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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: No GLP defined.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1986

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
1,5-naphthylenediamine
EC Number:
218-817-8
EC Name:
1,5-naphthylenediamine
Cas Number:
2243-62-1
Molecular formula:
C10H10N2
IUPAC Name:
1,5-naphthylenediamine
Details on test material:
IUCLID4 Test substance: other TS: purity 98.9 %

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: one eye of each animal remained untreated and served as control
Amount / concentration applied:
100 µl (60 mg)
Duration of treatment / exposure:
24 h
Observation period (in vivo):
21 d
Number of animals or in vitro replicates:
3 animals

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: 1, 24, 48, 72 h, 7, 14, 21 days
Score:
0
Max. score:
4
Reversibility:
fully reversible
Irritation parameter:
iris score
Basis:
mean
Time point:
other: 1,24,48,72h, 14, 21 days
Score:
0
Max. score:
2
Reversibility:
fully reversible
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 1,24,48,72h, 14, 21 days
Score:
0
Max. score:
3
Reversibility:
fully reversible
Other effects:
no other effects reported

Any other information on results incl. tables

Eye irritation, rabbit (Exp.: 24h)

 Animal No.,Sex, b.w.  examined organs  symptoms                    DRAIZE -Grade in  Irritation values
       1h  24h  48h  72h  7d  14d  21d  
 E22, female (3,2 kg)  Cornea- cloudiness    0  0  0  0  0  -  - 0,0
    -surface    0  0  0  0  0  -  -  
   Iris    0  0  0  0  0  -  -  0,0
   Conjunctivae  R  1  0  0  0  0  -  -  0,0
     S  0  0  0  0  0  -  -  0,0
     T  0  0  0  0  0  -  -  
 E23, female (3,4 kg)  Cornea- cloudiness    0  0  0  0  0  -  -  0,0
    - surface    0  0  0  0  0  -  -  
   Iris    0  0  0  0  0  -  -  0,0
   Conjunctivae  R  0  0  0  0  0  -  -  0,0
     S  0  0  0  0  0  -  -  0,0
     T  0  0  0  0  0  -  -  
 E43, female (3,0 kg)  Cornea- cloudiness   0   0  0  0  0  - -   0,0
    - surface    0  0  0  0  0  -  -
   Iris    0  0  0  0  0  -  -  0,0
   Conjunctivae  R  0  0  0  0  0  -  -  0,0
     S  0  0  0  0  0  -  -  0,0
     T  0  0  1  0  0  -  -  

R= redeness

S= swelling

T= lacrimal flow

- = not examined

Applicant's summary and conclusion

Interpretation of results:
not irritating
Remarks:
Migrated information
Conclusions:
Classification: not irritating
Executive summary:

100 µl (ca. 60 mg) of test material was applied to one eye of 3 rabbits.

The exposure time was 24 h and the observation time 21 d.

No reddening, swelling or lacrimal flow was observed.

The test substance was not irritating to the eye.