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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
- The test substance was added directly into the study beaker.
Test organisms (species):
Daphnia magna
Details on test organisms:
- Supplier: laboratory breeding
- Feeding: three times a week with green algae (Selenastrum capricornutum)
- During the treatment period the animals were not fed
- Photoperiod: 12/16 h of light/dark
- During the test the animals were kept in the dark

Test type:
static
Water media type:
freshwater
Limit test:
yes
Total exposure duration:
48 h
Hardness:
251 mg/L of CaCO3
Test temperature:
22 °C
pH:
6.5 - 7.7
Dissolved oxygen:
7.0 - 8.0 mg/L
Nominal and measured concentrations:
-Test concentration nominal: 100 mg/L
Details on test conditions:
TEST CONDITIONS
- Test vessel: silylated glass beakers
- No. of organisms per concentration: 20
- Age at the beginning of test < 24 h

TEST MEDIUM / WATER PARAMETERS
- Dilution water according to ISO 6341 - 1982
- sum of calcium and magnesium ions: 25 mmol/L
- Ca/Mg ratio 4:1
- Na/K ratio 10:1

EFFECT PARAMETERS MEASURED
- Observation of immobilization at 24 and 48 hours.
- Parameter checking of dissolved oxygen, pH and temperature at 0 and 48 hours.
Reference substance (positive control):
no
Key result
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
> 100 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
No animals were immobilized in the control group and in the treated group.
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
test procedure in accordance with national standard methods with acceptable restrictions
Remarks:
ISO 14669-1999
Qualifier:
according to guideline
Guideline:
other: ISO 14669-1999: Water quality - Determination of acute lethal toxicity to marine copepods (Copepoda, Crustacea)
GLP compliance:
yes (incl. QA statement)
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
A substance solubility test was carried out by stirring a concentration of 3200 mg/L test substance in saltwater, followed by standstill for 4 hours. By this time it was not dissolved, and further testing was therefore carried out as Water accommodated fractions (WAF).
Water accommodated fractions (WAF) were prepared individually by stirring appropriate amounts of the test substance in growth medium (according to ISO I442:2006). The solutions were stirred with a spin-bar for 22 hours with a speed which formed a vortex one-third of the depth of the fluid content, followed by standstill for about 2 hours. Closed aspirator bottles were used, and samples for testing were drawn off through a drain port near the bottom of the bottle. Test results relate to this water accommodated fraction.


Test organisms (species):
other: Acartia tonsa
Details on test organisms:
TEST ORGANISM
- Source: Acartia tonsa was bought as eggs from Marin Biologisk laboratorium, Kobenhavn University, Helsingor
The eggs were transported by car and airplane to Eurofins at 07.10.2009.
- Age of parental stock: At test start the test organisms were 28 days (after hatching) old.
- Feeding during test: from day 2 on.
- Food type: Rhodomonas baltica

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES:
- The test organisms used in the test were hatched at 13.10.2009 and held at a temperature of 20 ±3 °C.
Test type:
static
Water media type:
saltwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
18.9 - 20.8 °C
pH:
4.6 - 8.3
Dissolved oxygen:
Initial: 7.2 - 7.5 mg/L
after 48 hours: 6.2 - 7.7 mg/L (Only for one test concentration the oxygen content was 0.6 mg/L. Hence for this test vessel the acceptance criteria are not fulfilled)
Salinity:
34.4 per mill
Nominal and measured concentrations:
-Test concentrations nominal: 3, 10, 32, 100, 320, and 1010 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessels: glass containers (28 mL rolled rim vials) each containing 25 mL of test solution.
- Six concentrations of the test substance were tested in a geometric series.
- Four replicates were prepared for each concentration.
- Six replicates were prepared for the blank and for the reference substance.
- The vials were kept under a 16h/8h light/dark photoperiod.

TEST MEDIUM / WATER PARAMETERS
- Natural seawater from an unpolluted site at about 80 m depths in Byfjord was collected from a tap.
- The seawater was filtered through a GF/C filter and briefly heated to about 75 °C prior to use.
- Seawater with addition of nutrient (accordimg to ISO 110253:1998) was used scince an algae test also was conducted.

EFFECT PARAMETERS MEASURED
- Lethality was registered after 24 and 48 hours by visual observation.
- Measurement of oxygen and pH were done at start and after 48 hours.
Reference substance (positive control):
yes
Remarks:
3,5-dichlorphenol
Key result
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
18 mg/L
Nominal / measured:
nominal
Conc. based on:
other: WAF (Water accommodated fraction)
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
10 mg/L
Nominal / measured:
nominal
Conc. based on:
other: WAF
Details on results:
Due to the fact that insufficient data were available for statistical evaluation, the LC50 values were calculated by interpolation.
Reported statistics and error estimates:
Calculation of NOEC is done by single-factor ANOVA followed by Dunnetts procedure for comparing each concentration mean with the control mean.

Description of key information

Toxic effects were observed with marine copepods and no toxicity was observed in a test with freshwater invertebrates.

Key value for chemical safety assessment

Additional information

No data on aquatic toxicity with the salt disodium sebacate are available. Only data on the acid are available:

The 48 h-acute toxicity of sebacic acid to the marine organism Acartia tonsa was studied under static conditions according to ISO 14669-1999 (BASF SE, 2009). The test organisms were exposed to control and test substance at nominal concentrations of 3, 10, 32, 100, 320, and 1010 mg/L for 48 h. Mortality was observed at test termination. The calculated 48 h EC50 was 18 mg/L.

The 48 h-acute toxicity of sebacic acid to the freshwater organism Daphnia magna was studied under static conditions according to OECD Guideline 202 (Ausimont, 1999). The test organisms were exposed to control and test substance at a nominal limit concentration of 100 mg/L for 48 h. No mortality or impaired mobility was observed at test termination. The 48 h EC50 was > 100 mg/L.

However, according to ECHA guidance R7b effects can be extrapolated from the anion or cation, whichever has the most toxic effect, if no data are available on a salt.

If the effect is related to only one of the ions, the classification of the salt should use the effect concentration multiplied by the salt/ion molecular weight ratio.

The molecular weight ratio between salt and ion is 246 : 202 (1.22)

Based on this correction the LC 50 for disodium sebacate was determined to be 22 mg/L in the test with marine copepods and >122mg/L in the test with Daphnia magna.