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Environmental fate & pathways

Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Remarks:
of read across substance
Justification for type of information:
Data for the target chemical is summarized based on the structurally similar read across chemicals
Reason / purpose for cross-reference:
read-across source
Reason / purpose for cross-reference:
read-across source
Qualifier:
according to guideline
Guideline:
other: refer principle below
Principles of method if other than guideline:
WoE derived based on the predicted data for the target chemical and experimental data from structurally and functionally similar read across chemicals
1.Biodegradation of test chemical was analysed by BOD , TOC and by GC parameter for 28 days by taking activated sludge as inoculums.
2.Biodegradability of test chemical was determined by using activated sludge as inoculums in 28 days.
GLP compliance:
not specified
Specific details on test material used for the study:
- Name of test material : Mentha citrata, ext.
- IUPAC name : Mentha citrata, ext.
- Molecular formula : C10H18O (Linalool) or C12H20O2 (Linalyl acetate)
- Molecular weight : 154.251- 196.288 g/mol
- Substance type: Organic
- Physical state: Clear liquid (Colorless to pale yellow)
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
Concentration of activated sludge:30mg/L
Duration of test (contact time):
28 d
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
other: BOD
Parameter followed for biodegradation estimation:
other: TOC
Parameter followed for biodegradation estimation:
other: GC
Parameter followed for biodegradation estimation:
other: ThOD
Details on study design:
1.Standard type
2. TEST SYSTEM
- 2 flasks Treated (basal culture medium + activated sludge + linalyl acetate (100-
102 mg/L));
- 1 flask Positive Control (basal culture medium + activated sludge + Aniline (92
mg/L));
- 1 flask Blank Control (basal culture medium + activated sludge).
Parameter:
other: % degradation BOD
Value:
90
Sampling time:
28 d
Remarks on result:
other: other details not available
Parameter:
% degradation (TOC removal)
Value:
99
Sampling time:
28 d
Remarks on result:
other: other details not available
Parameter:
% degradation (test mat. analysis)
Remarks:
GC
Value:
100
Sampling time:
28 d
Remarks on result:
other: other details not available
Parameter:
other: % degradation by ThOD
Value:
75
Sampling time:
28 d
Remarks on result:
other: other details not available
Validity criteria fulfilled:
not specified
Interpretation of results:
readily biodegradable
Conclusions:
Percent biodegradation of test chemical Mentha citrata, ext. was observed to be 90 % by BOD parameter, 99% by TOC parameter ,100 % by GC parameters, 75 % by ThOD parameter in 28 days. Therefore it is concluded that test chemical Mentha citrata, ext.is readily biodegradable.

Executive summary:

Biodegradation in water of test chemical was predicted for Mentha citrata, ext. (CAS no. 85085-49-0)using data from structurally and functionally similar read across chemicals. The studies are as mentioned below:

The first study was reviwed from authoritative database (2018) in this study the Biodegradation experiment was carried out of test chemical by taking activated sludge as inoculums at 30 mg/L concentration for 28 days. Biodegradation was analyzed by using two parameters namely BOD and GC. The initial concentration of test chemical was 100 mg/L. After 28 days of incubation Percent biodegradation of test chemical was observed to be 90 % by BOD parameter, 99% by TOC parameter and 100 % by GC parameters in 28 days. Therefore it is concluded that test chemical is readily biodegradable.

In another study an experiment was performed to determine biodegradation of test chemical.Activated sludge at concentration 30 mg/L was used as inoculums and it is prepared from soil of river bank, sludge of a municipal treatment plant and sludge of an industrial sewage plant. Incubation was performed for 28 days at 20oC. The oxygen consumption was determined daily with a volumetric respirometer. After IncubationPercent degradation of test chemical was determined to 75 % by considering ThOD as parameter and using Activated sludge as inoculums in 28 days. On the basis of percent degradation value it can be concluded that test chemical is readily biodegradable.

By considering results of both the studies mentioned above it can be concluded that test chemical for Mentha citrata, ext. (CAS no. 85085-49-0) is redaily biodegradable.

Description of key information

Percent biodegradation of test chemical Mentha citrata, ext. was observed to be 90 % by BOD parameter, 99% by TOC parameter ,100 % by GC parameters, 75 % by ThOD parameter in 28 days. Therefore it is concluded that test chemical Mentha citrata, ext.is readily biodegradable.

Key value for chemical safety assessment

Biodegradation in water:
readily biodegradable

Additional information

Biodegradation in water of test chemical was predicted for Mentha citrata, ext. (CAS no. 85085-49-0)using data from structurally and functionally similar read across chemicals. The studies are as mentioned below:

The first study was reviwed from authoritative database (2018) in this study the Biodegradation experiment was carried out of test chemical by taking activated sludge as inoculums at 30 mg/L concentration for 28 days. Biodegradation was analyzed by using two parameters namely BOD and GC. The initial concentration of test chemical was 100 mg/L. After 28 days of incubation Percent biodegradation of test chemical was observed to be 90 % by BOD parameter, 99% by TOC parameter and 100 % by GC parameters in 28 days. Therefore it is concluded that test chemical is readily biodegradable.

In another study an experiment was performed to determine biodegradation of test chemical.Activated sludge at concentration 30 mg/L was used as inoculums and it is prepared from soil of river bank, sludge of a municipal treatment plant and sludge of an industrial sewage plant. Incubation was performed for 28 days at 20oC. The oxygen consumption was determined daily with a volumetric respirometer. After IncubationPercent degradation of test chemical was determined to 75 % by considering ThOD as parameter and using Activated sludge as inoculums in 28 days. On the basis of percent degradation value it can be concluded that test chemical is readily biodegradable.

By considering results of both the studies mentioned above it can be concluded that test chemicalfor Mentha citrata, ext. (CAS no. 85085-49-0) is redaily biodegradable.