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Environmental fate & pathways

Biodegradation in water: screening tests

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Endpoint:
biodegradation in water: screening test, other
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
1989
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
documentation insufficient for assessment
Qualifier:
no guideline followed
Principles of method if other than guideline:
A static test was conducted to determine the biodegradability of the test item. The biodegradation was determined after 3 hours, 5 days, 10 days and 28 days.
GLP compliance:
no
Oxygen conditions:
not specified
Inoculum or test system:
not specified
Duration of test (contact time):
28 d
Parameter followed for biodegradation estimation:
DOC removal
Parameter followed for biodegradation estimation:
other: COD removal
Parameter followed for biodegradation estimation:
other: BOD
Key result
Parameter:
not specified
Value:
0
Sampling time:
28 d
Details on results:
The EC50 value of the toxicity control was greater than 5000 mg/L.
Validity criteria fulfilled:
not specified
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
The degree of biodegradation was 0% after an exposure of 28 days. Thus, the test item is not considered to be biodegradable under the test conditions chosen.
Executive summary:

The biodegradation of the test item was determined in a static test after 3 hours, 3, 5, 10 and 28 days. The degree of biodegradation was 0% after an exposure of 28 days. The EC50 value of the toxicity control was determined to be greater than 500 mg/L. Thus, the test item is not considered to be biodegrdable under the test conditions chosen.

Endpoint:
biodegradation in water: inherent biodegradability
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
March 1990 - January 1991
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
OECD Guideline 302 B (Inherent biodegradability: Zahn-Wellens/EMPA Test)
Version / remarks:
not specified
Deviations:
yes
Remarks:
No reference control was testesd. A bacteria toxicity control is not specified.
GLP compliance:
yes
Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic (adaptation not specified)
Details on inoculum:
- Source of inoculum/activated sludge: HOECHST/Kelsterbach 1:1
- Concentration of sludge: 6 g/L (wet) corresonding to 564 mg/L dry weight)
Duration of test (contact time):
3 h
Initial conc.:
617 mg/L
Based on:
COD
Parameter followed for biodegradation estimation:
other: COD removal
Details on study design:
SAMPLING
- Sampling frequency: after 3 hours, 1 d, 3, 6, 8, 10, 13, 16, 20, 22, 24, 27 and 28 days
Reference substance:
not specified
Key result
Parameter:
other: % degradation (COD removal)
Value:
6
Sampling time:
28 d
Details on results:
The adsorption after 3 hours was less than 10%. The test was terminated after 28 days.
Validity criteria fulfilled:
no
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
The biodegradation of the test substance was determined to be less than 6% after 28 days. Thus, the test item is not considered to be inherently biodegradable under the test conditions chosen.
Executive summary:

The inherent biodegradability of the test item was investigated in a Zahn-Wellens-Test according to OECD Guideline 302B. Test test substance was tested at a nominal concentration of 617 mg/L (COD). The test was terminated after 28 days. The adsorption after 3 hours was less than 10%. The biodegradation of the test substance was determined to be less than 6% after 28 days. Thus, the test item is not considered to be inherently biodegradable under the test conditions chosen.

Description of key information

The test item was not inherently biodegradable under the test conditions chosen (% degradation less than 10%) in an Zahn-Wellens-Test. Furthermore, the degree of biodegradation was 0% in a static test.

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

A WoE approach was conducted to assess the biodegradation of the test item.

The inherent biodegradability of the test item was investigated in a Zahn-Wellens-Test according to OECD Guideline 302B. Test test substance was tested at a nominal concentration of 617 mg/L (COD). The test was terminated after 28 days. The adsorption after 3 hours was less than 10%. The biodegradation of the test substance was determined to be less than 6% after 28 days. Thus, the test item is not considered to be inherently biodegradable under the test conditions chosen.

Additionally, the biodegradation of the test item was determined in a static test after 3 hours, 3, 5, 10 and 28 days. The degree of biodegradation was 0% after an exposure of 28 days. The EC50 value of the toxicity control was determined to be greater than 500 mg/L. Thus, the test item is not considered to be biodegrdable under the test conditions chosen.

In conclusion, the test is not considered to be biodegradable under the conditions chosen.