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EC number: 279-131-2 | CAS number: 79295-99-1
Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
Description of key information
EL50 (48h) > 5 mg/l
Key value for chemical safety assessment
Additional information
No studies on the "short-term toxicity to aquatic invertebrates" are available for the substance in itself.
Nevertheless studies have been conducted with a analogue molecules (Similar Substance 02 and 04). Further information are reported in the Read Across justification attached to section 13.
In the key study the substance (Similar Substance 02) was tested on Daphnia magna (STRAUS), according to OECD guideline 202. The effects of a saturated solution with a nominal loading of 5.00 mg/L of the test item were determined under static conditions over a period of 48 hours. The saturated solution was visually clear during the exposure period. Twenty daphnids, divided into 4 replicates with 5 daphnids each, were exposed to the saturated solution and the control.
The concentration of the test item was analytically verified via HPLC-DAD in fresh media at the start of the exposure (0 hours) in the saturated solution and in the control.
The measured concentration in the saturated solution was < LOD (LOD = 0.05 mg/L with a S/N ratio of 3) at the start of the exposure (0 hours).Therefore, analytical monitoring at the end of the exposure was waived and the test item concentration was considered to be below the LOD in the 48-hours old media, too.
According to OECD Guidance Document No 23 (2000), if an effect cannot be detected in a test with a saturated solution, the result has to be reported as no toxic effects at saturation. Exposures are expressed in terms of the original concentration of the test item in water at the start of the mixing period (loading rate).
The validity criteria of the test guideline were fulfilled.
EL10 / 50 / 100-Values(based on the nominal loading of the test item)
Effect values |
Test duration [hours] |
Disperse Violet93.1 Nominal loading level of the test item [mg/L] |
EL10 / 50 / 100 |
24 |
> 5.00 |
48 |
> 5.00 |
In the supporting study the substance (Similar Substance 04) was tested for its acute toxicity to Daphnia magna, according to the OECD guideline 202, at 0.9, 1.9, 4.3, 9.4, 21, 45, 100 mg/l. The EC50 calculated in this test was greater than 100 mg/l.
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