Registration Dossier
Registration Dossier
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EC number: 275-279-7 | CAS number: 71230-67-6
Acute Toxicity: oral
Administrative data
- Endpoint:
- acute toxicity: oral
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- secondary literature
- Justification for type of information:
- Data is from secondary source
Data source
Reference
- Reference Type:
- secondary source
- Title:
- OECD SIDS Initial Assessment Report (CAS no. 4404-43-7).
- Author:
- OECD SIDS – SIAM
- Year:
- 2 005
- Bibliographic source:
- OECD SIDS Initial Assessment Report, SIAM 20, April 2005.
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: as below
- Principles of method if other than guideline:
- Acute oral toxicity study of Benzenesulfonic acid,2-2’-(1,2-ethenediyl)bis(5-((4-(bis(2- hydroxyethyl)amino)-6-(phenylamino)-1,3,5-triazin-2-yl)amino)-, free acid in rat
- GLP compliance:
- not specified
- Test type:
- other: not specified
- Limit test:
- no
Test material
- Reference substance name:
- 4,4'-bis[4-[bis(2-hydroxyethyl)amino]-6-anilino-1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulphonic acid
- EC Number:
- 224-548-7
- EC Name:
- 4,4'-bis[4-[bis(2-hydroxyethyl)amino]-6-anilino-1,3,5-triazin-2-yl]amino]stilbene-2,2'-disulphonic acid
- Cas Number:
- 4404-43-7
- Molecular formula:
- C40H44N12O10S2
- IUPAC Name:
- 2,2'-ethene-1,2-diylbis[5-({4-anilino-6-[bis(2-hydroxyethyl)amino]-1,3,5-triazin-2-yl}amino)benzenesulfonic acid]
- Test material form:
- solid
- Details on test material:
- - Name of test material (as cited in study report): Benzenesulfonic acid, 2,2'-(1,2-ethenediyl)bis[5-[[4-[bis(2-hydroxyethyl) amino]-6-(phenylamino)-1,3,5-triazin-2-yl]amino]-
- Molecular formula (if other than submission substance): C40H44N12O10S2
- Molecular weight (if other than submission substance): 916.994 g/mole
- Smiles notation (if other than submission substance): c1(nc(Nc2cc(c(\C=C\c3c(cc(Nc4nc(N(CCO)CCO) nc(n4)Nc4ccccc4)cc3)S(O)(=O)=O)cc2)S(O) (=O)=O)nc(n1)Nc1ccccc1)N(CCO)CCO
- InChl (if other than submission substance): 1S/C40H44N12O10S2/c53-21-17-51(18-22-54)39-47-35(41-29-7-3-1-4-8-29)45-37(49-39)43-31-15-13-27(33(25-31)63(57,58)59)11-12-28-14-16-32(26-34(28)64(60,61)62)44-38-46-36(42-30-9-5-2-6-10-30)48-40(50-38)52(19-23-55)20-24-56/h1-16,25-26,53-56H,17-24H2,(H,57,58,59)(H,60,61,62) (H2,41,43,45,47,49)(H2,42,44,46,48,50)/b12-11+
- Structural formula attached as image file (if other than submission substance): see Fig.
- Substance type: organic
- Physical state: solid
- Analytical purity: 89%
- Impurities (identity and concentrations):11%
Constituent 1
- Specific details on test material used for the study:
- - Name of test material (as cited in study report): Benzenesulfonic acid, 2,2'-(1,2-ethenediyl)bis[5-[[4-[bis(2-hydroxyethyl) amino]-6-(phenylamino)-1,3,5-triazin-2-yl]amino]-
- Molecular formula (if other than submission substance): C40H44N12O10S2
- Molecular weight (if other than submission substance): 916.994 g/mole
- Smiles notation (if other than submission substance): c1(nc(Nc2cc(c(\C=C\c3c(cc(Nc4nc(N(CCO)CCO) nc(n4)Nc4ccccc4)cc3)S(O)(=O)=O)cc2)S(O) (=O)=O)nc(n1)Nc1ccccc1)N(CCO)CCO
- InChl (if other than submission substance): 1S/C40H44N12O10S2/c53-21-17-51(18-22-54)39-47-35(41-29-7-3-1-4-8-29)45-37(49-39)43-31-15-13-27(33(25-31)63(57,58)59)11-12-28-14-16-32(26-34(28)64(60,61)62)44-38-46-36(42-30-9-5-2-6-10-30)48-40(50-38)52(19-23-55)20-24-56/h1-16,25-26,53-56H,17-24H2,(H,57,58,59)(H,60,61,62) (H2,41,43,45,47,49)(H2,42,44,46,48,50)/b12-11+
- Structural formula attached as image file (if other than submission substance): see Fig.
- Substance type: organic
- Physical state: solid
Test animals
- Species:
- rat
- Strain:
- Wistar
- Remarks:
- II
- Sex:
- female
- Details on test animals or test system and environmental conditions:
- - Weight at study initiation: 150 - 200 g bw
Administration / exposure
- Route of administration:
- oral: gavage
- Vehicle:
- water
- Details on oral exposure:
- VEHICLE
- Concentration in vehicle: 15,000 mg/kg
bw
- Amount of vehicle (if gavage): No data available
- Justification for choice of vehicle: Water
- Lot/batch no. (if required):
- Purity:
MAXIMUM DOSE VOLUME APPLIED:
DOSAGE PREPARATION (if unusual): 15000 mg/kg bw test substance was dissolved in water (3% solution). - Doses:
- 15000 mg/kg bw
- No. of animals per sex per dose:
- 10
- Control animals:
- not specified
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: No data available
- Necropsy of survivors performed: yes/no
- Other examinations performed: Mortality and clinical signs were observed. - Statistics:
- Probir analysis (Fink & Hund, Arzenimittelforschung 15, 624, 1965) was used.
Results and discussion
- Preliminary study:
- not specified
Effect levels
- Sex:
- female
- Dose descriptor:
- LD50
- Effect level:
- > 15 000 mg/kg bw
- Based on:
- test mat.
- Remarks on result:
- other: No mortality and clinical sign observed
- Mortality:
- No mortality was observed in treated female rat at 15000 mg/kg bw
- Clinical signs:
- other: No clinical sign were observed in treated female rat at 15000 mg/kg bw
- Gross pathology:
- not specified
- Other findings:
- not specified
Applicant's summary and conclusion
- Interpretation of results:
- Category 5 based on GHS criteria
- Conclusions:
- LD50 was considered to be >15000 mg/kg bw when 10 Wistar II female rat was treated with Benzenesulfonic acid,2-2’-(1,2-ethenediyl)bis(5-((4-(bis(2- hydroxyethyl)amino)-6-(phenylamino)-1,3,5-triazin-2-yl)amino)-, free acid orally by gavage.
- Executive summary:
In a acute oral toxicity study, 10 Wistar II female rat was treated with Benzenesulfonic acid,2-2’-(1,2-ethenediyl)bis(5-((4-(bis(2- hydroxyethyl)amino)-6-(phenylamino)-1,3,5-triazin-2-yl)amino)-, free acid in the concentration of 15000 mg/kg bw orally by gavage in water (3 % solution). No mortality and clinical signs were observed in treated female rat at 15000 mg/kg bw.Therefore, LD50 was considered to be >15000 mg/kg bw when 10 Wistar II female rat was treated withBenzenesulfonic acid,2-2’-(1,2-ethenediyl)bis(5-((4-(bis(2- hydroxyethyl)amino)-6-(phenylamino)-1,3,5-triazin-2-yl)amino)-, free acid orally by gavage.
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