Hexane, 1,6-diisocyanato-, homopolymer, 2-hydroxyethyl acrylate- and propylene glycol monoacrylate-blocked

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

From November 23, 2016 to November 25, 2016

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Remarks:

DOC determination using a TOC analyser

Details on sampling:

At the start and at the end of the test, the content of the test substance in the test solutions was determined using DOC determination.

Vehicle:

no

Details on test solutions:

Water-accommodated fractions (WAF) were prepared for the test. This was done by weighing the nominal loads of the test substance, adding the corresponding amount of dilution water and shaking vigorously for 24 h. The resulting solution was filtered through 0.45 μm nylon filters.

Test organisms (species):

Daphnia magna

Details on test organisms:

Specification:
Species Daphnia magna;
Variety: STRAUS;
Strain: Berlin;
Sex: female;
Age: between 0 and 24 h;
Origin: Umweltbundesamt Berlin;
In-house breeding since September, 2007;
Selection of the test system was made following the proposal of the guidelines.
Animal Husbandry: Daphnia magna is bred at LAUS GmbH throughout the year. The animals are kept for the use in toxicity tests. They multiply by parthenogenesis, thus being genetically identical. The husbandry is performed similar to the method described in the OECD guidelinesand follow internal SOPs.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

Resulting hardness in mmol/L: 2.502 mmol/L
Resulting hardness in mg CaCO3/L: 250

Test temperature:

19.2 – 21.0°C

pH:

7.8 - 8.8

Dissolved oxygen:

not specified

Salinity:

not specified

Conductivity:

not specified

Nominal and measured concentrations:

Nominal: 0, 4.6, 10, 22, 46 and 100 mg/L
Measured in t = 0 h and t = 48 h
geometric mean: 0, 1.9, 4.3, 8.4, 17.1 and 33.8 (mg/L)
Nominal and measured concentrations of the test substance in blank controls: 0

Reference substance (positive control):

yes

Remarks:

Potassium dichromate K2Cr2O7

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 34 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 34 mg/L

Nominal / measured:

meas. (geom. mean)

Conc. based on:

test mat.

Basis for effect:

mobility

Key result

Duration:

48 h

Dose descriptor:

NOEC

Effect conc.:

>= 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Results with reference substance (positive control):

Potassium dichromate (K2Cr2O7; CAS No. 7778-50-9) was used as positive control in a reference study to assure that the test conditions are reliable.

Validity criteria fulfilled:

yes

Conclusions:

Under the study conditions, the measured 48 h EC50 of the substance to Daphnia magna was determined to be >34 mg/L (equivalent to a nominal level of >100 mg/L).

Executive summary:

A study was conducted to determine the toxicity of the substance to Daphnia magna according to OECD Guideline 202 and EU Method C.2, in compliance with GLP. The study was performed using 5 concentrations of the test substance ranging from 4.6 to 100 mg/L (nominal). Test solutions were prepared by the water-accommodated fractions (WAF) method. At the start and at the end of the test, the content of the test substance in the test solutions was estimated by calculation of the organic carbon content and measurement of dissolved organic carbon (DOC). For each test concentration, 20 daphnids were exposed to the test substance for 48 h in a static test system. After 24 and 48 h, the immobilised Daphnia were counted. The test concentrations showed no toxicity at any of the tested concentrations. Under the study conditions, the measured 48 h EC50 of the substance to Daphnia magna was determined to be >34 mg/L (equivalent to a nominal level of >100 mg/L). (Muckle, 2017).

Description of key information

.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

34 mg/L

Additional information

A study was conducted to determine the toxicity of the substance to Daphnia magna according to OECD Guideline 202 and EU Method C.2, in compliance with GLP. The study was performed using 5 concentrations of the test substance ranging from 4.6 to 100 mg/L (nominal). Test solutions were prepared by the water-accommodated fractions (WAF) method. At the start and at the end of the test, the content of the test substance in the test solutions was estimated by calculation of the organic carbon content and measurement of dissolved organic carbon (DOC). For each test concentration, 20 daphnids were exposed to the test substance for 48 h in a static test system. After 24 and 48 h, the immobilised Daphnia were counted. The test substance showed no toxicity at any of the tested concentrations. Under the study conditions, the measured 48 h EC50 of the substance to Daphnia magna was determined to be >34 mg/L (equivalent to a nominal level of >100 mg/L). (Muckle, 2017).