability of HCE Tissues

Item	Mean OD540	Relative Mean Viability (%)
Negative Control	0.785	100*
Positive Control	0.036	4.6
Test Item	0.011	1.4

* The mean viability of the negative control tissues is set at 100%.

The relative mean viability of the test item treated tissues after a 10-Minute exposure period was 1.4%.

Applicant's summary and conclusion

Interpretation of results:

Category 2 (irritating to eyes) based on GHS criteria

Conclusions:

Based on the study the test item was considered to be an lrritant.