Registration Dossier
Registration Dossier
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EC number: 279-131-2 | CAS number: 79295-99-1
Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
Description of key information
ErL50 > 5 mg/l
Key value for chemical safety assessment
Additional information
No studies on the "toxicity to aquatic algae and cyanobacteria" are available for the substance in itself. Nevertheless studies have been conducted with an analogue molecule (Similar Substance 01). Further information are reported in the Read Across justification attached to section 13.
The toxicity of the test substanceto the unicellular freshwater green alga Pseudokirchneriella subcapitata was determined according to the principles of OECD 201.The aim of the study was the determination of NOEL, LOEL, EL10, EL20- and EL50-values of growth rate and yield over a period of 72 hours.
The study was conducted under static conditions with an initial cell density of 6839 cells/mL. A saturated solution with a nominal loading of 5.00 mg test item/L was prepared with dilution water 24 ± 1 hour prior to the start of the exposure. An appropriate amount of the test item was weighed out. The saturated solution was stirred for 24 ± 1hour (1100 rpm) with a magnetic stirrer. Undissolved particles were removed by membrane filtration. The following filtrate, i.e. the saturated solution was used in the test. The solution was checked via laser beam (Tyndall effect), which was negative.
The saturated solution and four dilution levels out of the saturated solution were tested in the definitive test in a geometrical series with a dilution factor of 2: 6.25 - 12.5 - 25.0 - 50.0 - 100 % of the saturated solution. Three replicates were tested for each test item concentration and six replicates for the control.The test media were clear throughout the test period.The environmental conditionswere within the acceptable limits.
The measured concentration of the test item was even < LOD at the start of the exposure (0 hours), therefore no analysis at the end of the exposure was necessary.
All effect values given are based on the nominal test item loadings.
NOEL, LOEL, ELx-values(0 - 72 hours)
based on the nominal test item loadings
|
Growth Rate Inhibition |
NOEL |
5.00 |
LOEL |
> 5.00 |
ErL10 |
> 5.00 |
ErL20 |
> 5.00 |
ErL50 |
> 5.00 |
|
Yield Inhibition nominal test item loadings mg/L] |
NOEL |
5.00 |
LOEL |
> 5.00 |
EyL10 |
> 5.00 |
EyL20 |
> 5.00 |
EyL50 |
> 5.00 |
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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