Dinonylnaphthalenedisulphonic acid

Bioaccumulation: aquatic / sediment

Administrative data

Endpoint:

bioaccumulation in aquatic species, other

Type of information:

experimental study

Adequacy of study:

weight of evidence

Study period:

July 2 - November 9, 1979

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: limited reported test, non-GLP, no information on individual samples, limited in-life and exposure conditions. The test is indicative for a low bioaccumulation

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: "Method for Testing the Degree of Accumulation of Chemical Substances in Fish Body" in "Testing Methods for New Chemical Substances" (July 13, 1974), MHW. MITI

Deviations:

not specified

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

di C8-C10, branched, C9 rich, alkylnaphthalene disulphonic acid

IUPAC Name:

di C8-C10, branched, C9 rich, alkylnaphthalene disulphonic acid

Test material form:

solid: particulate/powder

Remarks:

migrated information: powder

Details on test material:

- Physical state: brown powder

Radiolabelling:

no

Sampling and analysis

Details on sampling:

- Sampling intervals/frequency for test organisms: every two weeks for 8 weeks
- Sampling intervals/frequency for test medium samples: every two weeks for 8 weeks
- Sample storage conditions before analysis: no data
- Details on sampling and analysis of test organisms and test media samples:
1) test water: after sampling hydrochloric acid and sodium chloride were added, thereafter samples were shaken twice with trioctylmethylammonium chloride in chloroform. The water layer was decanted and the organic fraction was filtrated (dehydration), dried (evaporation), resuspended in methanol and analysed by HPLC
2) fish: fish were weighed and measured before chopped. Thereafter they were suspended in methanol and further homogenized. This procedure was repeated twice. The suspension was filtered after each treatment. The filtrate was eluted via column chromatograpy. The eluate was evaporated (to 20-30 mL). Thereafter deionized water and sodium chloride were added and the sample was shaken with trioctylmethylammonium chloride in chloroform. The water layer was decanted and the organic fraction was filtrated (dehydration), dried (evaporation), resuspended in methanol and analysed by HPLC.

Test solutions

Vehicle:

no

Details on preparation of test solutions, spiked fish food or sediment:

PREPARATION AND APPLICATION OF TEST SOLUTION: stock solution was prepared in water, 2 g test substance in 2 L. This stock solution was further diluted to obtain the test concentrations

Test organisms

Test organisms (species):

other: carp

Details on test organisms:

TEST ORGANISM
- Common name: carp
- Strain: not indicated
- Source: not indicated
- Length at study initiation : 10.6 cm
- Weight at termination: 30.7 g
- Description of housing: 100 L tank
- Feeding during test: not indicated

ACCLIMATISATION: at 25 °C for 14 days ; kept in aqueous solution with 10 mg/L chlorotetracycline hydrochloride for 24 hours

Study design

Route of exposure:

aqueous

Test type:

flow-through

Water / sediment media type:

natural water: freshwater

Total exposure / uptake duration:

>= 2 - <= 8 wk

Test conditions

Test temperature:

25 ± 1 °C

Details on test conditions:

TEST SYSTEM
- Test vessel: 100L
- Type of flow-through: continuous flow
- Renewal rate of test solution: 575 L/day
- No. of organisms per vessel: No data

TEST MEDIUM / WATER PARAMETERS: no data

Nominal and measured concentrations:

nominal concentrations: 1 and 0.1 mg/L
measured concentration: at 2, 4, 6, and 8 weeks: 0.947, 0.964, 0.980 and 0.987 mg/L; 0.0962, 0.0961,,0.0953 and 0.0973 mg/L

Results and discussion

Bioaccumulation factor

Bioaccumulation factor 1

Type:

BCF

Value:

<= 0.19 dimensionless

Basis:

whole body w.w.

Calculation basis:

other: average over 8 week period

Remarks on result:

other: Conc.in environment / dose:1 mg/L

Bioaccumulation factor 2

Type:

BCF

Value:

<= 2 dimensionless

Basis:

whole body w.w.

Calculation basis:

other: average over 8 week period

Remarks on result:

other: Conc.in environment / dose:0.1 mg/L

Details on results:

Fish appeared normal

Any other information on results incl. tables

BCF values

Exposure duration	2 week	4 week	6 week	8 week
1 mg/L	≤0.19	≤0.19	≤0.19	≤0.19
0.1 mg/L	≤ 2.0	≤ 2.0	≤ 2.0	≤ 2.0

Applicant's summary and conclusion

Conclusions:

The bioconcentration factor of the test substance is ≤ 2.0

Executive summary:

Carp were exposed to the test substance at 1 and 0.1 mg/L in a flow-through test. Analyses of the test water and a sample of the fish were performed after 2, 4, 6, and 8 weeks. The Bioconcentration factor was assessed to be ≤ 0.19 at 1 mg/L and ≤ 2.0 at 0.1 mg/L.

The test substance does not bioaccumulate in fish.