Reaction mass of diisopropyl hydrogen phosphate and {[hydroxy(propan-2-yloxy)phosphoryl]oxy}(propan-2-yloxy)phosphinic acid and isopropyl dihydrogen phosphate and methyl dihydrogen phosphate and phosphoric acid

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1974

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

no guideline available

Principles of method if other than guideline:

The test was performed according the internal Guideline of the test institute

GLP compliance:

no

Test organisms (species):

Leuciscus idus melanotus

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

11 - 12 °dH

Test temperature:

22 °C +/- 0.5

pH:

7.2 - 7.5

Dissolved oxygen:

> 7.5 mg/L

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

218 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Mortality in the upper concentrations based on the pH value (high acidity)

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity test showed a 48h-LC50 of 218 mg/L.

Executive summary:

The acute toxicity test showed a 48h-LC₅₀ of 218 mg/L.

Reference 2

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1976

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

no guideline available

Principles of method if other than guideline:

The test was performed according the internal Guideline of the test institute

GLP compliance:

no

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

The second species tested was Cyprinus carpio
Salmo gairdneri: 4.5 -12 g, number of fish: 6
Cyprinus carpio: 6.5 - 15 g, number of fish : 6
Depending on the season, the body weight of the fish was so high that in a number of 6 fish per concentration 2 tanks were filled with 3 fish, respectively.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

Salmo gairdneri: 15 +/- 0.5 °C
Cyprinus carpio: 16 +/- 0.5 °C

pH:

Salmo gairdneri: 3.25 - 7.55
Cyprinus carpio: 3.1 - 6. 7

Dissolved oxygen:

Salmo gairdneri: 7.85 - 9. 15 mg/L
Cyprinus carpio: 7.7 - 8.85 mg/L

Nominal and measured concentrations:

Samo gairdneri: 0 - 80 - 100 - 125 - 160 - 200 mg/L
Cyprinus carpio: 0 - 100 - 125 - 160 - 200 mg/L

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

136 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: (salmo gairdneri)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: (salmo gairdneri)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

142 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: (Cyprinus carpio)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

125 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: (Cyprinus carpio)

Details on results:

Mortality in the upper concentrations based on the pH value (high acidity)

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity test showed a 96h-LC50 of 136 mg/L in salmo gairdneri and 142 mg/L in cyprinus carpio.

Executive summary:

The acute toxicity test showed a 96h-LC₅₀of 136 mg/Lin salmo gairdneri and 142 mg/L in cyprinus carpio.

Reference 3

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1974

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

no guideline available

Principles of method if other than guideline:

The test was performed according the internal Guideline of the test institute

GLP compliance:

no

Test organisms (species):

Leuciscus idus melanotus

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

11 - 12 ° dH

Test temperature:

22 °C +/- 0.5

pH:

7.2 - 7.5

Dissolved oxygen:

> 7.5 mg/L

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

282 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Mortality in the upper concentrations based on the pH value (high acidity)

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity test showed a 48h-LC50 of 282 mg/L

Executive summary:

The acute toxicity test showed a 48h-LC₅₀ of 282 mg/L

Reference 4

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

1976

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

no guideline available

Principles of method if other than guideline:

The test was performed according the internal Guideline of the test institute

GLP compliance:

no

Test organisms (species):

Oncorhynchus mykiss (previous name: Salmo gairdneri)

Details on test organisms:

The second species tested was Cyprinus carpio
Salmo gairdneri: 2.6 - 12.3 g, number of fish: 6
Cyprinus carpio: 5.1 - 14.9 g, number of fish : 6
Depending on the season, the body weight of the fish was so high that in a number of 6 fish per concentration 2 tanks were filled with 3 fish, respectively.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test temperature:

Salmo gairdneri: 15 +/- 0.5 °C
Cyprinus carpio: 16 +/- 0.5 °C

pH:

Salmo gairdneri: 3.65 - 7.7
Cyprinus carpio: 3.1 - 6. 9

Dissolved oxygen:

Salmo gairdneri: 7.8 - 9. 35 mg/L
Cyprinus carpio: 8.2 - 9.45 mg/L

Nominal and measured concentrations:

Samo gairdneri: 0 - 50 - 80 - 125 - 160 - 200 mg/L
Cyprinus carpio: 0 - 100 - 125 - 160 - 200 mg/L

Reference substance (positive control):

no

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

130 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: (salmo gairdneri)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

80 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: (salmo gairdneri)

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

142 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: (Cyprinus carpio)

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

125 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: (Cyprinus carpio)

Details on results:

Mortality in the upper concentrations based on the pH value (high acidity)

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity test showed a 96h-LC50 of 130 mg/L in salmo gairdneri and 142 mg/L in cyprinus carpio.

Executive summary:

The acute toxicity test showed a 96h-LC₅₀ of 130 mg/Lin salmo gairdneri and 142 mg/L in cyprinus carpio.

Reference 5

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Justification for type of information:

Please refer to chapter 13

Reason / purpose for cross-reference:

read-across source

Duration:

48 h

Dose descriptor:

LC0

Effect conc.:

282 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Reference 6

Endpoint:

short-term toxicity to fish

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

weight of evidence

Justification for type of information:

Please refer to chapter 13

Reason / purpose for cross-reference:

read-across source

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

130 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: salmo gairdneri

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

90 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: salmo gairdneri

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

142 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Cyprinus carpio

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

125 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Remarks on result:

other: Cyprinus carpio

Validity criteria fulfilled:

yes

Conclusions:

The acute toxicity test showed a 96h-LC50 of 130 mg/L in salmo gairdneri and 142 mg/L in cyprinus carpio.

Executive summary:

The acute toxicity test showed a 96h-LC50 of 130 mg/L in salmo gairdneri and 142 mg/L in cyprinus carpio.

Description of key information

Four short-term toxicity to fish tests with three different species are available.

The tests with salmo gairdneri shows a 96 h-LC₅₀between 130 and 136 mg/L. The 96 h- LC₅₀on Cyprinus carpio was found to be 142 mg/L. The 48 h LC₀on Leuciscus idus was found to be between 218 and 282 mg/L.

All results are above 100 mg/L and therefore it can be concluded that the registered substance is not toxic to fish.

Additional information

Four short-term toxicity to fish tests with three different species are available. The tests with salmo gairdneri shows a 96 h-LC₅₀between 130 and 136 mg/L. The 96 h- LC₅₀on Cyprinus carpio was found to be 142 mg/L. The 48 h LC₀on Leuciscus idus was found to be between 218 and 282 mg/L.

All results are above 100 mg/L and therefore it can be concluded that the registered substance is not toxic to fish.