Aluminium tributanolate

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Data source

Reference

Reference Type:

publication

Title:

Effects of acute combined inhalation exposure to n-butyl alcohol and n-butyl acetate in experimental animals

Author:

Korsak et al.

Year:

1994

Bibliographic source:

Int J Occup Med Environ Health. 1994;7(3):273-80

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

Deviations:

yes

Remarks:

purpose of the investigations was to asses behavioural effects

GLP compliance:

not specified

Test type:

traditional method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Butan-1-ol

EC Number:

200-751-6

EC Name:

Butan-1-ol

Cas Number:

71-36-3

Molecular formula:

C4H10O

IUPAC Name:

butan-1-ol

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Strain: Imp: DAK
- Weight at study initiation: 250-300 g

ENVIRONMENTAL CONDITIONS: no data

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

whole body

Vehicle:

air

Details on inhalation exposure:

GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: dynamic inhalation chamber (1.3 m3)
- Generation: by heating of liquid substance in washer

TEST ATMOSPHERE
- Brief description of analytical method used: GC with FID
- Samples taken: every 30 min

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

4 h

Concentrations:

ca 3500, 5750 and 7900 ppm (10.8, 17.7 and 24.3 mg/L)

No. of animals per sex per dose:

10 males

Control animals:

not specified

Details on study design:

- Duration of observation period following administration: behavioural tests were performed immediately after the 4 hours exposure period
- Examinations performed:rotarod performance, hot plate performance

Rotarod performance was trained before exposure and only rats that were succesful in at least 10 trials were included in the study. Rotarod performance was measured before exposure and immediately therafter in 10 rats.
Hot plate perfomance was measured as latency to react immediately after exposure in 10 rats.

Statistics:

Probit (EC50), ANOVA and Kruskall-Wallis

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

other: EC50 rotarod

Effect level:

23.2 mg/L air

Based on:

test mat.

95% CL:

>= 19.5 - <= 29.2

Exp. duration:

4 h

Remarks on result:

other: 7559 ppm

Effect levels 2

Sex:

male

Dose descriptor:

other: EC50 hot plate test

Effect level:

18.2 mg/L air

Based on:

test mat.

95% CL:

>= 14.9 - <= 22.3

Exp. duration:

4 h

Remarks on result:

other: 5901 ppm

Effect levels 3

Sex:

male

Dose descriptor:

LC50

Effect level:

> 24.3 mg/L air

Based on:

test mat.

Exp. duration:

4 h

Mortality:

no maortality at any of the concentrations tested

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Conclusions:

The LC 50 of the substance is > 24.3 mg/L. Below this concentration behavioural effects on rotarod performance and hot plate test are observed.