Potassium titanium oxide (K1.88-2.13Ti8O16.94-17.07)

Long-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

05 July 2004 - 26 July 2004

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 211 (Daphnia magna Reproduction Test)

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

100% purity

Analytical monitoring:

yes

Details on sampling:

Samples were taken from freshly prepared solutions on nominal days 2, 7, 14, and 18. And from 48 or 72-hour old solutions on nominal days 4, 9, 16, and 21.

Vehicle:

yes

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- superstaurated solutions prepared weekly at nominally 100mg/l.
- magnetically stirred for a minimum of three days
- test solutions were filtered to remove the majority of the non-dissolved fraction

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna
- Justification for species other than prescribed by test guideline: internationally accepted invertebrate species
- Age at study initiation: <24 hours

- Method of breeding: 250 daphnids into 5 litres of medium
- Source: in house cultures
- Age of parental stock (mean and range, SD): < 4 weeks
- Feeding during test : daily
- Food type: algae

Test type:

semi-static

Water media type:

freshwater

Limit test:

yes

Total exposure duration:

21 d

Post exposure observation period:

not specified

Hardness:

290-360 (mg/L CaCO3)

Test temperature:

19.5 - 21.0°C

pH:

7.5 - 8.7

Dissolved oxygen:

7.5 - 10.3 mg/L

Salinity:

n/a

Conductivity:

n/a

Nominal and measured concentrations:

nominal 100 mg/L
measured concentrations during the study: 3.0 - 3.7 mg/L

Details on test conditions:

TEST SYSTEM
- Test vessel: 50 mL all glass
- Type (delete if not applicable): closed with a perspex plate
- Renewal rate of test solution (frequency/flow rate): three times a week
- No. of organisms: 20 neonate daphnids used per test group
- No. of vessels per concentration (replicates): individually exposed daphnids
- No. of vessels per control (replicates): 20

OTHER TEST CONDITIONS
- Photoperiod: 16 hours
- Light intensity: 700-796lux

Reference substance (positive control):

no

Key result

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Key result

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Details on results:

- Behavioural abnormalities: n/a
- Observations on body length: only 3% difference between control and test item groups
- Other biological observations: no aborted eggs in any groups were observed, no immobilised or dead offspring
- Mortality of control: 15% mortality

Results with reference substance (positive control):

n/a

Reported statistics and error estimates:

2-sample t-test

Validity criteria fulfilled:

yes

Conclusions:

No effects on survival or reproductive capacity were recorded at the maximum soluble concentration of terracess L in test medium

Reference 2

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Justification for type of information:

1. HYPOTHESIS FOR THE ANALOGUE APPROACH
Please see attached justification.
2. SOURCE AND TARGET CHEMICAL(S) (INCLUDING INFORMATION ON PURITY AND IMPURITIES)
Please see attached justification.

3. ANALOGUE APPROACH JUSTIFICATION
Please see attached justification.

4. DATA MATRIX
Please see attached justification.

Reason / purpose for cross-reference:

read-across source

Key result

Duration:

21 d

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Key result

Duration:

21 d

Dose descriptor:

LOEC

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Key result

Duration:

21 d

Dose descriptor:

NOEC

Effect conc.:

100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

reproduction

Description of key information

No effects on survival or reproductive capacity were recorded at the maximum soluble concentration of terracess L in test medium.

The same results are expected for Terracess TF due to the similarity of Terracess L.