Dimethyl vinylphosphonate

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Study period:

3 May 1990 and 18 May 1990

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 203 (Fish, Acute Toxicity Test)

Version / remarks:

04 April 1984

Deviations:

no

Qualifier:

according to guideline

Guideline:

EU Method C.1 (Acute Toxicity for Fish)

Version / remarks:

25 April 1984

Deviations:

no

GLP compliance:

yes (incl. QA statement)

Analytical monitoring:

yes

Details on sampling:

- Concentrations: 500 mg/L
- Sampling method: The test substance was diluted in dilution water, homogenized in an ultrasonic bath and with Ultra-Turrax and introduced into the test vessel (10 L fill volume) with stirring. A volume of 100 mL of the sample was taken from the center of the test vessel after 48 and 96 hours.

Vehicle:

no

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was diluted in dilution water, homogenized in an ultrasonic bath and with Ultra-Turrax and introduced into the test vessel (10 L fill volume) with stirring.

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

TEST ORGANISM
- Common name: zebra fish
- Strain: Brachydanio rerio
- Source: West Aquarium, 3422 Bad Lauterberg
- Length at study initiation: 2.7 - 3.4 cm

ACCLIMATION
- Acclimation period: at least 14 days
- Acclimation conditions (same as test or not): Yes
- Type and amount of food during acclimation: Tetra Min, Tetra Werke, Melle; libitum
- Feeding frequency during acclimation: twice daily


FEEDING DURING TEST: No

Test type:

static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

96 h

Test temperature:

22.4 - 22.7 °C (test group)
21.8 - 22.5 °C (control group)

pH:

7.6 - 8.0 (test group)
7.9 - 8.2 (control group)

Dissolved oxygen:

7.2 - 9.3 O2 mg/L (test group)
7.5 - 9.2 O2 mg/L (control group)

Salinity:

not applicable

Nominal and measured concentrations:

nominal concentration: 500 mg/L
measured concentration: 482 mg/L (0 h), 493 mg/L (48 h), 525 mg/L (96 h)

Details on test conditions:

TEST SYSTEM
- Material, size, fill volume: glass, 30 x 22 x 24 cm (length x width x height), 10 L
- Aeration: none
- No. of organisms per vessel: 10
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10

TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: according to ISO/DIS 7346/1
- Culture medium different from test medium: no
- Intervals of water quality measurement: 0 h, 2-4 h, 24 h, 48 h, 72 h and 96 h

OTHER TEST CONDITIONS
- Adjustment of pH: none
- Photoperiod: 12 hours light
- Light intensity: 700 lux

EFFECT PARAMETERS MEASURED:
- Mortality, visible abnormalities and behaviour (observation were made after 2-4, 24, 48, 72 and 96 hours)

Reference substance (positive control):

no

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

48 h

Dose descriptor:

LC50

Effect conc.:

> 500 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

- Behavioural abnormalities:
- Other biological observations: changed swimming behaviour, changed appearance and behaviour, impaired breathing
- Mortality of control: no
- Other adverse effects control: no

Validity criteria fulfilled:

yes

Conclusions:

The test item was examined for acute toxicity to freshwater fish in a 96-h static test according to OECD Guideline 203 and EU method C.1. The test item caused no mortality up to a nominal concentration of 500 mg/L. Thus, the 96-h LC50 was determined to be greater than 500 mg/L.

Executive summary:

The acute toxicity of the test item to fish (zebrafish) was determined according to the OECD Guideline 203 and EU method C.1.

A static test was performed as a limit test with a concentration of 500 mg/L. Ten test organisms were exposed to the test concentration and the control, respectively. Mortality, visible abnormalities and changes in behaviour were determined after 2-4, 24, 48, 72 and 96 hours. The water quality parameters pH-value, temperature and oxygen saturation were measured after 0, 2-4, 24, 48, 72 and 96 h.

The measured concentration of the test item was greater than 80% of the nominal value. 

Test organisms exposed to 500 mg/L of the test item showed partly changes in behaviour and appearance as well as an impaired breathing. The test item caused no mortality up to a nominal concentration of 500 mg/L. Thus, the 96-h LC50 was determined to be greater than 500 mg/L.

Description of key information

The test item was examined for acute toxicity to freshwater fish in a 96-h static test according to OECD Guideline 203 and EU method C.1. The test item caused no mortality up to a nominal concentration of 500 mg/L. Thus, the 96-h LC50 was determined to be greater than 500 mg/L.

Additional information

Acute Toxicity to Fish

The acute toxicity of the test item to fish (zebrafish) was determined according to the OECD Guideline 203 and EU method C.1.

A static test was performed as a limit test with a concentration of 500 mg/L. Ten test organisms were exposed to the test concentration and the control, respectively. Mortality, visible abnormalities and changes in behaviour were determined after 2-4, 24, 48, 72 and 96 hours. The water quality parameters pH-value, temperature and oxygen saturation were measured after 0, 2-4, 24, 48, 72 and 96 h.

The measured concentration of the test item was greater than 80% of the nominal value. 

Test organisms exposed to 500 mg/L of the test item showed partly changes in behaviour and appearance as well as an impaired breathing. The test item caused no mortality up to a nominal concentration of 500 mg/L. Thus, the 96-h LC50 was determined to be greater than 500 mg/L.